Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential (2019)
Journal Article
Sharma, A., Goring, A., Staines, K., Emery, R., Pitsillides, A. A., Oreffo, R. O., Mahajan, S., & Clarkin, C. E. (2020). Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential. Matrix Biology Plus, 5, https://doi.org/10.1016/j.mbplus.2019.100018

Mineralization of bone is achieved by the sequential maturation of the immature amorphous calcium phase to mature hydroxyapatite and is central to the process of bone development and repair. To study normal and dysregulated mineralization in vitro, s... Read More about Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential.

Development of a Caenorhabditis elegans model for the assessment of toxicity and its application in testing novel anthelmintics. (2017)
Thesis
Oluwadare, E. Development of a Caenorhabditis elegans model for the assessment of toxicity and its application in testing novel anthelmintics. (Thesis). Edinburgh Napier University. http://researchrepository.napier.ac.uk/Output/1036075

The nematode Caenorhabditis elegans is an alternative model used in biomedical research for the investigation of descriptive and mechanistic toxicity assessment of chemicals. There are considerable differences in published data, especially in terms o... Read More about Development of a Caenorhabditis elegans model for the assessment of toxicity and its application in testing novel anthelmintics..

Disastrous performance of NanoCote/Aqua Based antimicrobial paint in a hospital setting (2016)
Journal Article
Ramsden, J., Reid, M., Whatley, V., & Dancer, S. (2016). Disastrous performance of NanoCote/Aqua Based antimicrobial paint in a hospital setting. Journal of Biological Physics and Chemistry, 16(3), 131-136. https://doi.org/10.4024/16ra16a.jbpc.16.03

Attempts are being made to incorporate copper into clear varnish in order to coat environmental surfaces in hospitals with the long-term goal of reducing healthcare-acquired infections (HAI). The performance of a nanocopper-containing polyacrylic emu... Read More about Disastrous performance of NanoCote/Aqua Based antimicrobial paint in a hospital setting.

Immortality, but not oncogenic transformation, of primary human cells leads to epigenetic reprogramming of DNA methylation and gene expression (2013)
Journal Article
Gordon, K., Clouaire, T., Bao, X. X., Kemp, S. E., Xenophontos, M., de Las Heras, J. I., & Stancheva, I. (2014). Immortality, but not oncogenic transformation, of primary human cells leads to epigenetic reprogramming of DNA methylation and gene expression. Nucleic Acids Research, 42(6), 3529-3541. https://doi.org/10.1093/nar/gkt1351

Tumourigenic transformation of normal cells into cancer typically involves several steps resulting in acquisition of unlimited growth potential, evasion of apoptosis and non-responsiveness to growth inhibitory signals. Both genetic and epigenetic cha... Read More about Immortality, but not oncogenic transformation, of primary human cells leads to epigenetic reprogramming of DNA methylation and gene expression.

Development of a method for the detection of GHB and other drugs using a handheld Raman Spectroscopy device (2012)
Thesis
O'Connor, L. Development of a method for the detection of GHB and other drugs using a handheld Raman Spectroscopy device. (Thesis). Edinburgh Napier University. http://researchrepository.napier.ac.uk/id/eprint/5260

The main piece of legislation which addresses illicit drugs in the UK is the Misuse of Drugs Act 1971. The Act was designed to prevent the use of drugs which are 'capable of having harmful effects sufficient to constitute a social problem'.
The Roa... Read More about Development of a method for the detection of GHB and other drugs using a handheld Raman Spectroscopy device.

Characterisation of class II and III fructose bisphosphatases in Clostridium acetobutylicum ATCC 824 (2012)
Thesis
Almohaish, A. A. Characterisation of class II and III fructose bisphosphatases in Clostridium acetobutylicum ATCC 824. (Thesis). Edinburgh Napier University. http://researchrepository.napier.ac.uk/id/eprint/5692

In low GC Gram-positive bacteria, the phenomenon of carbon catabolite repression is dependent on a metabolite-activated bifunctional protein kinase/phosphorylase. In Clostridium acetobutylicum ATCC 824, the hprK gene encodes the bifunctional protein... Read More about Characterisation of class II and III fructose bisphosphatases in Clostridium acetobutylicum ATCC 824.

The binding ability of alpha-1-acid glycoprotein as a mechanism of resistance to methadone. (2010)
Thesis
Behan, J. L. The binding ability of alpha-1-acid glycoprotein as a mechanism of resistance to methadone. (Thesis). Edinburgh Napier University. http://researchrepository.napier.ac.uk/id/eprint/4446

Dependence on heroin and other opioids represents a considerable problem worldwide. There is a continual need to improve therapy and/ or find more efficacious alternatives if these issues are to be addressed. The most commonly implemented pharmacol... Read More about The binding ability of alpha-1-acid glycoprotein as a mechanism of resistance to methadone..

