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Rheological spectroscopy of concentrated colloidal dispersions (1993)
Journal Article
Williams, P., Williams, D., & Williams, R. (1993). Rheological spectroscopy of concentrated colloidal dispersions. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 77(1), 75-79. https://doi.org/10.1016/0927-7757%2893%2980101-j

A new instrument, the virtual gap rheometer (VGR) for the determination of dynamic shear moduli (and mechanical relaxation spectra therefrom) at frequencies above 100 Hz is described. Several features of the VGR which make it particularly suited to t... Read More about Rheological spectroscopy of concentrated colloidal dispersions.

Rapid changes in the surface of parasitic nematodes during transition from pre- to post-parasitic forms (1993)
Journal Article
Proudfoot, L., Kusel, J. R., Smith, H. V., Harnett, W., Worms, M. J., & Kennedy, M. W. (1993). Rapid changes in the surface of parasitic nematodes during transition from pre- to post-parasitic forms. Parasitology, 107(01), 107. https://doi.org/10.1017/s0031182000079464

All mammalian-parasitic stages of a range of nematode species investigated (Brugia pahangi, Acanthocheilonema viteae, Strongyloides ratti, Nippostrongylus brasiliensis, Trichinella spiralis and Ostertagia ostertagi) labelled in a surface-restricted m... Read More about Rapid changes in the surface of parasitic nematodes during transition from pre- to post-parasitic forms.

A novel invertebrate GABAAreceptor-like polypeptide Sequence and pattern of gene expression (1993)
Journal Article
Hutton, M. L., Harvey, R. J., Earley, F. G., Barnard, E. A., & Darlison, M. G. (1993). A novel invertebrate GABAAreceptor-like polypeptide Sequence and pattern of gene expression. FEBS Letters, 326(1-3), 112-116. https://doi.org/10.1016/0014-5793%2893%2981773-s

A full‐length complementary DNA has been isolated, from the fresh‐water mollusc Lymnaea stagnalis, that encodes a polypeptide (which we have named Ç) that exhibits between 30% and 40% identity to vertebrate GABAA and glycine receptor subunit sequence... Read More about A novel invertebrate GABAAreceptor-like polypeptide Sequence and pattern of gene expression.

Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate (1993)
Journal Article
Darlison, M. G., Bermudez, I., Van Minnen, J., Zimmermann, C., Amar, M., Dautzenberg, F. M., Kim, H., Stühmer, T., & Harvey, R. J. (1993). Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate. Netherlands Journal of Zoology, 44(3), 473-485. https://doi.org/10.1163/156854293x00557

Ligand-gated ion channels (also called ionotropic receptors) are a class of receptors which possess an intrinsic ion pore, selective for either cations or anions, that can be opened by the binding of an appropriate neurotransmitter. Ionotropic recept... Read More about Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate.

Invertebrate GABA and glutamate receptors: molecular biology reveals predictable structures but some unusual pharmacologies (1992)
Journal Article
Darlison, M. G. (1992). Invertebrate GABA and glutamate receptors: molecular biology reveals predictable structures but some unusual pharmacologies. Trends in Neurosciences, 15(12), 469-474. https://doi.org/10.1016/0166-2236%2892%2990091-l

Determination of the sequences of invertebrate γ-aminobutyric acid (GABA)-gated and glutamate-gated receptor/ion channels, through the application of recombinant {DNA} methods, is not just an academic exercise to effect evolutionary comparisons with... Read More about Invertebrate GABA and glutamate receptors: molecular biology reveals predictable structures but some unusual pharmacologies.

The Isolation of Genomic DNA from Invertebrates (1992)
Book Chapter
Vreugdenhil, E., & Darlison, M. G. (1992). The Isolation of Genomic DNA from Invertebrates. In A. Longstaff, & P. Revest (Eds.), Protocols in Molecular Neurobiology, 15-24. Springer Verlag. https://doi.org/10.1385/0-89603-199-3%3A15

Generally, genomic DNA is used either for the construction of genomic libraries or for Southern blot analysis. For several reasons, it is frequently preferable in the field of invertebrate neurobiology to screen, at least initially, genomic rather th... Read More about The Isolation of Genomic DNA from Invertebrates.

