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Sequence of a Drosophila Ligand-Gated Ion-Channel Polypeptide with an Unusual Amino-Terminal Extracellular Domain (1994)
Journal Article
Harvey, R. J., Schmitt, B., Hermans-Borgmeyer, I., Gundelfinger, E. D., Betz, H., & Darlison, M. G. (1994). Sequence of a Drosophila Ligand-Gated Ion-Channel Polypeptide with an Unusual Amino-Terminal Extracellular Domain. Journal of Neurochemistry, 62(6)

Abstract: We report the isolation of a full-length clone from a Drosophila melanogaster head cDNA library that encodes a 614-residue polypeptide that exhibits all of the features of a ligand-gated chloride-channel/receptor subunit. This polypeptide,... Read More about Sequence of a Drosophila Ligand-Gated Ion-Channel Polypeptide with an Unusual Amino-Terminal Extracellular Domain.

Further Evidence for Clustering of Human GABAA Receptor Subunit Genes: Localization of the α6-Subunit Gene (GABRA6) to Distal Chromosome 5q by Linkage Analysis (1994)
Journal Article
Hicks, A. A., Bailey, M. E., Riley, B. P., Kamphuis, W., Siciliano, M. J., Johnson, K. J., & Darlison, M. G. (1994). Further Evidence for Clustering of Human GABAA Receptor Subunit Genes: Localization of the α6-Subunit Gene (GABRA6) to Distal Chromosome 5q by Linkage Analysis. Genomics, 20(2), 285-288. https://doi.org/10.1006/geno.1994.1167

GABAA receptors are hetero-oligomeric ion-channel complexes that are composed of combinations of α, β, γ, and δ subunits and play a major role in inhibitory neurotransmission in the mammalian brain. We report here a microsatellite polymorphism within... Read More about Further Evidence for Clustering of Human GABAA Receptor Subunit Genes: Localization of the α6-Subunit Gene (GABRA6) to Distal Chromosome 5q by Linkage Analysis.

Alternative Splicing of a 51-Nucleotide Exon that Encodes a Putative Protein Kinase C Phosphorylation Site Generates Two Forms of the Chicken γ-Aminobutyric AcidAReceptor β2 Subunit (1994)
Journal Article
Harvey, R. J., Chinchetru, M. A., & Darlison, M. G. (1994). Alternative Splicing of a 51-Nucleotide Exon that Encodes a Putative Protein Kinase C Phosphorylation Site Generates Two Forms of the Chicken γ-Aminobutyric AcidAReceptor β2 Subunit. Journal of

Complementary DNAs that encode two forms of the chicken -γ-aminobutyric acid type A (GABAA) receptor β2 subunit have been isolated. These polypeptides differ by the presence (β2L) or absence (β2S) of 17 amino acids, which contain a possible target fo... Read More about Alternative Splicing of a 51-Nucleotide Exon that Encodes a Putative Protein Kinase C Phosphorylation Site Generates Two Forms of the Chicken γ-Aminobutyric AcidAReceptor β2 Subunit.

Multiplicity, Structure, and Function in GABAAReceptors (1993)
Journal Article
BARNARD, E. A., SUTHERLAND, M., ZAMAN, S., MATSUMOTO, M., NAYEEM, N., GREEN, T., …BATESON, A. N. (1993). Multiplicity, Structure, and Function in GABAAReceptors. Annals of the New York Academy of Sciences, 707(1 Molecular Bas), 116-125. https://doi.org/

No abstract available.

Molluscan ligand-gated ion-channel receptors (1993)
Book Chapter
Darlison, M. G., Hutton, M. L., & Harvey, R. J. (1993). Molluscan ligand-gated ion-channel receptors. In Comparative Molecular Neurobiology, 48-64. Springer Verlag. https://doi.org/10.1007/978-3-0348-7265-2_3

In this chapter we introduce the reader to the structures of the different types of ligand-gated ion-channel receptor, and the numerous receptor subtypes that have recently been revealed to exist, in both invertebrate and vertebrate species, by the a... Read More about Molluscan ligand-gated ion-channel receptors.

Sequence and Novel Distribution of the Chicken Homologue of the Mammalianγ-Aminobutyric AcidAReceptorγ1 Subunit (1993)
Journal Article
Glencorse, T. A., Darlison, M. G., Barnard, E. A., & Bateson, A. N. (1993). Sequence and Novel Distribution of the Chicken Homologue of the Mammalianγ-Aminobutyric AcidAReceptorγ1 Subunit. Journal of Neurochemistry, 61(6), 2294-2302. https://doi.org/10.

cDNAs have been cloned that encode the chicken (Gallus domesticus) γ-aminobutyric acidA receptor γ1 subunit, the mature sequence of which shares 90, 79, and 69% identity with those of the rat γ1, γ2., and γ3 subunits, respectively. In situ hybridizat... Read More about Sequence and Novel Distribution of the Chicken Homologue of the Mammalianγ-Aminobutyric AcidAReceptorγ1 Subunit.

