Dr Abdelfateh Kerrouche A.Kerrouche@napier.ac.uk
Associate Professor
Megasonic sonication for cost-effective and automatable elution of Cryptosporidium from filters and membranes
Kerrouche, Abdelfateh; Desmulliez, Marc P.Y.; Bridle, Helen
Authors
Marc P.Y. Desmulliez
Helen Bridle
Abstract
Sample processing is a highly challenging stage in the monitoring of waterborne pathogens. This step is time-consuming, requires highly trained technicians and often results in low recovery rates of pathogens. In the UK but also in other parts of the world, Cryptosporidium is the only pathogen directly tested for in routine operational monitoring. The traditional sampling process involves the filtration of 1000 L of water, semi-automated elution of the filters and membranes with recovery rates of about 30–40% typically. This paper explores the use of megasonic sonication in an attempt to increase recovery rates and reduce both the time required for processing and the number of labour-intensive steps. Results demonstrate that megasonic energy assisted elution is equally effective as the traditional manual process in terms of recovery rates. Major advantages are however offered in terms of reduction of the elution volume enabling the current centrifugation stage to be avoided. This saves time, equipment and staff costs and critically removes the step in the process that would be most challenging to automate, paving the way thereby for highly effective automated solutions to pathogens monitoring.
Citation
Kerrouche, A., Desmulliez, M. P., & Bridle, H. (2015). Megasonic sonication for cost-effective and automatable elution of Cryptosporidium from filters and membranes. Journal of Microbiological Methods, 118, 123-127. https://doi.org/10.1016/j.mimet.2015.09.001
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 3, 2015 |
Online Publication Date | Sep 10, 2015 |
Publication Date | 2015-11 |
Deposit Date | Feb 26, 2018 |
Publicly Available Date | Feb 26, 2018 |
Journal | Journal of Microbiological Methods |
Print ISSN | 0167-7012 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 118 |
Pages | 123-127 |
DOI | https://doi.org/10.1016/j.mimet.2015.09.001 |
Keywords | Cryptosporidium, Elution, Megasonic agitation, Sonication, Filtration, Waterborne pathogens, Monitoring |
Public URL | http://researchrepository.napier.ac.uk/Output/1052466 |
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Copyright Statement
© . This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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