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Protein kinase C- and Ca2+ ionophore-stimulated production of reactive oxygen species in mechanically dispersed isolated bovine luteal cells (1997)
Journal Article
Sakka, E., Rae, M., Bramley, T., & Aitken, J. (1997). Protein kinase C- and Ca2+ ionophore-stimulated production of reactive oxygen species in mechanically dispersed isolated bovine luteal cells. Biology of Reproduction, 57(2), 428-435

We measured the production of reactive oxygen species (ROS) using luminol-horseradish peroxidase-induced chemiluminescence in mechanically dispersed cell suspensions from bovine corpus luteum (CL). Since other cell types besides luteal cells were pre... Read More about Protein kinase C- and Ca2+ ionophore-stimulated production of reactive oxygen species in mechanically dispersed isolated bovine luteal cells.

In Situ Hybridization Localization of the GABAA Receptor β2S- and β2L-Subunit Transcripts Reveals Cell-Specific Splicing of Alternate Cassette Exons (1997)
Journal Article
Harvey, R., & Darlison, M. (1997). In Situ Hybridization Localization of the GABAA Receptor β2S- and β2L-Subunit Transcripts Reveals Cell-Specific Splicing of Alternate Cassette Exons. Neuroscience, 77(2), 361-369. https://doi.org/10.1016/S0306-4522%2896%2900467-8

We have recently described two variants of the chicken {GABAA} receptor β2 subunit which arise by alternative splicing of the corresponding primary gene transcript. The long form of the β2 subunit (β2L) differs from the short form (β2S) by the insert... Read More about In Situ Hybridization Localization of the GABAA Receptor β2S- and β2L-Subunit Transcripts Reveals Cell-Specific Splicing of Alternate Cassette Exons.

GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness (1996)
Book Chapter
Darlison, M. G., & Harvey, R. J. (1996). GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness. In Molecular Biology of Membrane Transport Disorders (169-180). Springer. https://doi.org/10.1007/978-1-4613-1143-0_9

Rapid chemical communication between cells in the vertebrate central nervous system is mediated by ligand-gated ion-channel receptors (also called ionotropic receptors), which are multisubunit complexes, that each contain an ion-selective channel. In... Read More about GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness.

Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells (1996)
Journal Article
Bahn, S., Harvey, R. J., Darlison, M. G., & Wisden, W. (1996). Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells. Journal of Neurochemistry, 66(5), 1810-1818. https://doi.org/10.1046/j.1471-4159.1996.66051810.x

The γ-aminobutyric acid type A receptor cDNAs encoding the α6 subunit homologues from chicken and goldfish have been cloned and sequenced. These proteins exhibit 83 and 75% identity, respectively, to the rat α6 polypeptide. In situ hybridization has... Read More about Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells.

Exclusion of linkage between idiopathic generalized epilepsies and the GABAA receptor α1 and γ2 subunit gene cluster on chromosome 5 (1996)
Journal Article
Sander, T., Hildmann, T., Janz, D., Wienker, T. F., Bianchi, A., Bauer, G., Sailer, U., Scaramelli, A., Neitzel, H., Schmitz, B., Bailey, M. E., Beck-Mannagetta, G., Johnson, K. J., & Darlison, M. G. (1996). Exclusion of linkage between idiopathic generalized epilepsies and the GABAA receptor α1 and γ2 subunit gene cluster on chromosome 5. Epilepsy Research, 23(3), 235-244. https://doi.org/10.1016/0920-1211%2895%2900098-4

Hereditary factors play a major role in the etiology of idiopathic generalized epilepsies (IGEs). The pivotal function of ionotropic γ-aminobutyric acid type A receptors (GABRs) in inhibitory neurotransmission in the mammalian central nervous system... Read More about Exclusion of linkage between idiopathic generalized epilepsies and the GABAA receptor α1 and γ2 subunit gene cluster on chromosome 5.

Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed inXenopus oocytes from a cloned cDNA (1995)
Journal Article
Bhandal, N. S., Ramsey, R. L., Harvey, R. J., Darlison, M. G., & Usherwood, P. N. R. (1995). Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed inXenopus oocytes from a cloned cDNA. Invertebrate Neuroscience, 1(3), 267-272. https://doi.org/10.1007/bf02211028

We have previously described the isolation of a complementary DNA (cDNA) from the freshwater molluscLymnaea stagnalis encoding a polypeptide that exhibits ∼50{%} identity to the {ß}-subunits of vertebrate $γ$-aminobutyric acid (GABA) type A (GABAA) r... Read More about Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed inXenopus oocytes from a cloned cDNA.