Molecular cloning reveals the existence of a fourth γ subunit of the vertebrate brain GABAAreceptor (1993)
Journal Article
Harvey, R. J., Kim, H., & Darlison, M. G. (1993). Molecular cloning reveals the existence of a fourth γ subunit of the vertebrate brain GABAAreceptor. FEBS Letters, 331(3), 211-216. https://doi.org/10.1016/0014-5793%2893%2980339-v

We have isolated a cDNA, from the chicken, that encodes a fourth type of γ subunit of the vertebrate brain GABAA receptor. The mature polypeptide (which we name γ4) displays 67%, 69% and 70% identity, respectively, to the rat γ1, γ2 and γ3 subunits.... Read More about Molecular cloning reveals the existence of a fourth γ subunit of the vertebrate brain GABAAreceptor.

A novel invertebrate GABAAreceptor-like polypeptide Sequence and pattern of gene expression (1993)
Journal Article
Hutton, M. L., Harvey, R. J., Earley, F. G., Barnard, E. A., & Darlison, M. G. (1993). A novel invertebrate GABAAreceptor-like polypeptide Sequence and pattern of gene expression. FEBS Letters, 326(1-3), 112-116. https://doi.org/10.1016/0014-5793%2893%298

A full‐length complementary DNA has been isolated, from the fresh‐water mollusc Lymnaea stagnalis, that encodes a polypeptide (which we have named Ç) that exhibits between 30% and 40% identity to vertebrate GABAA and glycine receptor subunit sequence... Read More about A novel invertebrate GABAAreceptor-like polypeptide Sequence and pattern of gene expression.

Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique (1993)
Journal Article
Lasham, A., & Darlison, M. G. (1993). Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique. Molecular and Cellular Probes, 7(1), 67-73. doi:10.1006/mcpr.1993.1009

We described an improved method for directly sequencing lambda (λ) DNA that has been isolated from either crude cleared lysates or plate lysates. This protocol does not require that the DNA be obtained from bacteriophage particles that have been puri... Read More about Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique.

Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate (1993)
Journal Article
Darlison, M. G., Bermudez, I., Van Minnen, J., Zimmermann, C., Amar, M., Dautzenberg, F. M., …Harvey, R. J. (1993). Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric A

Ligand-gated ion channels (also called ionotropic receptors) are a class of receptors which possess an intrinsic ion pore, selective for either cations or anions, that can be opened by the binding of an appropriate neurotransmitter. Ionotropic recept... Read More about Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate.

The Isolation of Genomic DNA from Invertebrates (1992)
Book Chapter
Vreugdenhil, E., & Darlison, M. G. (1992). The Isolation of Genomic DNA from Invertebrates. In A. Longstaff, & P. Revest (Eds.), Protocols in Molecular Neurobiology, 15-24. Springer Verlag. https://doi.org/10.1385/0-89603-199-3%3A15

Generally, genomic DNA is used either for the construction of genomic libraries or for Southern blot analysis. For several reasons, it is frequently preferable in the field of invertebrate neurobiology to screen, at least initially, genomic rather th... Read More about The Isolation of Genomic DNA from Invertebrates.

Invertebrate GABA and glutamate receptors: molecular biology reveals predictable structures but some unusual pharmacologies (1992)
Journal Article
Darlison, M. G. (1992). Invertebrate GABA and glutamate receptors: molecular biology reveals predictable structures but some unusual pharmacologies. Trends in Neurosciences, 15(12), 469-474. https://doi.org/10.1016/0166-2236%2892%2990091-l

Determination of the sequences of invertebrate γ-aminobutyric acid (GABA)-gated and glutamate-gated receptor/ion channels, through the application of recombinant {DNA} methods, is not just an academic exercise to effect evolutionary comparisons with... Read More about Invertebrate GABA and glutamate receptors: molecular biology reveals predictable structures but some unusual pharmacologies.

