Isolation and sequence analysis of the chicken GABAa receptor α1-subunit gene promoter

Bateson, Alan N.; Ultsch, Andreas; Darlison, Mark G.

Abstract

A genomic clone containing the 5'-flanking sequence of the chicken {GABAA} receptor α1-subunit-encoding gene (GabRα1) was isolated and characterized. An intron was found to interrupt the 5'-untranslated region. The transcription start point (tsp) was determined by primer extension, {RNase} protection and the amplification of chick brain first-strand cDNA. {DNA} sequence analysis revealed a number of putative transcriptional regulatory motifs, including a {TATA} box 30 nucleotides upstream from the tsp, and that this region is a CpG island. While there is conservation between the chicken and human GabRα1 sequences, the chicken GabRα1 promoter has a different structure to those reported for the {GABAA} receptor β3- and δ-subunit-encoding genes.

Citation

Bateson, A. N., Ultsch, A., & Darlison, M. G. (1995). Isolation and sequence analysis of the chicken GABAa receptor α1-subunit gene promoter. Gene, 153(2), 243-247. https://doi.org/10.1016/0378-1119%2894%2900776-o

• Journal Article Type: Article
• Publication Date: 1995-02
• Deposit Date: Jul 27, 2016
• Journal: Gene
• Print ISSN: 0378-1119
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 153
• Issue: 2
• Pages: 243-247
• DOI: https://doi.org/10.1016/0378-1119%2894%2900776-o
• Public URL: http://researchrepository.napier.ac.uk/Output/317691
• Publisher URL: http://www.sciencedirect.com/science/article/pii/037811199400776O