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Sequence of a Drosophila Ligand-Gated Ion-Channel Polypeptide with an Unusual Amino-Terminal Extracellular Domain (1994)
Journal Article
Harvey, R. J., Schmitt, B., Hermans-Borgmeyer, I., Gundelfinger, E. D., Betz, H., & Darlison, M. G. (1994). Sequence of a Drosophila Ligand-Gated Ion-Channel Polypeptide with an Unusual Amino-Terminal Extracellular Domain. Journal of Neurochemistry, 62(6), 2480-2483. https://doi.org/10.1046/j.1471-4159.1994.62062480.x

Abstract: We report the isolation of a full-length clone from a Drosophila melanogaster head cDNA library that encodes a 614-residue polypeptide that exhibits all of the features of a ligand-gated chloride-channel/receptor subunit. This polypeptide,... Read More about Sequence of a Drosophila Ligand-Gated Ion-Channel Polypeptide with an Unusual Amino-Terminal Extracellular Domain.

Differential Localization of Two Alternatively Spliced GABAAReceptor γ2-Subunit mRNAs in the Chick Brain (1992)
Journal Article
Glencorse, T. A., Bateson, A. N., & Darlison, M. G. (1992). Differential Localization of Two Alternatively Spliced GABAAReceptor γ2-Subunit mRNAs in the Chick Brain. European Journal of Neuroscience, 4(3), 271-277. https://doi.org/10.1111/j.1460-9568.1992.tb00874.x

Two variants of the GABAA receptor γ2 subunit are known to exist, which differ by the presence (γ2L) or absence (γ2S) of eight amino acids in the presumed intracellular loop between the third and fourth membrane-spanning domains. These variants have... Read More about Differential Localization of Two Alternatively Spliced GABAAReceptor γ2-Subunit mRNAs in the Chick Brain.

Effects of subunit types of the recombinant GABAA receptor on the response to a neurosteroid (1992)
Journal Article
Zaman, S. H., Shingai, R., Harvey, R. J., Darlison, M. G., & Barnard, E. A. (1992). Effects of subunit types of the recombinant GABAA receptor on the response to a neurosteroid. European journal of pharmacology. Molecular pharmacology section, 225(4), 321-330. https://doi.org/10.1016/0922-4106%2892%2990106-6

When vertebrate brain poly(A)+ RNA is expressed in Xenopus oocytes the response of the GABA receptors formed is found to be inhibited allosterically by a neurosteroid, pregnenolone sulphate (PS). This negative modulation was reproduced after expressi... Read More about Effects of subunit types of the recombinant GABAA receptor on the response to a neurosteroid.

Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel (1992)
Journal Article
Traupe, H., Ouweland, A. M. V. D., Oost, B. A. V., Vogel, W., Vetter, U., Warren, S. T., Rocchi, M., Darlison, M. G., & Ropers, H. (1992). Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel. Genomics, 13(2), 481-483. https://doi.org/10.1016/0888-7543%2892%2990279-2

Human biglycan is a small proteoglycan that is expressed at high levels in the growing skeleton and in human skin at the cell surface of differentiating keratinocytes. The human gene for biglycan (BGN) has previously been mapped by in situ hybridizat... Read More about Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel.

Developmental up-regulation and agonist-dependent down-regulation of GABAA receptor subunit mRNAs in chick cortical neurons (1994)
Journal Article
Barnes Jr., E. M., Baumgartner, B. J., Harvey, R. J., & Darlison, M. G. (1994). Developmental up-regulation and agonist-dependent down-regulation of GABAA receptor subunit mRNAs in chick cortical neurons. Molecular Brain Research, 26(1-2), 9-17. https://doi.org/10.1016/0169-328x%2894%2990068-x

Research report We have used quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to analyze the expression of {GABAA} receptor subunit genes in cultured neurons from the chick embryo cerebral cortex. During maturation of the neuro... Read More about Developmental up-regulation and agonist-dependent down-regulation of GABAA receptor subunit mRNAs in chick cortical neurons.

Mental health advance statements: crossing the divide from clinical to law enforcement settings (2020)
Journal Article
Stavert, J. (2021). Mental health advance statements: crossing the divide from clinical to law enforcement settings. Journal of Psychiatric and Mental Health Nursing, 28(1), 102-106. https://doi.org/10.1111/jpm.12695

It is clear that police in many jurisdictions are increasingly being called out to situations involving persons with mental distress. In Scotland, for example, notifications of Place of Safety Orders to the Mental Welfare Commission for Scotland incr... Read More about Mental health advance statements: crossing the divide from clinical to law enforcement settings.

