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Prof Ian Singleton's Outputs (59)

A new concept of bio-based prestress technology with experimental Proof-of-Concept on Bamboo-Timber composite beams (2023)
Journal Article
Zhang, H., Shen, M., Deng, Y., Andras, P., Sukontasukkul, P., Yuen, T. Y. P., Wong, S. H., Limkatanyu, S., Singleton, I., & Hansapinyo, C. (2023). A new concept of bio-based prestress technology with experimental Proof-of-Concept on Bamboo-Timber composite beams. Construction and Building Materials, 402, Article 132991. https://doi.org/10.1016/j.conbuildmat.2023.132991

This paper presents a pioneering experimental proof-of-concept study to validate a novel concept of prestress technology that used only pure bio-based composite materials while achieved consistent prestressed stress distribution within the structure... Read More about A new concept of bio-based prestress technology with experimental Proof-of-Concept on Bamboo-Timber composite beams.

Point of (no) return? Vegetation structure and diversity of restored mangroves in Sulawesi, Indonesia, 14–16 years on (2023)
Journal Article
Djamaluddin, R., Fusi, M., Djabar, B., Evans, D., Holmes, R., Huxham, M., O'Connell, D. P., Salzmann, U., Singleton, I., Tjoa, A., Trianto, A., & Diele, K. (2023). Point of (no) return? Vegetation structure and diversity of restored mangroves in Sulawesi, Indonesia, 14–16 years on. Restoration Ecology, 31(7), Article e13963. https://doi.org/10.1111/rec.13963

Mangrove forests, benefitting millions of people, experience significant degradation. Global recognition of the urgency of halting and reversing this trend have initiated numerous restoration activities. Restoration success is typically evaluated by... Read More about Point of (no) return? Vegetation structure and diversity of restored mangroves in Sulawesi, Indonesia, 14–16 years on.

Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent (2023)
Journal Article
Trianto, A., Radjasa, O. K., Subagiyo, S., Isabella, A. A., Bahry, M. S., Purnaweni, H., Djamaludin, R., Tjoa, A., Singleton, I., Evans, D. M., & Diele, K. (2023). Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent. ILMU KELAUTAN: Indonesian Journal of Marine Sciences, 28(2), 136-147. https://doi.org/10.14710/ik.ijms.28.2.136-147

Mangroves are complex, unique, and dynamic ecosystems that host organisms that have special physiological adaptations to fluctuations in salinity, temperature, and pH. Gastropods have an important role in the mangrove ecosystem for food cycles and we... Read More about Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent.

Bacterial communities in soils as indicators of the potential of syenite as an agromineral (2022)
Journal Article
de Carvalho Baptista, J., Duncan Gray, N., Büchler Tarumoto, M., Singleton, I., McCann, C. M., & Manning, D. A. C. (2022). Bacterial communities in soils as indicators of the potential of syenite as an agromineral. Pesquisa Agropecuária Brasileira, 57, Article e01414. https://doi.org/10.1590/S1678-3921.pab2022.v57.01414

The objective of this work was to identify microbial communities associated with the surfaces of alkali feldspars and to determine whether these microbes might be involved in the weathering of these rocks for agronomic benefit. Samples were taken fro... Read More about Bacterial communities in soils as indicators of the potential of syenite as an agromineral.

Top-Down Characterisation of an Antimicrobial Sanitiser, Leading from Quenchers of Efficacy to Mode of Action (2020)
Journal Article
Wohlgemuth, F., Gomes, R. L., Singleton, I., Rawson, F. J., & Avery, S. V. (2020). Top-Down Characterisation of an Antimicrobial Sanitiser, Leading from Quenchers of Efficacy to Mode of Action. Frontiers in Microbiology, 11, Article 575157. https://doi.org/10.3389/fmicb.2020.575157

We developed a top-down strategy to characterise an antimicrobial, oxidising sanitiser, which has diverse proposed applications including surface-sanitisation of fresh foods, and with benefits for water resilience. The strategy involved finding quenc... Read More about Top-Down Characterisation of an Antimicrobial Sanitiser, Leading from Quenchers of Efficacy to Mode of Action.

