Phosphorylation of the epidermal growth factor
receptor (EGFR) is essential for interleukin-8
release from intestinal epithelial cells in response to
challenge with Escherichia coli O157 :H7 flagellin.

Fraser-Pitt, Douglas J; Cameron, Pamela; McNeilly, Tom N; Boyd, Amanda; Manson, Erin D T; Smith, David G E

doi:10.1099/mic.0.047670-0

Phosphorylation of the epidermal growth factor
receptor (EGFR) is essential for interleukin-8
release from intestinal epithelial cells in response to
challenge with Escherichia coli O157 :H7 flagellin.

Fraser-Pitt, Douglas J; Cameron, Pamela; McNeilly, Tom N; Boyd, Amanda; Manson, Erin D T; Smith, David G E

Authors

Douglas J Fraser-Pitt

Pamela Cameron

Tom N McNeilly

Amanda Boyd

Erin D T Manson

Dr David Smith D.Smith@napier.ac.uk
Lecturer

Abstract

Enterohaemorrhagic E. coli O157:H7 is a major food-borne and environmental pathogen responsible for both sporadic cases and outbreaks of food poisoning which can lead to serious sequelae, such as haemolytic uraemic syndrome. The structural subunit of E. coli O157:H7 flagella is flagellin, which is both the antigenic determinant of the H7 serotype, an important factor in colonisation, and an immunomodulatory protein determined to be a major pro-inflammatory component through the instigation of host cell signalling pathways. Flagellin has highly conserved N and C-terminal regions that are recognised by the host cell pattern recognition receptor, toll-like receptor 5 (TLR5). Activation of this receptor triggers cell signalling cascades, which are known to activate host cell kinases and transcription factors that respond with the production of inflammatory mediators such as the chemokine interleukin-8 (IL-8), although the exact components of this pathway are not yet fully characterised. We demonstrate that E. coli O157:H7-derived flagellin induces rapid phosphorylation of the EGFR, as an early event in intestinal epithelial cell signalling, and that this is required for the release of pro-inflammatory cytokine IL-8.

Citation

Fraser-Pitt, D. J., Cameron, P., McNeilly, T. N., Boyd, A., Manson, E. D. T., & Smith, D. G. E. (2011). challenge with Escherichia coli O157 :H7 flagellin. Microbiology, 157, 2339-2347. https://doi.org/10.1099/mic.0.047670-0

Journal Article Type	Article
Acceptance Date	May 3, 2011
Publication Date	2011-05
Deposit Date	Jun 22, 2012
Print ISSN	1350-0872
Publisher	Microbiology Society
Peer Reviewed	Peer Reviewed
Volume	157
Pages	2339-2347
DOI	https://doi.org/10.1099/mic.0.047670-0
Keywords	EHEC; E. coli O157:H7; flagellin; EGFR; TLR5; IL-8;
Public URL	http://researchrepository.napier.ac.uk/id/eprint/5481
Publisher URL	http://dx.doi.org/10.1099/mic.0.047670-0