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Millisia brevis gen. nov., sp. nov., an actinomycete isolated from activated sludge foam.

Soddell, Jacques A; Stainsby, Fiona M; Eales, Kathryn L; Kroppenstedt, Reiner M; Seviour, Robert J; Goodfellow, Michael


Jacques A Soddell

Kathryn L Eales

Reiner M Kroppenstedt

Robert J Seviour

Michael Goodfellow


The taxonomic position of two mycolic-acid-producing actinomycetes, isolates J81T and J82, which were recovered from activated sludge foam, was clarified. Comparative 16S rRNA gene sequence studies indicated that the organisms formed a distinct lineage within the Corynebacterineae 16S rRNA gene tree. The taxonomic integrity of this group was underpinned by a wealth of phenotypic data, notably characteristic rudimentary right-angled branching. In addition, isolate J81T contained the following: meso-diaminopimelic acid, arabinose and galactose; N-glycolated muramic acid residues; a dihydrogenated menaquinone with eight isoprene units as the predominant isoprenologue; a fatty acid profile rich in oleic and palmitoleic acids and with relatively small proportions of myristic, stearic and tuberculostearic acids; mycolic acids with 44–52 carbons; and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides as major polar lipids. Strain J81T was found to have a chemotaxonomic profile that serves to distinguish it from representatives of all of the other taxa classified as belonging to the suborder Corynebacterineae. In the light of these data, it is proposed that the two isolates be classified in a novel monospecific genus. The name proposed for this taxon is Millisia brevis gen. nov., sp. nov.; strain J81T (=DSM 44463T=NRRL B-24424T) is the type strain of Millisia brevis.


Soddell, J. A., Stainsby, F. M., Eales, K. L., Kroppenstedt, R. M., Seviour, R. J., & Goodfellow, M. (2006). Millisia brevis gen. nov., sp. nov., an actinomycete isolated from activated sludge foam. International Journal of Systematic and Evolutionary Microbiology, 56, 739-744.

Journal Article Type Article
Publication Date Apr 1, 2006
Deposit Date Jul 17, 2008
Print ISSN 1466-5026
Electronic ISSN 1466-5034
Publisher Microbiology Society
Peer Reviewed Peer Reviewed
Volume 56
Pages 739-744
Keywords Foam; Activated sludge; Actinomycetes; Taxonomy; Chemical analysis; Corynebacterineae; Millisia brevis gen. nov., sp. nov. strain J81T (=DSM 44463T=NRRL B-24424T);
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