The embryonal carcinoma cell line PCC7-S-AzaR: 21.21: 21.2 (clone 1009) expresses a functional GABA: 21.2A: 21.2 receptor comprising the α5, β3 and γ3 subunits: 21.2 (1995)
Journal Article
Harvey, R. J., Lobron, C., Reinhardt-Maelicke, S., Maelicke, A., & Darlison, M. G. (1995). The embryonal carcinoma cell line PCC7-S-AzaR: 21.21: 21.2 (clone 1009) expresses a functional GABA: 21.2A: 21.2 receptor comprising the α5, β3 and γ3 subunits: 21.2. Behavioural Pharmacology, 6(SUPPLEMENT 1), 119. https://doi.org/10.1097/00008877-199505001-00138

Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes (1995)
Journal Article
Albrecht, B. E., & Darlison, M. G. (1995). Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes. Neuroscience Letters, 189(3), 155-158. https://doi.org/10.1016/0304-3940%2895%2911479-g

We have amplified partial complementary {DNAs} for the chicken γ-aminobutyric acid type C (GABAC) receptor ρ1 and ρ2 subunits using the polymerase chain reaction. These nucleotide sequences have been utilized to design specific oligonucleotide probes... Read More about Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes.

The physiological demands of Gaelic football. (1995)
Journal Article
Florida-James, G., & Reilly, T. (1995). The physiological demands of Gaelic football. British Journal of Sports Medicine, 29(1), 41-45. doi:10.1136/bjsm.29.1.41

Match-lay demands of Gaelic football and fitness profiles were assessed at club competitive level. English Gaelic football club championship players (n = 11) were assessed for anthropometry, leg strength and time to exhaustion on a treadmill run. A s... Read More about The physiological demands of Gaelic football..

Isolation and sequence analysis of the chicken GABAa receptor α1-subunit gene promoter (1995)
Journal Article
Bateson, A. N., Ultsch, A., & Darlison, M. G. (1995). Isolation and sequence analysis of the chicken GABAa receptor α1-subunit gene promoter. Gene, 153(2), 243-247. https://doi.org/10.1016/0378-1119%2894%2900776-o

A genomic clone containing the 5'-flanking sequence of the chicken {GABAA} receptor α1-subunit-encoding gene (GabRα1) was isolated and characterized. An intron was found to interrupt the 5'-untranslated region. The transcription start point (tsp) was... Read More about Isolation and sequence analysis of the chicken GABAa receptor α1-subunit gene promoter.

Analysis of GABAA receptor subunit genes in multiplex pedigrees with manic depression (1994)
Journal Article
Coon, H., Hicks, A. A., Bailey, M. E., Hoff, M., Holik, J., Harvey, R. J., Johnson, K. J., Darlison, M. G., Reimherr, F., Wender, P., & Byerley, W. (1994). Analysis of GABAA receptor subunit genes in multiplex pedigrees with manic depression. Psychiatric Genetics, 4(3), 185-191. https://doi.org/10.1097/00041444-199400430-00009

The gamma-aminobutyric acid (GABA) neurotransmitter system has been implicated in the pathogenesis of manic depression. Tests of this hypothesis can now be carried out due to the recent characterization of simple sequence repeat polymorphisms for the... Read More about Analysis of GABAA receptor subunit genes in multiplex pedigrees with manic depression.

Developmental up-regulation and agonist-dependent down-regulation of GABAA receptor subunit mRNAs in chick cortical neurons (1994)
Journal Article
Barnes Jr., E. M., Baumgartner, B. J., Harvey, R. J., & Darlison, M. G. (1994). Developmental up-regulation and agonist-dependent down-regulation of GABAA receptor subunit mRNAs in chick cortical neurons. Molecular Brain Research, 26(1-2), 9-17. https://doi.org/10.1016/0169-328x%2894%2990068-x

Research report
We have used quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to analyze the expression of {GABAA} receptor subunit genes in cultured neurons from the chick embryo cerebral cortex. During maturation of the neuro... Read More about Developmental up-regulation and agonist-dependent down-regulation of GABAA receptor subunit mRNAs in chick cortical neurons.

Sequence of a Drosophila Ligand-Gated Ion-Channel Polypeptide with an Unusual Amino-Terminal Extracellular Domain (1994)
Journal Article
Harvey, R. J., Schmitt, B., Hermans-Borgmeyer, I., Gundelfinger, E. D., Betz, H., & Darlison, M. G. (1994). Sequence of a Drosophila Ligand-Gated Ion-Channel Polypeptide with an Unusual Amino-Terminal Extracellular Domain. Journal of Neurochemistry, 62(6), 2480-2483. https://doi.org/10.1046/j.1471-4159.1994.62062480.x

Abstract: We report the isolation of a full-length clone from a Drosophila melanogaster head cDNA library that encodes a 614-residue polypeptide that exhibits all of the features of a ligand-gated chloride-channel/receptor subunit. This polypeptide,... Read More about Sequence of a Drosophila Ligand-Gated Ion-Channel Polypeptide with an Unusual Amino-Terminal Extracellular Domain.

