Phosphomimetic Mutation of the N-Terminal Lid of MDM2 Enhances the Polyubiquitination of p53 through Stimulation of E2-Ubiquitin Thioester Hydrolysis (2014)
Journal Article
Fraser, J. A., Worrall, E. G., Lin, Y., Landre, V., Pettersson, S., Blackburn, E., Walkinshaw, M., Muller, P., Vojtesek, B., Ball, K., & Hupp, T. R. (2015). Phosphomimetic Mutation of the N-Terminal Lid of MDM2 Enhances the Polyubiquitination of p53 through Stimulation of E2-Ubiquitin Thioester Hydrolysis. Journal of Molecular Biology, 427(8), 1728-1747. https://doi.org/10.1016/j.jmb.2014.12.011

Mouse double minute 2 (MDM2) has a phosphorylation site within a lid motif at Ser17 whose phosphomimetic
mutation to Asp17 stimulates MDM2-mediated polyubiquitination of p53. MDM2 lid deletion, but not Asp17
mutation, induced a blue shift in the λm... Read More about Phosphomimetic Mutation of the N-Terminal Lid of MDM2 Enhances the Polyubiquitination of p53 through Stimulation of E2-Ubiquitin Thioester Hydrolysis.

The BioGRID interaction database: 2015 update (2014)
Journal Article
Chatr-aryamontri, A., Breitkreutz, B.-J., Oughtred, R., Boucher, L., Heinicke, S., Chen, D., Stark, C., Breitkreutz, A., Kolas, N., O'Donnell, L., Reguly, T., Nixon, J., Ramage, L., Winter, A., Sellam, A., Chang, C., Hirschman, J., Theesfeld, C., Rust, J., Livstone, M. S., …Tyers, M. (2015). The BioGRID interaction database: 2015 update. Nucleic Acids Research, 43(D1), D470-D478. https://doi.org/10.1093/nar/gku1204

The Biological General Repository for Interaction Datasets (BioGRID: http://thebiogrid.org) is an open access database that houses genetic and protein interactions curated from the primary biomedical literature for all major model organism species an... Read More about The BioGRID interaction database: 2015 update.

Formulation Pre-screening of Inhalation Powders Using Computational Atom–Atom Systematic Search Method (2014)
Journal Article
Ramachandran, V., Murnane, D., Hammond, R. B., Pickering, J., Roberts, K. J., Soufian, M., Forbes, B., Jaffari, S., Martin, G. P., Collins, E., & Pencheva, K. (2015). Formulation Pre-screening of Inhalation Powders Using Computational Atom–Atom Systematic Search Method. Molecular Pharmaceutics, 12(1), 18-33. https://doi.org/10.1021/mp500335w

The synthonic modeling approach provides a molecule-centered understanding of the surface properties of crystals. It has been applied extensively to understand crystallization processes. This study aimed to investigate the functional relevance of syn... Read More about Formulation Pre-screening of Inhalation Powders Using Computational Atom–Atom Systematic Search Method.

Identification and characterisation of an Old Yellow Enzyme (OYE) - NamA from Listeria monocytogenes (2014)
Thesis
Bamaga, M. A. Identification and characterisation of an Old Yellow Enzyme (OYE) - NamA from Listeria monocytogenes. (Thesis). Edinburgh Napier University. http://researchrepository.napier.ac.uk/id/eprint/9831

The food-borne pathogene Listeria monocytogenes has been considered a significant threat to human health worldwide. It mainly infects individuals suffering insuffecint immunity such as pregnant women. During pregnancy, L. monocytogenes is capable of... Read More about Identification and characterisation of an Old Yellow Enzyme (OYE) - NamA from Listeria monocytogenes.

Monocyte chemoattractant protein-induced protein 1 impairs adipogenesis in 3T3-L1 cells (2014)
Journal Article
Lipert, B., Wegrzyn, P., Sell, H., Eckel, J., Winiarski, M., Budzynski, A., Matlok, M., Kotlinowski, J., Ramage, L., Malecki, M., Wilk, W., Mitus, J., & Jura, J. (2014). Monocyte chemoattractant protein-induced protein 1 impairs adipogenesis in 3T3-L1 cells. BBA - Molecular Cell Research, 1843(4), 780-788. https://doi.org/10.1016/j.bbamcr.2014.01.001

Monocyte chemoattractant protein-induced protein 1 (MCPIP1) encoded by the ZC3H12a gene (also known as Regnase-1) is involved in the regulation of degradation of mRNA of inflammatory modulators and for processing of pre-miRNA. These functions depend... Read More about Monocyte chemoattractant protein-induced protein 1 impairs adipogenesis in 3T3-L1 cells.