Confirmation of the localization of the human GABAA receptor α1-subunit gene (GABRA1) to distal 5q by linkage analysis (1992)
Journal Article
Johnson, K. J., Sander, T., Hicks, A. A., van Marle, A., Janz, D., Mullan, M. J., …Darlison, M. G. (1992). Confirmation of the localization of the human GABAA receptor α1-subunit gene (GABRA1) to distal 5q by linkage analysis. Genomics, 14(3), 745-748. https://doi.org/10.1016/s0888-7543%2805%2980178-8

The GABAA receptor is the major inhibitory neurotransmitter receptor in the mammalian brain. To date, 14 genes that encode subunits of this receptor have been identified; these appear to be scattered throughout the human genome and are under investig... Read More about Confirmation of the localization of the human GABAA receptor α1-subunit gene (GABRA1) to distal 5q by linkage analysis.

GABAA receptor subunit genes as candidate genes for bipolar affective disorder—an association analysis (1992)
Journal Article
Walsh, C., Hicks, A. A., Sham, P., Castle, D., Hunt, N., Clements, A., …Gill, M. (1992). GABAA receptor subunit genes as candidate genes for bipolar affective disorder—an association analysis. Psychiatric Genetics, 2(4), 239-248. https://doi.org/10.10

Polymorphic DNA segments can be used as genetic markers to test for allelic association between a disease and a candidate gene locus. Here we report the results of a study of 60 unrelated bipolar patients in which we sought for evidence of associatio... Read More about GABAA receptor subunit genes as candidate genes for bipolar affective disorder—an association analysis.

Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAAreceptor (1992)
Journal Article
Zaman, S. H., Harvey, R. J., Barnard, E. A., & Darlison, M. G. (1992). Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAAreceptor. FEBS Letters, 307(3), 351-354. https://doi.org/10.1016/0014-5793%2892%2980711

We have previously reported [(1991) EMBO J. 10, 3239–3245] the sequence of an invertebrate γ‐aminobutyric acid (GABA) type A (GABAA) receptor polypeptide which forms homo‐oligomeric GABA‐gated, bicuculline‐sensitive, chloride‐ion channels upon hetero... Read More about Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAAreceptor.

Assembly of functional GABAA receptors in insect cells using baculovirus expression vectors (1992)
Journal Article
Atkinson, A. E., Bermudez, I., Darlison, M. G., Barnard, E. A., Earley, F. G. P., Possee, R. D., …King, L. A. (1992). Assembly of functional GABAA receptors in insect cells using baculovirus expression vectors. NeuroReport, 3(7), 597-600. https://doi.or

We have constructed recombinant baculoviruses containing cDNAs encoding either the [alpha]1- or the [beta]1-subunit of the bovine GABAA receptor. In Spodoptera frugiperda (IPLB-Sf-21) cells infected with recombinant virus expressing either the [alpha... Read More about Assembly of functional GABAA receptors in insect cells using baculovirus expression vectors.

Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel (1992)
Journal Article
Traupe, H., Ouweland, A. M. V. D., Oost, B. A. V., Vogel, W., Vetter, U., Warren, S. T., …Ropers, H. (1992). Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel. Genomics, 13(2), 481-483. https://doi.org/10

Human biglycan is a small proteoglycan that is expressed at high levels in the growing skeleton and in human skin at the cell surface of differentiating keratinocytes. The human gene for biglycan (BGN) has previously been mapped by in situ hybridizat... Read More about Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel.

Effects of subunit types of the recombinant GABAA receptor on the response to a neurosteroid (1992)
Journal Article
Zaman, S. H., Shingai, R., Harvey, R. J., Darlison, M. G., & Barnard, E. A. (1992). Effects of subunit types of the recombinant GABAA receptor on the response to a neurosteroid. European journal of pharmacology. Molecular pharmacology section, 225(4), 321

When vertebrate brain poly(A)+ RNA is expressed in Xenopus oocytes the response of the GABA receptors formed is found to be inhibited allosterically by a neurosteroid, pregnenolone sulphate (PS). This negative modulation was reproduced after expressi... Read More about Effects of subunit types of the recombinant GABAA receptor on the response to a neurosteroid.

Differential Localization of Two Alternatively Spliced GABAAReceptor γ2-Subunit mRNAs in the Chick Brain (1992)
Journal Article
Glencorse, T. A., Bateson, A. N., & Darlison, M. G. (1992). Differential Localization of Two Alternatively Spliced GABAAReceptor γ2-Subunit mRNAs in the Chick Brain. European Journal of Neuroscience, 4(3), 271-277. https://doi.org/10.1111/j.1460-9568.199

Two variants of the GABAA receptor γ2 subunit are known to exist, which differ by the presence (γ2L) or absence (γ2S) of eight amino acids in the presumed intracellular loop between the third and fourth membrane-spanning domains. These variants have... Read More about Differential Localization of Two Alternatively Spliced GABAAReceptor γ2-Subunit mRNAs in the Chick Brain.