Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential. (2019)
Journal Article
Smith, A., Hearn, J., Taylor, C., Wheelhouse, N., Kaczmarek, M., Moorhouse, E., & Singleton, I. (2019). Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential. International Journal of Food Microbiology, 299, 23-32. https://doi.org/10.1016/j.ijfoodmicro.2019.03.013

Listeria monocytogenes is sporadically detected on a range of ready to eat fresh produce lines, such as spinach and rocket, and is a threat to public health. However, little is known about the diversity of L. monocytogenes present on fresh produce an... Read More about Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential..

Morphology and molecular systematic of marine gregarines (Apicomplexa) from Southwestern Atlantic spionid polychaetes (2018)
Journal Article
Rueckert, S., Glasinovich, N., Diez, M., Cremonte, F., & Vázquez, N. (2018). Morphology and molecular systematic of marine gregarines (Apicomplexa) from Southwestern Atlantic spionid polychaetes. Journal of Invertebrate Pathology, 159, 49-60. https://doi.org/10.1016/j.jip.2018.10.010

Gregarines are a common group of parasites that infect the intestines of marine invertebrates, and particularly polychaetes. Here, we describe for the first time four gregarine species that inhabit the intestines of three spionid species: Dipolydora... Read More about Morphology and molecular systematic of marine gregarines (Apicomplexa) from Southwestern Atlantic spionid polychaetes.

Revisions to the Classification, Nomenclature, and Diversity of Eukaryotes (2018)
Journal Article
Adl, S. M., Bass, D., Lane, C. E., Lukeš, J., Schoch, C. L., Smirnov, A., Agatha, S., Berney, C., Brown, M. W., Burki, F., Cárdenas, P., Čepička, I., Chistyakova, L., del Campo, J., Dunthorn, M., Edvardsen, B., Eglit, Y., Guillou, L., Hampl, V., Heiss, A. A., …Zhang, Q. (2019). Revisions to the Classification, Nomenclature, and Diversity of Eukaryotes. Journal of Eukaryotic Microbiology, 66(1), 4-119. https://doi.org/10.1111/jeu.12691

This revision of the classification of eukaryotes follows that of Adl et al., 2012 [J. Euk. Microbiol. 59(5)] and retains an emphasis on protists. Changes since have improved the resolution of many nodes in phylogenetic analyses. For some clades even... Read More about Revisions to the Classification, Nomenclature, and Diversity of Eukaryotes.

Sources and survival of Listeria monocytogenes on fresh, leafy produce (2018)
Journal Article
on fresh, leafy produce. Journal of Applied Microbiology, 125(4), 930-942. https://doi.org/10.1111/jam.14025

Listeria monocytogenes in an intracellular human pathogen which enters the body through contaminated food stuffs and is known to contaminate fresh leafy produce such as spinach, lettuce and rocket. Routinely, fresh leafy produce is grown and processe... Read More about Sources and survival of Listeria monocytogenes on fresh, leafy produce.

Investigation of STM3071 as a potential regulator of cobalt transport in Salmonella enterica (2018)
Thesis
Piotrowska, A. A. Investigation of STM3071 as a potential regulator of cobalt transport in Salmonella enterica. (Thesis). Edinburgh Napier University. http://researchrepository.napier.ac.uk/Output/1253543

Using bioinformatics we have identified stm3071 as a possible regulator of anaerobically induced genes involved in metal homeostasis (Price-Carter et al., 2001) and the aim of this study is to determine the function of stm3071 and define the conditio... Read More about Investigation of STM3071 as a potential regulator of cobalt transport in Salmonella enterica.

Estimating excess length of stay due to healthcare-associated infections: A systematic review and meta-analysis of statistical methodology (2018)
Journal Article
Manoukian, S., Stewart, S., Dancer, S., Graves, N., Mason, H., McFarland, A., Robertson, C., & Reilly, J. (2018). Estimating excess length of stay due to healthcare-associated infections: A systematic review and meta-analysis of statistical methodology. Journal of Hospital Infection, https://doi.org/10.1016/j.jhin.2018.06.003

Background
Healthcare-associated infection (HAI) affects millions of patients worldwide. HAI is associated with increased healthcare costs, owing primarily to increased hospital length of stay (LOS) but calculating these costs is complicated due to... Read More about Estimating excess length of stay due to healthcare-associated infections: A systematic review and meta-analysis of statistical methodology.

Quantifying the relative effect of environmental contamination on surgical ward MRSA incidence: An exploratory analysis (2018)
Journal Article
Lee, X. J., Pettitt, A. N., & Dancer, S. J. (2018). Quantifying the relative effect of environmental contamination on surgical ward MRSA incidence: An exploratory analysis. Infection, Disease & Health, https://doi.org/10.1016/j.idh.2018.02.005

Background: To investigate and quantify the contribution of environmental contamination towards methicillin-resistant Staphylococcus aureus (MRSA) incidence observed in a hospital ward using stochastic modelling. Methods: A non-homogeneous Poisson pr... Read More about Quantifying the relative effect of environmental contamination on surgical ward MRSA incidence: An exploratory analysis.