Immortality, but not oncogenic transformation, of primary human cells leads to epigenetic reprogramming of DNA methylation and gene expression (2013)
Journal Article
Gordon, K., Clouaire, T., Bao, X. X., Kemp, S. E., Xenophontos, M., de Las Heras, J. I., & Stancheva, I. (2014). Immortality, but not oncogenic transformation, of primary human cells leads to epigenetic reprogramming of DNA methylation and gene expression. Nucleic Acids Research, 42(6), 3529-3541. https://doi.org/10.1093/nar/gkt1351

Tumourigenic transformation of normal cells into cancer typically involves several steps resulting in acquisition of unlimited growth potential, evasion of apoptosis and non-responsiveness to growth inhibitory signals. Both genetic and epigenetic cha... Read More about Immortality, but not oncogenic transformation, of primary human cells leads to epigenetic reprogramming of DNA methylation and gene expression.

Post-Transcriptional Regulation of Iron Homeostasis in Saccharomyces cerevisiae (2013)
Journal Article
Martínez-Pastor, M., De Llanos, R., Romero, A., & Puig, S. (2013). Post-Transcriptional Regulation of Iron Homeostasis in Saccharomyces cerevisiae. International Journal of Molecular Sciences, 14(8), 15785-15809. https://doi.org/10.3390/ijms140815785

Iron is an essential micronutrient for all eukaryotic organisms because it participates as a redox cofactor in a wide variety of biological processes. Recent studies in Saccharomyces cerevisiae have shown that in response to iron deficiency, an RNA-b... Read More about Post-Transcriptional Regulation of Iron Homeostasis in Saccharomyces cerevisiae.

Function and Regulation of Yeast Ribonucleotide Reductase: Cell Cycle, Genotoxic Stress, and Iron Bioavailability (2013)
Journal Article
Puig, S., Sanvisens, N., & Llanos, R. D. (2013). Function and Regulation of Yeast Ribonucleotide Reductase: Cell Cycle, Genotoxic Stress, and Iron Bioavailability. Biomedical Journal, 36(2), https://doi.org/10.4103/2319-4170.110398

All eukaryotic organisms require an adequate, balanced concentration of deoxyribonucleoside triphosphates (dNTPs) in order to assure accurate DNA replication and repair, and to maintain genomic integrity. The rate‑limiting step in dNTP synthesis is c... Read More about Function and Regulation of Yeast Ribonucleotide Reductase: Cell Cycle, Genotoxic Stress, and Iron Bioavailability.

Autophagic targeting of Src promotes cancer cell survival following reduced FAK signalling (2012)
Journal Article
Sandilands, E., Serrels, B., McEwan, D. G., Morton, J. P., Macagno, J. P., McLeod, K., Stevens, C., Brunton, V. G., Langdon, W. Y., Vidal, M., Sansom, O. J., Dikic, I., Wilkinson, S., & Frame, M. C. (2012). Autophagic targeting of Src promotes cancer cell survival following reduced FAK signalling. Nature Cell Biology, 14(1), 51-60. https://doi.org/10.1038/ncb2386

Here we describe a mechanism that cancer cells use to survive when flux through the Src/FAK pathway is severely perturbed. Depletion of FAK, detachment of FAK-proficient cells or expression of non-phosphorylatable FAK proteins causes sequestration of... Read More about Autophagic targeting of Src promotes cancer cell survival following reduced FAK signalling.