Alternative Splicing of a 51-Nucleotide Exon that Encodes a Putative Protein Kinase C Phosphorylation Site Generates Two Forms of the Chicken γ-Aminobutyric AcidAReceptor β2 Subunit (1994)
Journal Article
Harvey, R. J., Chinchetru, M. A., & Darlison, M. G. (1994). Alternative Splicing of a 51-Nucleotide Exon that Encodes a Putative Protein Kinase C Phosphorylation Site Generates Two Forms of the Chicken γ-Aminobutyric AcidAReceptor β2 Subunit. Journal of Neurochemistry, 62(1), 10-16. https://doi.org/10.1046/j.1471-4159.1994.62010010.x

Complementary DNAs that encode two forms of the chicken -γ-aminobutyric acid type A (GABAA) receptor β2 subunit have been isolated. These polypeptides differ by the presence (β2L) or absence (β2S) of 17 amino acids, which contain a possible target fo... Read More about Alternative Splicing of a 51-Nucleotide Exon that Encodes a Putative Protein Kinase C Phosphorylation Site Generates Two Forms of the Chicken γ-Aminobutyric AcidAReceptor β2 Subunit.

Sequence and Novel Distribution of the Chicken Homologue of the Mammalianγ-Aminobutyric AcidAReceptorγ1 Subunit (1993)
Journal Article
Glencorse, T. A., Darlison, M. G., Barnard, E. A., & Bateson, A. N. (1993). Sequence and Novel Distribution of the Chicken Homologue of the Mammalianγ-Aminobutyric AcidAReceptorγ1 Subunit. Journal of Neurochemistry, 61(6), 2294-2302. https://doi.org/10.1111/j.1471-4159.1993.tb07473.x

cDNAs have been cloned that encode the chicken (Gallus domesticus) γ-aminobutyric acidA receptor γ1 subunit, the mature sequence of which shares 90, 79, and 69% identity with those of the rat γ1, γ2., and γ3 subunits, respectively. In situ hybridizat... Read More about Sequence and Novel Distribution of the Chicken Homologue of the Mammalianγ-Aminobutyric AcidAReceptorγ1 Subunit.

Molluscan ligand-gated ion-channel receptors (1993)
Book Chapter
Darlison, M. G., Hutton, M. L., & Harvey, R. J. (1993). Molluscan ligand-gated ion-channel receptors. In Comparative Molecular Neurobiology, 48-64. Springer Verlag. https://doi.org/10.1007/978-3-0348-7265-2_3

In this chapter we introduce the reader to the structures of the different types of ligand-gated ion-channel receptor, and the numerous receptor subtypes that have recently been revealed to exist, in both invertebrate and vertebrate species, by the a... Read More about Molluscan ligand-gated ion-channel receptors.

External stimuli and intracellular signalling in the modification of the nematode surface during transition to the mammalian host environment (1993)
Journal Article
Proudfoot, L., Kusel, J. R., Smith, H. V., & Kennedy, M. W. (1993). External stimuli and intracellular signalling in the modification of the nematode surface during transition to the mammalian host environment. Parasitology, 107(05), 559. https://doi.org/10.1017/s0031182000068141

Previous work has shown that the surface of infective larvae of parasitic nematodes will not bind the fluorescent lipid analogue 5-N(octadecanoyl)aminofluorescein (AF18) until after exposure of the parasite to mammalian tissue-culture conditions. In... Read More about External stimuli and intracellular signalling in the modification of the nematode surface during transition to the mammalian host environment.

Molecular cloning reveals the existence of a fourth γ subunit of the vertebrate brain GABAAreceptor (1993)
Journal Article
Harvey, R. J., Kim, H., & Darlison, M. G. (1993). Molecular cloning reveals the existence of a fourth γ subunit of the vertebrate brain GABAAreceptor. FEBS Letters, 331(3), 211-216. https://doi.org/10.1016/0014-5793%2893%2980339-v

We have isolated a cDNA, from the chicken, that encodes a fourth type of γ subunit of the vertebrate brain GABAA receptor. The mature polypeptide (which we name γ4) displays 67%, 69% and 70% identity, respectively, to the rat γ1, γ2 and γ3 subunits.... Read More about Molecular cloning reveals the existence of a fourth γ subunit of the vertebrate brain GABAAreceptor.