Isolation and sequence analysis of the chicken GABAa receptor α1-subunit gene promoter (1995)
Journal Article
Bateson, A. N., Ultsch, A., & Darlison, M. G. (1995). Isolation and sequence analysis of the chicken GABAa receptor α1-subunit gene promoter. Gene, 153(2), 243-247. https://doi.org/10.1016/0378-1119%2894%2900776-o

A genomic clone containing the 5'-flanking sequence of the chicken {GABAA} receptor α1-subunit-encoding gene (GabRα1) was isolated and characterized. An intron was found to interrupt the 5'-untranslated region. The transcription start point (tsp) was... Read More about Isolation and sequence analysis of the chicken GABAa receptor α1-subunit gene promoter.

Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes (1995)
Journal Article
Albrecht, B. E., & Darlison, M. G. (1995). Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes. Neuroscience Letters, 189(3), 155-158. https://doi.org/10.1016/0304-3940%2895%2911479-g

We have amplified partial complementary {DNAs} for the chicken γ-aminobutyric acid type C (GABAC) receptor ρ1 and ρ2 subunits using the polymerase chain reaction. These nucleotide sequences have been utilized to design specific oligonucleotide probes... Read More about Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes.

Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells (1996)
Journal Article
Bahn, S., Harvey, R. J., Darlison, M. G., & Wisden, W. (1996). Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells. Journal of Neurochemistry, 66(5), 1810-1818. https://doi.org/10.1046/j.1471-4159.1996.66051810.x

The γ-aminobutyric acid type A receptor cDNAs encoding the α6 subunit homologues from chicken and goldfish have been cloned and sequenced. These proteins exhibit 83 and 75% identity, respectively, to the rat α6 polypeptide. In situ hybridization has... Read More about Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells.

Exclusion of linkage between idiopathic generalized epilepsies and the GABAA receptor α1 and γ2 subunit gene cluster on chromosome 5 (1996)
Journal Article
Sander, T., Hildmann, T., Janz, D., Wienker, T. F., Bianchi, A., Bauer, G., Sailer, U., Scaramelli, A., Neitzel, H., Schmitz, B., Bailey, M. E., Beck-Mannagetta, G., Johnson, K. J., & Darlison, M. G. (1996). Exclusion of linkage between idiopathic generalized epilepsies and the GABAA receptor α1 and γ2 subunit gene cluster on chromosome 5. Epilepsy Research, 23(3), 235-244. https://doi.org/10.1016/0920-1211%2895%2900098-4

Hereditary factors play a major role in the etiology of idiopathic generalized epilepsies (IGEs). The pivotal function of ionotropic γ-aminobutyric acid type A receptors (GABRs) in inhibitory neurotransmission in the mammalian central nervous system... Read More about Exclusion of linkage between idiopathic generalized epilepsies and the GABAA receptor α1 and γ2 subunit gene cluster on chromosome 5.

Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed inXenopus oocytes from a cloned cDNA (1995)
Journal Article
Bhandal, N. S., Ramsey, R. L., Harvey, R. J., Darlison, M. G., & Usherwood, P. N. R. (1995). Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed inXenopus oocytes from a cloned cDNA. Invertebrate Neuroscience, 1(3), 267-272. https://doi.org/10.1007/bf02211028

We have previously described the isolation of a complementary DNA (cDNA) from the freshwater molluscLymnaea stagnalis encoding a polypeptide that exhibits ∼50{%} identity to the {ß}-subunits of vertebrate $γ$-aminobutyric acid (GABA) type A (GABAA) r... Read More about Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed inXenopus oocytes from a cloned cDNA.

Steroid signalling in the ovarian surface epithelium (2005)
Journal Article
Rae, M. T., & Hillier, S. G. (2005). Steroid signalling in the ovarian surface epithelium. Trends in Endocrinology and Metabolism, 16(7), 327-333. https://doi.org/10.1016/j.tem.2005.07.002

Human ovarian surface epithelium (HOSE) undergoes serial injury-repair with each ovulation, which is probably why most ovarian epithelial cancers arise there. Considering the proposed inflammatory aetiology of ovarian cancer, anti-inflammatory steroi... Read More about Steroid signalling in the ovarian surface epithelium.

Impact of maternal undernutrition and fetal number on glucocorticoid, growth hormone and insulin-like growth factor receptor mRNA abundance in the ovine fetal kidney (2005)
Journal Article
Brennan, K. A., Gopalakrishnan, G. S., Kurlak, L., Rhind, S. M., Kyle, C. E., Brooks, A. N., Rae, M. T., Olson, D. M., Stephenson, T., & Symonds, M. E. (2005). Impact of maternal undernutrition and fetal number on glucocorticoid, growth hormone and insulin-like growth factor receptor mRNA abundance in the ovine fetal kidney. Reproduction, 129, 151-159. https://doi.org/10.1530/rep.1.00229

Epidemiological and animal studies strongly indicate that the environment experienced in utero determines, in part, an individual’s likelihood of developing cardiovascular disease in later life. This risk has been further linked to impaired kidney fu... Read More about Impact of maternal undernutrition and fetal number on glucocorticoid, growth hormone and insulin-like growth factor receptor mRNA abundance in the ovine fetal kidney.