Characterisation of glutamine fructose-6-phosphate amidotransferase (EC 2.6.1.16) and N-acetylglucosamine metabolism in Bifidobacterium (2007)
Journal Article
Aïssi, E., Foley, S., Stolarczyk, E., Mouni, F., Brassart, C., Vidal, O., Aissi, E., Bouquelet, S., & Krzewinski, F. (2008). Characterisation of glutamine fructose-6-phosphate amidotransferase (EC 2.6.1.16) and N-acetylglucosamine metabolism in Bifidobacterium. Archives of Microbiology, 189(2), 157-167. https://doi.org/10.1007/s00203-007-0307-9

Bifidobacterium bifidum, in contrast to other bifido-bacterial species, is auxotrophic for N-acetylglucosamine. Growth experiments revealed assimilation of radiolabelled N-acetylglucosamine in bacterial cell walls and in acetate, an end-product of ce... Read More about Characterisation of glutamine fructose-6-phosphate amidotransferase (EC 2.6.1.16) and N-acetylglucosamine metabolism in Bifidobacterium.

Reduced alveolar macrophage migration induced by acute ambient particle (PM10) exposure (2007)
Journal Article
Barlow, P. G., Brown, D. M., Donaldson, K., MacCallum, J., & Stone, V. (2008). Reduced alveolar macrophage migration induced by acute ambient particle (PM10) exposure. Cell Biology and Toxicology, 24(3), 243-252. https://doi.org/10.1007/s10565-007-9033-y

Increased levels of particulate air pollution (PM10) have been implicated as a causal agent in pulmonary disease exacerbation and increased deaths from respiratory and cardiovascular disorders. The exact mechanism by which PM10 drives toxicity in the... Read More about Reduced alveolar macrophage migration induced by acute ambient particle (PM10) exposure.

Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit (2007)
Journal Article
Tzortzakis, N., Singleton, I., & Barnes, J. (2008). Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit. Postharvest Biology and Technology, 47(1), 1-9. https://doi.org/10.1016/j.postharvbio.2007.06.004

Tomato fruit (Lycopersicon esculentum L.) were exposed to ozone concentrations between 0.005 (control) and 5.0 μmol mol−1 up to 13 days at 13 °C, prior to, or following, inoculation by Alternaria alternata or Colletotrichum coccodes (causes of black... Read More about Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit.

Nanoparticle interactions with zinc and iron: Implications for toxicology and inflammation (2007)
Journal Article
Wilson, M. R., Foucaud, L., Barlow, P. G., Hutchison, G. R., Sales, J., Simpson, R. J., & Stone, V. (2007). Nanoparticle interactions with zinc and iron: Implications for toxicology and inflammation. Toxicology and Applied Pharmacology, 225(1), 80-89. https://doi.org/10.1016/j.taap.2007.07.012

Particulate air pollution (PM10) consists of a mixture of components, including nanoparticles and metals. Studies from our laboratory have demonstrated that transition metals can potentiate the ability of nanoparticles to induce lung inflammation and... Read More about Nanoparticle interactions with zinc and iron: Implications for toxicology and inflammation.

Anaerobic biodegradation of linear alkylbenzene: isomeric ratio as a monitoring tool (2007)
Journal Article
Johnson, S. J., Barry, D., & Christofi, N. (2007). Anaerobic biodegradation of linear alkylbenzene: isomeric ratio as a monitoring tool. Land Contamination and Reclamation, 15(2), 235-241. https://doi.org/10.2462/09670513.858

Linear alkylbenzenes (LABs) are common environmental contaminants associated with a range of industrial and domestic activities. Although natural environments receiving LABs may exhibit a range of redox conditions, until now only aerobic biodegradati... Read More about Anaerobic biodegradation of linear alkylbenzene: isomeric ratio as a monitoring tool.

Thyroid hormone signaling in human ovarian surface epithelial cells. (2007)
Journal Article
Rae, M. T., Gubbay, O., Kostogiannou, A., Price, D., Critchley, H. O. D., & Hillier, S. G. (2007). Thyroid hormone signaling in human ovarian surface epithelial cells. Journal of Clinical Endocrinology and Metabolism, 92(1), 322-327. https://doi.org/10.1210/jc.2006-1522

Ovarian surface epithelial (OSE) cells express multiple nuclear hormone receptor genes, including those encoding thyroid hormone and estrogen receptors (TR and ER, respectively). Ovarian cancer is hormone-dependent, and epidemiological evidence links... Read More about Thyroid hormone signaling in human ovarian surface epithelial cells..

Characterisation of a glucose phosphotransferase system in Clostridium acetobutylicum ATCC 824 (2006)
Journal Article
Tangney, M., & Mitchell, W. J. (2007). Characterisation of a glucose phosphotransferase system in Clostridium acetobutylicum ATCC 824. Applied microbiology and biotechnology, 74(2), 398-405. https://doi.org/10.1007/s00253-006-0679-9

The transport of glucose by the solventogenic anaerobe Clostridium acetobutylicum was investigated. Glucose phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity was detected in extracts prepared from cultures grown on glucose... Read More about Characterisation of a glucose phosphotransferase system in Clostridium acetobutylicum ATCC 824.