The fungal threat to global food security (2019)
Journal Article
Avery, S. V., Singleton, I., Magan, N., & Goldman, G. H. (2019). The fungal threat to global food security. Fungal Biology, 123(8), 555-557. https://doi.org/10.1016/j.funbio.2019.03.006

Fungi threaten the security of food supply to human populations on several fronts. They destroy up to 30 % of crop products through disease and spoilage processes, while mycotoxin-producing fungi and opportunistic pathogens endanger food safety. Cont... Read More about The fungal threat to global food security.

Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential. (2019)
Journal Article
Smith, A., Hearn, J., Taylor, C., Wheelhouse, N., Kaczmarek, M., Moorhouse, E., & Singleton, I. (2019). Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential. International Journal of Food Microbiology, 299, 23-32. https://doi.org/10.1016/j.ijfoodmicro.2019.03.013

Listeria monocytogenes is sporadically detected on a range of ready to eat fresh produce lines, such as spinach and rocket, and is a threat to public health. However, little is known about the diversity of L. monocytogenes present on fresh produce an... Read More about Listeria monocytogenes isolates from ready to eat plant produce are diverse and have virulence potential..

Microbes associated with fresh produce: sources, types and methods to reduce spoilage and contamination (2019)
Book Chapter
Kaczmarek, M., Avery, S. V., & Singleton, I. (2019). Microbes associated with fresh produce: sources, types and methods to reduce spoilage and contamination. In G. M. Gadd, & S. Sariaslani (Eds.), Advances in Applied Microbiology. Vol 107 (29-82). Elsevier. https://doi.org/10.1016/bs.aambs.2019.02.001

Global food security remains one of the most important challenges that needs to be addressed to ensure the increasing demand for food of the fast growing human population is satisfied. Fruits and vegetables comprise an essential component of a health... Read More about Microbes associated with fresh produce: sources, types and methods to reduce spoilage and contamination.

Sources and survival of Listeria monocytogenes on fresh, leafy produce (2018)
Journal Article
on fresh, leafy produce. Journal of Applied Microbiology, 125(4), 930-942. https://doi.org/10.1111/jam.14025

Listeria monocytogenes in an intracellular human pathogen which enters the body through contaminated food stuffs and is known to contaminate fresh leafy produce such as spinach, lettuce and rocket. Routinely, fresh leafy produce is grown and processe... Read More about Sources and survival of Listeria monocytogenes on fresh, leafy produce.

Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review (2018)
Journal Article
Alegbeleye, O. O., Singleton, I., & Sant’Ana, A. S. (2018). Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review. Food Microbiology, 73, 177-208. https://doi.org/10.1016/j.fm.2018.01.003

Foodborne illness resulting from the consumption of contaminated fresh produce is a common phenomenon and has severe effects on human health together with severe economic and social impacts. The implications of foodborne diseases associated with fres... Read More about Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review.

Evidence for rapid, tide-related shifts in the microbiome of the coral Coelastrea aspera (2017)
Journal Article
Sweet, M. J., Brown, B. E., Dunne, R. P., Singleton, I., & Bulling, M. (2017). Evidence for rapid, tide-related shifts in the microbiome of the coral Coelastrea aspera. Coral Reefs, 36(3), 815-828. https://doi.org/10.1007/s00338-017-1572-y

Shifts in the microbiome of the intertidal coral Coelastrea aspera (formally known as Goniastrea aspera) from Phuket, Thailand were noted over the course of a four day period of spring tides. During this time corals were naturally exposed to high tem... Read More about Evidence for rapid, tide-related shifts in the microbiome of the coral Coelastrea aspera.

Isolation of Indigenous Hydrocarbon Transforming Bacteria from Oil Contaminated Soils in Libya: Selection for Use as Potential Inocula for Soil Bioremediation (2017)
Journal Article
Hakima, A., & Singleton, I. (2017). Isolation of Indigenous Hydrocarbon Transforming Bacteria from Oil Contaminated Soils in Libya: Selection for Use as Potential Inocula for Soil Bioremediation. International journal of environmental bioremediation & biodegradation, 5(1), 8-17. https://doi.org/10.12691/ijebb-5-1-2

The Libyan oil industry has left a significant legacy of contamination and methods are required to remediate oil-contaminated soils in the area. In this work hydrocarbon utilizing microorganisms were isolated and identified from contaminated soil sam... Read More about Isolation of Indigenous Hydrocarbon Transforming Bacteria from Oil Contaminated Soils in Libya: Selection for Use as Potential Inocula for Soil Bioremediation.