GABAA receptor subunit genes as candidate genes for bipolar affective disorder—an association analysis (1992)
Journal Article
Walsh, C., Hicks, A. A., Sham, P., Castle, D., Hunt, N., Clements, A., Sander, T., Murray, R., Darlison, M. G., & Gill, M. (1992). GABAA receptor subunit genes as candidate genes for bipolar affective disorder—an association analysis. Psychiatric Genetics, 2(4), 239-248. https://doi.org/10.1097/00041444-199210000-00002

Polymorphic DNA segments can be used as genetic markers to test for allelic association between a disease and a candidate gene locus. Here we report the results of a study of 60 unrelated bipolar patients in which we sought for evidence of associatio... Read More about GABAA receptor subunit genes as candidate genes for bipolar affective disorder—an association analysis.

Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAAreceptor (1992)
Journal Article
Zaman, S. H., Harvey, R. J., Barnard, E. A., & Darlison, M. G. (1992). Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAAreceptor. FEBS Letters, 307(3), 351-354. https://doi.org/10.1016/0014-5793%2892%2980711-o

We have previously reported [(1991) EMBO J. 10, 3239–3245] the sequence of an invertebrate γ‐aminobutyric acid (GABA) type A (GABAA) receptor polypeptide which forms homo‐oligomeric GABA‐gated, bicuculline‐sensitive, chloride‐ion channels upon hetero... Read More about Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAAreceptor.

Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel (1992)
Journal Article
Traupe, H., Ouweland, A. M. V. D., Oost, B. A. V., Vogel, W., Vetter, U., Warren, S. T., Rocchi, M., Darlison, M. G., & Ropers, H. (1992). Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel. Genomics, 13(2), 481-483. https://doi.org/10.1016/0888-7543%2892%2990279-2

Human biglycan is a small proteoglycan that is expressed at high levels in the growing skeleton and in human skin at the cell surface of differentiating keratinocytes. The human gene for biglycan (BGN) has previously been mapped by in situ hybridizat... Read More about Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel.

Effects of subunit types of the recombinant GABAA receptor on the response to a neurosteroid (1992)
Journal Article
Zaman, S. H., Shingai, R., Harvey, R. J., Darlison, M. G., & Barnard, E. A. (1992). Effects of subunit types of the recombinant GABAA receptor on the response to a neurosteroid. European journal of pharmacology. Molecular pharmacology section, 225(4), 321-330. https://doi.org/10.1016/0922-4106%2892%2990106-6

When vertebrate brain poly(A)+ RNA is expressed in Xenopus oocytes the response of the GABA receptors formed is found to be inhibited allosterically by a neurosteroid, pregnenolone sulphate (PS). This negative modulation was reproduced after expressi... Read More about Effects of subunit types of the recombinant GABAA receptor on the response to a neurosteroid.

Differential Localization of Two Alternatively Spliced GABAAReceptor γ2-Subunit mRNAs in the Chick Brain (1992)
Journal Article
Glencorse, T. A., Bateson, A. N., & Darlison, M. G. (1992). Differential Localization of Two Alternatively Spliced GABAAReceptor γ2-Subunit mRNAs in the Chick Brain. European Journal of Neuroscience, 4(3), 271-277. https://doi.org/10.1111/j.1460-9568.1992.tb00874.x

Two variants of the GABAA receptor γ2 subunit are known to exist, which differ by the presence (γ2L) or absence (γ2S) of eight amino acids in the presumed intracellular loop between the third and fourth membrane-spanning domains. These variants have... Read More about Differential Localization of Two Alternatively Spliced GABAAReceptor γ2-Subunit mRNAs in the Chick Brain.

Cloning of a cDNA that encodes an invertebrate glutamate receptor subunit (1991)
Journal Article
Hutton, M. L., Harvey, R. J., Barnard, E. A., & Darlison, M. (1991). Cloning of a cDNA that encodes an invertebrate glutamate receptor subunit. FEBS Letters, 292(1-2), 111-114. https://doi.org/10.1016/0014-5793%2891%2980846-u

A full‐length cDNA which encodes a putative glutamate receptor polypeptide was isolated from the pond snailLymnaea stagnalis, using a short stretch of exonic sequence and two variants of the polymerase chain reaction. In this first comparison of inve... Read More about Cloning of a cDNA that encodes an invertebrate glutamate receptor subunit.