Spatial zoning of microbial functions and plant-soil nitrogen dynamics across a riparian area in an extensively grazed livestock system (2018)
Journal Article
de Sosa, L. L., Glanville, H. C., Marshall, M. R., Williams, A. P., Abadie, M., Clark, I. M., Blaud, A., & Jones, D. L. (2018). Spatial zoning of microbial functions and plant-soil nitrogen dynamics across a riparian area in an extensively grazed livestock system. Soil Biology and Biochemistry, 120, 153-164. https://doi.org/10.1016/j.soilbio.2018.02.004

Anthropogenic activities have significantly altered global biogeochemical nitrogen (N) cycling leading to major environmental problems such as freshwater eutrophication, biodiversity loss and enhanced greenhouse gas emissions. The soils in the ripari... Read More about Spatial zoning of microbial functions and plant-soil nitrogen dynamics across a riparian area in an extensively grazed livestock system.

How Does a Photocatalytic Antimicrobial Coating Affect Environmental Bioburden in Hospitals? (2018)
Journal Article
Reid, M., Whatley, V., Spooner, E., Nevill, A. M., Cooper, M., Ramsden, J. J., & Dancer, S. J. (2018). How Does a Photocatalytic Antimicrobial Coating Affect Environmental Bioburden in Hospitals?. Infection Control and Hospital Epidemiology, 39(4), 398-404. https://doi.org/10.1017/ice.2017.297

BACKGROUND The healthcare environment is recognized as a source for healthcare-acquired infection. Because cleaning practices are often erratic and always intermittent, we hypothesize that continuously antimicrobial surfaces offer superior control of... Read More about How Does a Photocatalytic Antimicrobial Coating Affect Environmental Bioburden in Hospitals?.

Report of the 2017 Protistology-UK Spring Meeting (2017)
Presentation / Conference Contribution
Rueckert, S., & Tsaousis, A. D. Report of the 2017 Protistology-UK Spring Meeting

The Protistology-UK 2017 spring meeting was organised as part of the 2017 Annual Meeting of the Microbiology Society in Edinburgh between the 3rd to the 6th of April 2017. The meeting was held at the Edinburgh International Conference Centre (EICC)... Read More about Report of the 2017 Protistology-UK Spring Meeting.

Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain (2017)
Journal Article
Velasova, M., Damaso, A., Prakashbabu, B. C., Gibbons, J., Wheelhouse, N., Longbottom, D., Van Winden, S., Green, M., & Guitian, J. (2017). Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain. Journal of Dairy Science, 100(11), 9215-9233. https://doi.org/10.3168/jds.2016-11863

To implement appropriate and effective disease control programs at national level, up-to-date and unbiased information on disease frequency is needed. The aim of this study was to estimate the prevalence of selected endemic infectious diseases in th... Read More about Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain.

Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS (2017)
Journal Article
Livingstone, M., Wheelhouse, N., Ensor, H., Rocchi, M., Maley, S., Aitchison, K., Wattegedera, S., Wilson, K., Sait, M., Siarkou, V., Vretou, E., Entrican, G., Dagleish, M., & Longbottom, D. (2017). Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS. PLOS ONE, 12(5), Article e0177653. https://doi.org/10.1371/journal.pone.0177653

This study investigated the pathogenesis of two variant strains (LLG and POS) of Chlamydia abortus, in comparison to a typical wild-type strain (S26/3) which is known to be responsible for late term abortion in small ruminants. Challenge with the thr... Read More about Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS.

Evidence for rapid, tide-related shifts in the microbiome of the coral Coelastrea aspera (2017)
Journal Article
Sweet, M. J., Brown, B. E., Dunne, R. P., Singleton, I., & Bulling, M. (2017). Evidence for rapid, tide-related shifts in the microbiome of the coral Coelastrea aspera. Coral Reefs, 36(3), 815-828. https://doi.org/10.1007/s00338-017-1572-y

Shifts in the microbiome of the intertidal coral Coelastrea aspera (formally known as Goniastrea aspera) from Phuket, Thailand were noted over the course of a four day period of spring tides. During this time corals were naturally exposed to high tem... Read More about Evidence for rapid, tide-related shifts in the microbiome of the coral Coelastrea aspera.

Isolation of Indigenous Hydrocarbon Transforming Bacteria from Oil Contaminated Soils in Libya: Selection for Use as Potential Inocula for Soil Bioremediation (2017)
Journal Article
Hakima, A., & Singleton, I. (2017). Isolation of Indigenous Hydrocarbon Transforming Bacteria from Oil Contaminated Soils in Libya: Selection for Use as Potential Inocula for Soil Bioremediation. International journal of environmental bioremediation & biodegradation, 5(1), 8-17. https://doi.org/10.12691/ijebb-5-1-2

The Libyan oil industry has left a significant legacy of contamination and methods are required to remediate oil-contaminated soils in the area. In this work hydrocarbon utilizing microorganisms were isolated and identified from contaminated soil sam... Read More about Isolation of Indigenous Hydrocarbon Transforming Bacteria from Oil Contaminated Soils in Libya: Selection for Use as Potential Inocula for Soil Bioremediation.