Soil contamination with silver nanoparticles reduces Bishop pine growth and ectomycorrhizal diversity on pine roots (2015)
Journal Article
Sweet, M. J., & Singleton, I. (2015). Soil contamination with silver nanoparticles reduces Bishop pine growth and ectomycorrhizal diversity on pine roots. Journal of Nanoparticle Research, 17, 448. https://doi.org/10.1007/s11051-015-3246-4

Soil contamination by silver nanoparticles (AgNP) is of potential environmental concern but little work has been carried out on the effect of such contamination on ectomycorrhizal fungi (EMF). EMF are essential to forest ecosystem functions as they a... Read More about Soil contamination with silver nanoparticles reduces Bishop pine growth and ectomycorrhizal diversity on pine roots.

Investigation of potential reasons for bacterial survival on ‘ready-to-eat’ leafy produce during exposure to gaseous ozone (2015)
Journal Article
Wani, S., Barnes, J., & Singleton, I. (2016). Investigation of potential reasons for bacterial survival on ‘ready-to-eat’ leafy produce during exposure to gaseous ozone. Postharvest Biology and Technology, 111, 185-190. https://doi.org/10.1016/j.postharvbio.2015.08.007

Fresh leafy produce, such as lettuce and coriander, are subject to post-harvest microbial contamination and decay. Because of increasing pesticide resistance and consumer pressures, alternative residue-free treatments, such as ozone, are being active... Read More about Investigation of potential reasons for bacterial survival on ‘ready-to-eat’ leafy produce during exposure to gaseous ozone.

Effect of Ozone Treatment on Inactivation of Escherichia coli and Listeria sp. on Spinach (2015)
Journal Article
Wani, S., Maker, J., Thompson, J., Barnes, J., & Singleton, I. (2015). Effect of Ozone Treatment on Inactivation of Escherichia coli and Listeria sp. on Spinach. Agriculture, 5(2), 155-169. https://doi.org/10.3390/agriculture5020155

The efficacy of “gaseous” ozone in reducing numbers and re-growth of food-borne pathogens, (Escherichia coli and Listeria spp.), on leafy salads was investigated using spinach. A preliminary in vivo study showed 1-log reduction in six strains of E. c... Read More about Effect of Ozone Treatment on Inactivation of Escherichia coli and Listeria sp. on Spinach.

Fungi at a Small Scale: Spatial Zonation of Fungal Assemblages around Single Trees (2013)
Journal Article
Branco, S., Bruns, T. D., & Singleton, I. (2013). Fungi at a Small Scale: Spatial Zonation of Fungal Assemblages around Single Trees. PLOS ONE, 8(10), Article e78295. https://doi.org/10.1371/journal.pone.0078295

Biological communities are often structured by environmental factors even at small spatial scales. Fungi are no exception, though the patterns and mechanisms underlying their community structure are usually unknown. Previous work documented zonation... Read More about Fungi at a Small Scale: Spatial Zonation of Fungal Assemblages around Single Trees.

Profiling shifts in protein complement in tomato fruit induced by atmospheric ozone-enrichment and/or wound-inoculation with Botrytis cinerea (2013)
Journal Article
Tzortzakis, N., Taybi, T., Antony, E., Singleton, I., Borland, A., & Barnes, J. (2013). Profiling shifts in protein complement in tomato fruit induced by atmospheric ozone-enrichment and/or wound-inoculation with Botrytis cinerea. Postharvest Biology and Technology, 78, 67-75. https://doi.org/10.1016/j.postharvbio.2012.12.005

To unravel the mechanism by which low level atmospheric ozone-enrichment (0.05 μmol mol−1) increases the shelf-life of tomato fruit (Lycopersicon esculentum Mill.) by suppressing the growth of pathogens (Botrytis cinerea), protein yield and compositi... Read More about Profiling shifts in protein complement in tomato fruit induced by atmospheric ozone-enrichment and/or wound-inoculation with Botrytis cinerea.

