Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells

Bahn, Sabine; Harvey, Robert J.; Darlison, Mark G.; Wisden, William

Abstract

The γ-aminobutyric acid type A receptor cDNAs encoding the α6 subunit homologues from chicken and goldfish have been cloned and sequenced. These proteins exhibit 83 and 75% identity, respectively, to the rat α6 polypeptide. In situ hybridization has demonstrated that, as in mammals, the avian and teleost fish α6 subunit genes are predominately expressed in cerebellar granule cells. Correspondingly, flunitrazepam-nondisplaceable binding of [3H]Ro 15-4513 (a benzodiazepine partial inverse agonist), which is a major characteristic of γ-aminobutyric acid type A receptors that contain the α6 polypeptide, is also mainly found for cerebellar granule cells of fish and chick. The conservation of this expression pattern suggests that γ-aminobutyric acid type A receptors possessing the α6 subunit are of fundamental importance for cerebellar function and that the corresponding gene regulatory elements, e.g., granule cell-specific enhancers, have also been conserved.

Citation

Bahn, S., Harvey, R. J., Darlison, M. G., & Wisden, W. (1996). Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells. Journal of Neurochemistry, 66(5), 1810-1818. https://doi.org/10.1046/j.1471-4159.1996.66051810.x

• Journal Article Type: Article
• Acceptance Date: Dec 21, 1995
• Publication Date: 1996-05
• Deposit Date: Jul 27, 2016
• Journal: Journal of Neurochemistry
• Print ISSN: 0022-3042
• Electronic ISSN: 1471-4159
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 66
• Issue: 5
• Pages: 1810-1818
• DOI: https://doi.org/10.1046/j.1471-4159.1996.66051810.x
• Keywords: Teleost fish; Bird; Granule cell; Benzodiazepine; Cerebellum
• Public URL: http://researchrepository.napier.ac.uk/Output/317698
• Publisher URL: http://dx.doi.org/10.1046/j.1471-4159.1996.66051810.x