Robert J. Harvey
Sequence of a Drosophila Ligand-Gated Ion-Channel Polypeptide with an Unusual Amino-Terminal Extracellular Domain
Harvey, Robert J.; Schmitt, Bertram; Hermans-Borgmeyer, Irm; Gundelfinger, Eckart D.; Betz, Heinrich; Darlison, Mark G.
Authors
Bertram Schmitt
Irm Hermans-Borgmeyer
Eckart D. Gundelfinger
Heinrich Betz
Mark G. Darlison
Abstract
Abstract: We report the isolation of a full-length clone from a Drosophila melanogaster head cDNA library that encodes a 614-residue polypeptide that exhibits all of the features of a ligand-gated chloride-channel/receptor subunit. This polypeptide, which has been named GRD (denoting that the polypeptide is a GABAA and glycine receptor-like subunit of Drosophila), displays between 33 and 44% identity to vertebrate GABAA and glycine receptor subunits and 32–37% identity to the GABAA receptor-like polypeptides from Drosophila and Lymnaea. It is interesting that the large amino-terminal, presumed extracellular domain of the GRD protein contains an insertion, between the dicysteine loop and the first putative membrane-spanning domain, of 75 amino acids that is not found in any other ligand-gated chloride-channel subunit. Analysis of cDNA and genomic DMA reveals that these residues are encoded by an extension of an exon that is equivalent to exon 6 of vertebrate GABAA and glycine receptor genes. The gene (named Grd) that encodes the Drosophila polypeptide has been mapped, by in situ hybridization, to position 75A on the left arm of chromosome 3.
Citation
Harvey, R. J., Schmitt, B., Hermans-Borgmeyer, I., Gundelfinger, E. D., Betz, H., & Darlison, M. G. (1994). Sequence of a Drosophila Ligand-Gated Ion-Channel Polypeptide with an Unusual Amino-Terminal Extracellular Domain. Journal of Neurochemistry, 62(6), 2480-2483. https://doi.org/10.1046/j.1471-4159.1994.62062480.x
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 18, 1994 |
Publication Date | Jun 30, 1994 |
Deposit Date | Jul 27, 2016 |
Journal | Journal of Neurochemistry |
Print ISSN | 0022-3042 |
Electronic ISSN | 1471-4159 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
Volume | 62 |
Issue | 6 |
Pages | 2480-2483 |
DOI | https://doi.org/10.1046/j.1471-4159.1994.62062480.x |
Keywords | cDNA cloning, Chloride channel, Drosophila melanogaster, gene localization, Grd gene,ligand‐gated ion channel, |
Public URL | http://researchrepository.napier.ac.uk/Output/317679 |
Publisher URL | http://dx.doi.org/10.1046/j.1471-4159.1994.62062480.x |
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