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Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes

Albrecht, Barbara E.; Darlison, Mark G.

Authors

Barbara E. Albrecht

Mark G. Darlison



Abstract

We have amplified partial complementary {DNAs} for the chicken γ-aminobutyric acid type C (GABAC) receptor ρ1 and ρ2 subunits using the polymerase chain reaction. These nucleotide sequences have been utilized to design specific oligonucleotide probes for the in situ hybridization localization of the corresponding messenger {RNAs} (mRNAs) in the 1-day-old chick retina. Although both transcripts are found almost exclusively in the inner nuclear layer, their distributions differ markedly. From the locations of the hybridization signals, we deduce that the ρ1-subunit mRNA is present mainly in bipolar cells and that the ρ2-subunit mRNA is present in both amacrine and horizontal cells. These results suggest that the ρ1 and ρ2 subunits frequently occur in different receptor complexes and, therefore, that subtypes of the {GABAC} receptor exist.

Citation

Albrecht, B. E., & Darlison, M. G. (1995). Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes. Neuroscience Letters, 189(3), 155-158. https://doi.org/10.1016/0304-3940%2895%2911479-g

Journal Article Type Article
Acceptance Date Mar 17, 1995
Publication Date Apr 21, 1995
Deposit Date Jul 27, 2016
Journal Neuroscience Letters
Print ISSN 0304-3940
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 189
Issue 3
Pages 155-158
DOI https://doi.org/10.1016/0304-3940%2895%2911479-g
Keywords General Neuroscience
Public URL http://researchrepository.napier.ac.uk/Output/317695
Publisher URL http://www.sciencedirect.com/science/article/pii/030439409511479G






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