The BioGRID interaction database: 2013 update (2012)
Journal Article
Chatr-aryamontri, A., Breitkreutz, B.-J., Heinicke, S., Boucher, L., Winter, A., Stark, C., Nixon, J., Ramage, L., Kolas, N., O'Donnell, L., Reguly, T., Breitkreutz, A., Sellam, A., Chen, D., Chang, C., Rust, J., Livstone, M., Oughtred, R., Dolinski, K., & Tyers, M. (2013). The BioGRID interaction database: 2013 update. Nucleic Acids Research, 41(D1), D816-D823. https://doi.org/10.1093/nar/gks1158

The Biological General Repository for Interaction Datasets (BioGRID: http//thebiogrid.org) is an open access archive of genetic and protein interactions that are curated from the primary biomedical literature for all major model organism species. As... Read More about The BioGRID interaction database: 2013 update.

Ageing Increases Vulnerability to Aβ42 Toxicity in Drosophila (2012)
Journal Article
Rogers, I., Kerr, F., Martinez, P., Hardy, J., Lovestone, S., & Partridge, L. (2012). Ageing Increases Vulnerability to Aβ42 Toxicity in Drosophila. PLOS ONE, 7(7), Article e40569. https://doi.org/10.1371/journal.pone.0040569

Age is the major risk factor for many neurodegenerative diseases, including Alzheimer's Disease (AD), for reasons that are not clear. The association could indicate that the duration or degree of exposure to toxic proteins is important for pathology,... Read More about Ageing Increases Vulnerability to Aβ42 Toxicity in Drosophila.

The SUMO Pathway Promotes Basic Helix-Loop-Helix Proneural Factor Activity via a Direct Effect on the Zn Finger Protein Senseless (2012)
Journal Article
Chen, A., Powell, L. M., Huang, Y. C., Wang, P. Y., Kemp, S. E., & Jarman, A. P. (2012). The SUMO Pathway Promotes Basic Helix-Loop-Helix Proneural Factor Activity via a Direct Effect on the Zn Finger Protein Senseless. Molecular and Cellular Biology, 32(14), 2849-2860. https://doi.org/10.1128/mcb.06595-11

During development, proneural transcription factors of the basic helix-loop-helix (bHLH) family are required to commit cells to
a neural fate. In Drosophila neurogenesis, a key mechanism promoting sense organ precursor (SOP) fate is the synergy betw... Read More about The SUMO Pathway Promotes Basic Helix-Loop-Helix Proneural Factor Activity via a Direct Effect on the Zn Finger Protein Senseless.

Development of a method for the detection of GHB and other drugs using a handheld Raman Spectroscopy device (2012)
Thesis
O'Connor, L. Development of a method for the detection of GHB and other drugs using a handheld Raman Spectroscopy device. (Thesis). Edinburgh Napier University. http://researchrepository.napier.ac.uk/id/eprint/5260

The main piece of legislation which addresses illicit drugs in the UK is the Misuse of Drugs Act 1971. The Act was designed to prevent the use of drugs which are 'capable of having harmful effects sufficient to constitute a social problem'.
The Roa... Read More about Development of a method for the detection of GHB and other drugs using a handheld Raman Spectroscopy device.

Characterisation of class II and III fructose bisphosphatases in Clostridium acetobutylicum ATCC 824 (2012)
Thesis
Almohaish, A. A. Characterisation of class II and III fructose bisphosphatases in Clostridium acetobutylicum ATCC 824. (Thesis). Edinburgh Napier University. http://researchrepository.napier.ac.uk/id/eprint/5692

In low GC Gram-positive bacteria, the phenomenon of carbon catabolite repression is dependent on a metabolite-activated bifunctional protein kinase/phosphorylase. In Clostridium acetobutylicum ATCC 824, the hprK gene encodes the bifunctional protein... Read More about Characterisation of class II and III fructose bisphosphatases in Clostridium acetobutylicum ATCC 824.

Phosphorylation of the epidermal growth factor receptor (EGFR) is essential for interleukin-8 release from intestinal epithelial cells in response to challenge with Escherichia coli O157 :H7 flagellin. (2011)
Journal Article
Fraser-Pitt, D. J., Cameron, P., McNeilly, T. N., Boyd, A., Manson, E. D. T., & Smith, D. G. E. (2011). challenge with Escherichia coli O157 :H7 flagellin. Microbiology, 157, 2339-2347. https://doi.org/10.1099/mic.0.047670-0

Enterohaemorrhagic E. coli O157:H7 is a major food-borne and environmental pathogen responsible for both sporadic cases and outbreaks of food poisoning which can lead to serious sequelae, such as haemolytic uraemic syndrome. The structural subunit of... Read More about Phosphorylation of the epidermal growth factor receptor (EGFR) is essential for interleukin-8 release from intestinal epithelial cells in response to challenge with Escherichia coli O157 :H7 flagellin..