Megasonic sonication for cost-effective and automatable elution of Cryptosporidium from filters and membranes (2015)
Journal Article
Kerrouche, A., Desmulliez, M. P., & Bridle, H. (2015). Megasonic sonication for cost-effective and automatable elution of Cryptosporidium from filters and membranes. Journal of Microbiological Methods, 118, 123-127. https://doi.org/10.1016/j.mimet.2015.09.001

Sample processing is a highly challenging stage in the monitoring of waterborne pathogens. This step is time-consuming, requires highly trained technicians and often results in low recovery rates of pathogens. In the UK but also in other parts of the... Read More about Megasonic sonication for cost-effective and automatable elution of Cryptosporidium from filters and membranes.

Molecular systematics of marine gregarine apicomplexans from Pacific tunicates, with descriptions of five novel species of Lankesteria (2015)
Journal Article
Rueckert, S., Wakeman, K. C., Jenke-Kodama, H., & Leander, B. S. (2015). Molecular systematics of marine gregarine apicomplexans from Pacific tunicates, with descriptions of five novel species of Lankesteria. International Journal of Systematic and Evolutionary Microbiology, 65(8), 2598-2614. https://doi.org/10.1099/ijs.0.000300

The eugregarines are a group of apicomplexan parasites that mostly infect the intestines of invertebrates. The high level of morphological variation found within and among species of eugregarines makes it difficult to find consistent and reliable tra... Read More about Molecular systematics of marine gregarine apicomplexans from Pacific tunicates, with descriptions of five novel species of Lankesteria.

Analysing the effect of soil organic matter on bacterial communities using T-RFLP fingerprinting: different methods, different stories? (2015)
Journal Article
Blaud, A., Diouf, F., Herrmann, A. M., & Lerch, T. Z. (2015). Analysing the effect of soil organic matter on bacterial communities using T-RFLP fingerprinting: different methods, different stories?. Biology and Fertility of Soils, 51(8), 959-971. https://doi.org/10.1007/s00374-015-1041-0

Soil microbial ecology needs robust tools to elucidate ecological questions, such as the impact of fertilisation on soil microbial communities. However, the methods and data analysis used can directly affect the biological conclusions. In this study,... Read More about Analysing the effect of soil organic matter on bacterial communities using T-RFLP fingerprinting: different methods, different stories?.

The Enterococci (2014)
Book Chapter
Heimer, S. R., Morrison, D., & Gilmore, M. S. (2014). The Enterococci. In Y.-W. Tang, M. Sussman, D. Liu, & I. Poxton (Eds.), Molecular Medical Microbiology (717-730). (2nd revised). Academic Press

No abstract available.

Waddlia chondrophila Infects and Multiplies in Ovine Trophoblast Cells Stimulating an Inflammatory Immune Response (2014)
Journal Article
Wheelhouse, N., Coyle, C., Barlow, P. G., Mitchell, S., Greub, G., Baszler, T., Rae, M. T., & Longbottom, D. (2014). Waddlia chondrophila Infects and Multiplies in Ovine Trophoblast Cells Stimulating an Inflammatory Immune Response. PLOS ONE, 9(7), Article e102386. https://doi.org/10.1371/journal.pone.0102386

BACKGROUND

Waddlia chondrophila (W. chondrophila) is an emerging abortifacient organism which has been identified in the placentae of humans and cattle. The organism is a member of the order Chlamydiales, and shares many similarities at the genome... Read More about Waddlia chondrophila Infects and Multiplies in Ovine Trophoblast Cells Stimulating an Inflammatory Immune Response.

Genome sequencing and comparative analysis of three Chlamydia pecorum strains associated with different pathogenic outcomes (2014)
Journal Article
Sait, M., Livingstone, M., Clark, E. M., Wheelhouse, N., Spalding, L., Markey, B., Magnino, S., Lainson, F. A., Myers, G. S. A., & Longbottom, D. (2014). Genome sequencing and comparative analysis of three Chlamydia pecorum strains associated with different pathogenic outcomes. BMC Genomics, 15, 23. https://doi.org/10.1186/1471-2164-15-23

BACKGROUND: Chlamydia pecorum is the causative agent of a number of acute diseases, but most often causes persistent, subclinical infection in ruminants, swine and birds. In this study, the genome sequences of three C. pecorum strains isolated from t... Read More about Genome sequencing and comparative analysis of three Chlamydia pecorum strains associated with different pathogenic outcomes.