Conserved Organization of ?-Aminobutyric AcidAReceptor Genes: Cloning and Analysis of the Chicken ?4-Subunit Gene (1991)
Journal Article
Lasham, A., Vreugdenhil, E., Bateson, A. N., Barnard, E. A., & Darlison, M. G. (1991). Conserved Organization of ?-Aminobutyric AcidAReceptor Genes: Cloning and Analysis of the Chicken ?4-Subunit Gene. Journal of Neurochemistry, 57(1), 352-355. https://doi.org/10.1111/j.1471-4159.1991.tb02135.x

Abstract: A series of genomic clones containing DNA that encodes the chicken γ-aminobutyric acidA (GABAA) receptor β4 subunit have been isolated. These have been restriction mapped and partially sequcnced to determine the structural organization and... Read More about Conserved Organization of ?-Aminobutyric AcidAReceptor Genes: Cloning and Analysis of the Chicken ?4-Subunit Gene.

Genetic mapping of the mouse X chromosome in the region homologous to human Xq27–Xq28 (1991)
Journal Article
Herman, G. E., Faust, C. J., Darlison, M. G., & Barnard, E. A. (1991). Genetic mapping of the mouse X chromosome in the region homologous to human Xq27–Xq28. Genomics, 9(4), 670-677. https://doi.org/10.1016/0888-7543%2891%2990360-q

The four loci Gabra3, DXPas8, CamL1, and Bpa, located near the murine X-linked visual pigment gene (Rsup), have been ordered using 248 backcross progeny from an interspecific mating of (B6CBA-Aw-JA-Bpa) and Mus spretus. One hundred twenty backcross p... Read More about Genetic mapping of the mouse X chromosome in the region homologous to human Xq27–Xq28.

y-Aminobutyric AcidA Receptor Heterogeneity Is Increased by Alternative Splicing of a Novel ?-Subunit Gene Transcript (1991)
Journal Article
Bateson, A. N., Lasham, A., & Darlison, M. G. (1991). y-Aminobutyric AcidA Receptor Heterogeneity Is Increased by Alternative Splicing of a Novel ?-Subunit Gene Transcript. Journal of Neurochemistry, 56(4), 1437-1440. https://doi.org/10.1111/j.1471-4159.1991.tb11443.x

Abstract: DNA sequences encoding two variants of a novel γ-aminobutyric acidA (GABAA) receptor β subunit were isolated from an embryonic chicken whole-brain cDNA library and a chicken genomic library. The coding regions of these variants only differ... Read More about y-Aminobutyric AcidA Receptor Heterogeneity Is Increased by Alternative Splicing of a Novel ?-Subunit Gene Transcript.

The chicken GABAA receptor α1 subunit: cDNA sequence and localization of the corresponding mRNA (1991)
Journal Article
Bateson, A. N., Harvey, R. J., Wisden, W., Glencorse, T. A., Hicks, A. A., Hunt, S. P., Barnard, E. A., & Darlison, M. G. (1991). The chicken GABAA receptor α1 subunit: cDNA sequence and localization of the corresponding mRNA. Molecular Brain Research, 9(4), 333-339. https://doi.org/10.1016/0169-328x%2891%2990081-8

We report the sequence of a complementary {DNA} (cDNA) that encodes the chicken {GABAA} receptor α1 subunit, which is extremely homologous to mammalian α1 subunits. The distribution of α1 subunit transcripts is shown to correlate mainly, but not comp... Read More about The chicken GABAA receptor α1 subunit: cDNA sequence and localization of the corresponding mRNA.

Amino acid receptors from insect muscle: electrophysiological characterization in Xenopus oocytes following expression by injection of mRNA (1990)
Journal Article
Fraser, S., Djamgoz, M., Usherwood, P., O'Brien, J., Darlison, M., & Barnard, E. (1990). Amino acid receptors from insect muscle: electrophysiological characterization in Xenopus oocytes following expression by injection of mRNA. Molecular Brain Research, 8(4), 331-341. https://doi.org/10.1016/0169-328x%2890%2990047-h

Poly(A)+ messenger ribonucleic acid (mRNA) was extracted from leg muscles of the locust Schistocerca gregaria and injected into oocytes of Xenopus laevis. After 5–10 days incubation, receptors for l-glutamate, l-quisqualate, dl-ibotenate and γ-aminob... Read More about Amino acid receptors from insect muscle: electrophysiological characterization in Xenopus oocytes following expression by injection of mRNA.