Specific Non-Genomic, Membrane-Localized Binding Sites for Progesterone in the Bovine Corpus Luteum1 (1998)
Journal Article
Rae, M. T., Menzies, G. S., McNeilly, A. S., Woad, K., Webb, R., & Bramley, T. A. (1998). Specific Non-Genomic, Membrane-Localized Binding Sites for Progesterone in the Bovine Corpus Luteum1. Biology of Reproduction, 58(6), 1394-1406. https://doi.org/10.1095/biolreprod58.6.1394

Fractionation of bovine corpus luteum (CL) homogenates on continuous sucrose density gradients with and without preincubation with 3H-progesterone demonstrated high levels of tracer binding and high content of endogenous progesterone associated with... Read More about Specific Non-Genomic, Membrane-Localized Binding Sites for Progesterone in the Bovine Corpus Luteum1.

Fungal interactions with metals and radionuclides for environmental bioremediation (1996)
Book Chapter
Singleton, I., & Tobin, J. M. (1996). Fungal interactions with metals and radionuclides for environmental bioremediation. In J. C. Frankland, N. Magan, & G. M. Gadd (Eds.), Fungi and environmental change (282-298). Cambridge University Press. https://doi.org/10.1017/CBO9780511753190.018

Microorganisms, including fungi, are known to accumulate metals from their external environment and the possibility of using fungi as a means of treating metal/radionuclide-containing effluents is well recognized (Siegel, Galun & Siegel, 1990; Gadd,... Read More about Fungal interactions with metals and radionuclides for environmental bioremediation.

Fungal remediation of soils contaminated with persistent organic pollutants (2001)
Book Chapter
Singleton, I. (2001). Fungal remediation of soils contaminated with persistent organic pollutants. In G. M. Gadd (Ed.), Fungi in Bioremediation, (79-96). Cambridge: Cambridge University Press. https://doi.org/10.1017/CBO9780511541780.005

Laboratory-based studies have shown that fungi are able to degrade a wide range of organic pollutants (see other chapters) and have great potential for use as inoculants to remediate contaminated soil. However, soil is a heterogeneous environment and... Read More about Fungal remediation of soils contaminated with persistent organic pollutants.

NuMA is required for proper spindle assembly and chromosome alignment in prometaphase (2009)
Journal Article
Haren, L., Gnadt, N., Wright, M., & Merdes, A. (2009). NuMA is required for proper spindle assembly and chromosome alignment in prometaphase. BMC Research Notes, 2, Article 64. https://doi.org/10.1186/1756-0500-2-64

NuMA is a protein that has been previously shown to play a role in focusing microtubules at the mitotic spindle poles. However, most previous work relies on experimental methods that might cause dominant side effects on spindle formation, such as mic... Read More about NuMA is required for proper spindle assembly and chromosome alignment in prometaphase.

Differential expression from two iron-responsive promoters in Salmonella enterica serovar Typhimurium reveals the presence of iron in macrophage-phagosomes (2008)
Journal Article
Taylor, C. M., Osman, D., & Cavet, J. S. (2009). Differential expression from two iron-responsive promoters in Salmonella enterica serovar Typhimurium reveals the presence of iron in macrophage-phagosomes. Microbial Pathogenesis, 46(2), 114-118. https://doi.org/10.1016/j.micpath.2008.11.001

The metal status of macrophage-phagosomes during Salmonella infection is largely unknown. In this study, we have precisely calibrated the metal-specificities of two metal-responsive promoters, PiroBCDE and PsodB, from Salmonella enterica serovar Typh... Read More about Differential expression from two iron-responsive promoters in Salmonella enterica serovar Typhimurium reveals the presence of iron in macrophage-phagosomes.

Characterization of relA and codY mutants of Listeria monocytogenes: identification of the CodY regulon and its role in virulence (2007)
Journal Article
Bennett, H. J., Pearce, D. M., Glenn, S., Taylor, C. M., Kuhn, M., Sonenshein, A. L., Andrew, P. W., & Roberts, I. S. (2007). Characterization of relA and codY mutants of Listeria monocytogenes: identification of the CodY regulon and its role in virulence. Molecular Microbiology, 63(5), 1453-1467. https://doi.org/10.1111/j.1365-2958.2007.05597.x

Listeria monocytogenes is a Gram-positive intracellular parasite and the causative organism of human listeriosis. In this article we demonstrate that L. monocytogenes encodes a functional member of the CodY family of global regulatory proteins that i... Read More about Characterization of relA and codY mutants of Listeria monocytogenes: identification of the CodY regulon and its role in virulence.