Characterisation of proteins extracted from the surface of Salmonella Typhimurium grown under SPI-2-inducing conditions by LC-ESI/MS/MS sequencing. (2011)
Journal Article
Sherry, A. E., Inglis, N. F., Stevenson, A., Fraser-Pitt, D. J., Everest, P., Smith, D. G. E., & Roberts, M. (2011). Characterisation of proteins extracted from the surface of Salmonella Typhimurium grown under SPI-2-inducing conditions by LC-ESI/MS/MS sequencing. Proteomics, 11, 361-370. https://doi.org/10.1002/pmic.200900802

Salmonella enterica has two pathogenicity islands encoding separate type three secretion systems (T3SS). Proteins secreted through these systems facilitate invasion and survival. After entry, Salmonella reside within a membrane bound vacuole, the Sal... Read More about Characterisation of proteins extracted from the surface of Salmonella Typhimurium grown under SPI-2-inducing conditions by LC-ESI/MS/MS sequencing..

Regulation and Function of the Original p53- Inducible p21 Gene (2010)
Book Chapter
Fraser, J. A. (2010). Regulation and Function of the Original p53- Inducible p21 Gene. In p53; Molecular Biology Intelligence Unit (100-116). Springer Science + Business Media. https://doi.org/10.1007/978-1-4419-8231-5_7

P21 is a well known regulator of cell cycle progression through its inhibitory actions on Cyclin dependent kinases, (Cdk)/cyclin complexes, and DNA replication via its binding to proliferating cell nuclear antigen (PCNA). p21 also has a role in many... Read More about Regulation and Function of the Original p53- Inducible p21 Gene.

Pattern browsing and query adjustment for the exploratory analysis and cooperative visualisation of microarray time-course data. (2010)
Presentation / Conference Contribution
Craig, P., Cannon, A., Kennedy, J., & Kukla, R. (2010, September). Pattern browsing and query adjustment for the exploratory analysis and cooperative visualisation of microarray time-course data. Presented at The 7th International Conference on Cooperative Design, Visualisation and Engineering

This paper presents work to support collaborative visualisation and data analysis in the microarray time-series explorer (MaTSE) software. We introduce a novel visualisation component called the ‘pattern browser’ which is used to support the annotati... Read More about Pattern browsing and query adjustment for the exploratory analysis and cooperative visualisation of microarray time-course data..

The binding ability of alpha-1-acid glycoprotein as a mechanism of resistance to methadone. (2010)
Thesis
Behan, J. L. The binding ability of alpha-1-acid glycoprotein as a mechanism of resistance to methadone. (Thesis). Edinburgh Napier University. http://researchrepository.napier.ac.uk/id/eprint/4446

Dependence on heroin and other opioids represents a considerable problem worldwide. There is a continual need to improve therapy and/ or find more efficacious alternatives if these issues are to be addressed. The most commonly implemented pharmacol... Read More about The binding ability of alpha-1-acid glycoprotein as a mechanism of resistance to methadone..

Tuberous sclerosis-2 (TSC2) regulates the stability of death-associated protein kinase-1 (DAPK) through a lysosome-dependent degradation pathway (2010)
Journal Article
Hupp, T., Lin, Y., Henderson, P., Pettersson, S., Satsangi, J., Hupp, T. R., & Stevens, C. (2010). Tuberous sclerosis-2 (TSC2) regulates the stability of death-associated protein kinase-1 (DAPK) through a lysosome-dependent degradation pathway. FEBS Journal, 278(2), 354-370. https://doi.org/10.1111/j.1742-4658.2010.07959.x

We previously identified a novel interaction between tuberous sclerosis-2 (TSC2) and death-associated protein kinase-1 (DAPK), the consequence being that DAPK catalyses the inactivating phosphorylation of TSC2 to stimulate mammalian target of rapamyc... Read More about Tuberous sclerosis-2 (TSC2) regulates the stability of death-associated protein kinase-1 (DAPK) through a lysosome-dependent degradation pathway.