The efficacy of certain anti-tuberculosis drugs is affected by binding to alpha-1-acid glycoprotein. (2006)
Journal Article
Johnson, D. A., & Smith, K. (2006). The efficacy of certain anti-tuberculosis drugs is affected by binding to alpha-1-acid glycoprotein. Biomedical Chromatography, 20, 551-560. https://doi.org/10.1002/bmc.641

One of the most ubiquitous plasma proteins, -1-acid glycoprotein (AGP), has a high affinity, low capacity binding for basic drugs positively charged at physiological pH. Moreover, as an acute phase protein its level is increased in various disease st... Read More about The efficacy of certain anti-tuberculosis drugs is affected by binding to alpha-1-acid glycoprotein..

Millisia brevis gen. nov., sp. nov., an actinomycete isolated from activated sludge foam. (2006)
Journal Article
Soddell, J. A., Stainsby, F. M., Eales, K. L., Kroppenstedt, R. M., Seviour, R. J., & Goodfellow, M. (2006). Millisia brevis gen. nov., sp. nov., an actinomycete isolated from activated sludge foam. International Journal of Systematic and Evolutionary Microbiology, 56, 739-744. https://doi.org/10.1099/ijs.0.63855-0

The taxonomic position of two mycolic-acid-producing actinomycetes, isolates J81T and J82, which were recovered from activated sludge foam, was clarified. Comparative 16S rRNA gene sequence studies indicated that the organisms formed a distinct linea... Read More about Millisia brevis gen. nov., sp. nov., an actinomycete isolated from activated sludge foam..

Hydrogenases in Desulfovibrio vulgaris Hildenborough: structural and physiologic characterisation of the membrane-bound [NiFeSe] hydrogenase (2005)
Journal Article
Valente, F. M. A., Oliveira, A. S. F., Gnadt, N., Pacheco, I., Coelho, A. V., Xavier, A. V., Teixeira, M., Soares, C. M., & Pereira, I. A. C. (2005). Hydrogenases in Desulfovibrio vulgaris Hildenborough: structural and physiologic characterisation of the membrane-bound [NiFeSe] hydrogenase. Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 10(6), 667-682. https://doi.org/10.1007/s00775-005-0022-4

The genome of Desulfovibrio vulgaris Hildenborough (DvH) encodes for six hydrogenases (Hases), making it an interesting organism to study the role of these proteins in sulphate respiration. In this work we address the role of the [NiFeSe] Hase, found... Read More about Hydrogenases in Desulfovibrio vulgaris Hildenborough: structural and physiologic characterisation of the membrane-bound [NiFeSe] hydrogenase.

The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil. (2005)
Journal Article
O’Mahony, M. M., Dobson, A. D., Barnes, J. D., & Singleton, I. (2006). The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil. Chemosphere, 63(2), 307-314. https://doi.org/10.1016/j.chemosphere.2005.07.018

The potential of using ozone for the removal of phenanthrene from several different soils, both alone and in combination with biodegradation using a microbial inoculant (Pseudomonas alcaligenes PA-10), was examined. The greater the water content of t... Read More about The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil..

Free-CoA mediated regulation of intermediary and central metabolism: An hypothesis, which accounts for the excretion of α-ketoglutarate during aerobic growth of E. coli on acetate (2005)
Journal Article
El-Mansi, M. (2005). Free-CoA mediated regulation of intermediary and central metabolism: An hypothesis, which accounts for the excretion of α-ketoglutarate during aerobic growth of E. coli on acetate. Research in Microbiology, 156(8), 874-879. https://doi.org/10.1016/j.resmic.2005.04.008

During growth of Escherichia coli on acetate, phosphotransacetylase and -ketoglutarate dehydrogenase are in direct competition for their common co-factor, HS-CoA. Such competition is resolved in favour of phosphotransacetylase, thus rendering -ketogl... Read More about Free-CoA mediated regulation of intermediary and central metabolism: An hypothesis, which accounts for the excretion of α-ketoglutarate during aerobic growth of E. coli on acetate.

Oxoazabenzo[de]anthracenes conjugated to Amino Acids: Synthesis and evaluation as DNA-binding antitumor agents. (2005)
Journal Article
Dias, N., Goossens, J.-F., Baldeyrou, B., Lansiaux, A., Colson, P., Di Salvo, A., Bernal, J., Turnbull, A., Mincher, D., & Bailly, C. (2005). Oxoazabenzo[de]anthracenes conjugated to Amino Acids: Synthesis and evaluation as DNA-binding antitumor agents. Bioconjugate Chemistry, 16(4), 949-958. https://doi.org/10.1021/bc050065x

We report the synthesis of an original series of oxoazabenzo[de]anthracenes conjugated to an amino acid: Ala, Phe, Pro, Lys, or Gly (4a-e, respectively). The compounds, derived from 1,8-dihydroxyanthracene-9,10-dione, were studied for DNA binding and... Read More about Oxoazabenzo[de]anthracenes conjugated to Amino Acids: Synthesis and evaluation as DNA-binding antitumor agents..