Chapter Five - Review: Metal-Based Nanoparticles; Size, Function, and Areas for Advancement in Applied Microbiology (2012)
Book Chapter
Sweet, M. J., Chessher, A., & Singleton, I. (2012). Chapter Five - Review: Metal-Based Nanoparticles; Size, Function, and Areas for Advancement in Applied Microbiology. Advances in Applied Microbiology (113-142). Elsevier. https://doi.org/10.1016/B978-0-12-394381-1.00005-2

Abstract Nanoparticles (NPs) are attracting increased attention in commerce and applied microbiology due to their antimicrobial activity, high electrical conductivity, and optical properties. For example, silver {NPs} have broad spectrum antimicrobia... Read More about Chapter Five - Review: Metal-Based Nanoparticles; Size, Function, and Areas for Advancement in Applied Microbiology.

Avian Urine: Its Potential as a Non-Invasive Biomonitor of Environmental Metal Exposure in Birds (2012)
Journal Article
Clapp, J. B., Bevan, R. M., & Singleton, I. (2012). Avian Urine: Its Potential as a Non-Invasive Biomonitor of Environmental Metal Exposure in Birds. Water, air, and soil pollution, 223(7), 3923-3938. https://doi.org/10.1007/s11270-012-1161-1

Current non-invasive biomonitoring techniques to measure heavy metal exposure in free ranging birds using eggs, feathers and guano are problematic because essential metals copper (Cu) and zinc (Zn) deposited in eggs and feathers are under physiologic... Read More about Avian Urine: Its Potential as a Non-Invasive Biomonitor of Environmental Metal Exposure in Birds.

Silver Nanoparticles: A Microbial Perspective (2011)
Book Chapter
Sweet, M., & Singleton, I. (2011). Silver Nanoparticles: A Microbial Perspective. . Academic Press. https://doi.org/10.1016/B978-0-12-387044-5.00005-4

Abstract Silver nanoparticles (NPs) are used for a wide range of commercial reasons to restrict microbial growth. The increasing use of silver {NPs} in modern materials ensures they will find their way into environmental systems. The mode of action w... Read More about Silver Nanoparticles: A Microbial Perspective.

Low-level atmospheric ozone exposure induces protection against Botrytis cinerea with down-regulation of ethylene-, jasmonate- and pathogenesis-related genes in tomato fruit (2011)
Journal Article
Tzortzakis, N., Taybi, T., Roberts, R., Singleton, I., Borland, A., & Barnes, J. (2011). Low-level atmospheric ozone exposure induces protection against Botrytis cinerea with down-regulation of ethylene-, jasmonate- and pathogenesis-related genes in tomato fruit. Postharvest Biology and Technology, 61(2-3), 152-159. https://doi.org/10.1016/j.postharvbio.2011.02.013

The aim of this study was to determine if ozone exposure could prevent spoilage in tomato fruit by fungal infection and to explore concomitant changes in expression of genes involved in signal transduction (ethylene, jasmonic acid and C6-aldehydes) a... Read More about Low-level atmospheric ozone exposure induces protection against Botrytis cinerea with down-regulation of ethylene-, jasmonate- and pathogenesis-related genes in tomato fruit.

Effects of ozone exposure on the xerophilic fungus, Eurotium amstelodami IS-SAB-01, isolated from naan bread (2010)
Journal Article
Antony-Babu, S., & Singleton, I. (2011). Effects of ozone exposure on the xerophilic fungus, Eurotium amstelodami IS-SAB-01, isolated from naan bread. International Journal of Food Microbiology, 144(3), 331-336. https://doi.org/10.1016/j.ijfoodmicro.2010.09.019

Xerophilic moulds cause contamination and spoilage of low moisture foods. This study examined the effect of ozone fumigation on growth of a Eurotium species isolated from naan bread. Two ozone treatments were used — a low-level long-term exposure (0.... Read More about Effects of ozone exposure on the xerophilic fungus, Eurotium amstelodami IS-SAB-01, isolated from naan bread.

Quinol-cytochrome c Oxidoreductase and Cytochrome c4 Mediate Electron Transfer during Selenate Respiration in Thauera selenatis (2010)
Journal Article
Lowe, E. C., Bydder, S., Hartshorne, R. S., Tape, H. L. U., Dridge, E. J., Debieux, C. M., Paszkiewicz, K., Singleton, I., Lewis, R. J., Santini, J. M., Richardson, D. J., & Butler, C. S. (2010). Quinol-cytochrome c Oxidoreductase and Cytochrome c4 Mediate Electron Transfer during Selenate Respiration in Thauera selenatis. Journal of Biological Chemistry, 285(24), 18433-18442. https://doi.org/10.1074/jbc.M110.115873

Selenate reductase (SER) from Thauera selenatis is a periplasmic enzyme that has been classified as a type II molybdoenzyme. The enzyme comprises three subunits SerABC, where SerC is an unusual b-heme cytochrome. In the present work the spectropotent... Read More about Quinol-cytochrome c Oxidoreductase and Cytochrome c4 Mediate Electron Transfer during Selenate Respiration in Thauera selenatis.

Effect of ozone on spore germination, spore production and biomass production in two Aspergillus species (2009)
Journal Article
Antony-Babu, S., & Singleton, I. (2009). Effect of ozone on spore germination, spore production and biomass production in two Aspergillus species. Antonie van Leeuwenhoek, 96(4), 413-422. https://doi.org/10.1007/s10482-009-9355-2

The ability of ozone gas to reduce food spoilage is relatively well documented, but the developmental effects of the gas on food spoilage fungi are not well known. In this study two model aspergilli, Aspergillus nidulans and Aspergillus ochraceus wer... Read More about Effect of ozone on spore germination, spore production and biomass production in two Aspergillus species.

Potential use of DNA adducts to detect mutagenic compounds in soil (2008)
Journal Article
Hua, G., Lyons, B., Killham, K., & Singleton, I. (2009). Potential use of DNA adducts to detect mutagenic compounds in soil. Environmental Pollution, 157(3), 916-921. https://doi.org/10.1016/j.envpol.2008.10.026

In this study, three different soils with contrasting features, spiked with 300 mg benzo[a]pyrene (BaP)/kg dry soil, were incubated at 20 °C and 60% water holding capacity for 540 days. At different time points, BaP and {DNA} were extracted and quant... Read More about Potential use of DNA adducts to detect mutagenic compounds in soil.

Biological tools to assess contaminant bioavailability in soils (2008)
Book Chapter
Keatinge, Z., & Singleton, I. (2008). Biological tools to assess contaminant bioavailability in soils. In Chemical Bioavailability in Terrestrial Environment; Developments in Soil Science (479-494). Elsevier. https://doi.org/10.1016/s0166-2481%2807%2932019-9

This chapter provides an overview of the biological tools to assess contaminants bioavailability in soils. Compounds that cause contamination include heavy metals, polyaromatic hydrocarbons (PAHs), pesticides, and chlorinated solvents. For the purpos... Read More about Biological tools to assess contaminant bioavailability in soils.

Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit (2007)
Journal Article
Tzortzakis, N., Singleton, I., & Barnes, J. (2008). Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit. Postharvest Biology and Technology, 47(1), 1-9. https://doi.org/10.1016/j.postharvbio.2007.06.004

Tomato fruit (Lycopersicon esculentum L.) were exposed to ozone concentrations between 0.005 (control) and 5.0 μmol mol−1 up to 13 days at 13 °C, prior to, or following, inoculation by Alternaria alternata or Colletotrichum coccodes (causes of black... Read More about Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit.

Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit (2007)
Journal Article
Tzortzakis, N., Borland, A., Singleton, I., & Barnes, J. (2007). Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit. Postharvest Biology and Technology, 45(3), 317-325. https://doi.org/10.1016/j.postharvbio.2007.03.004

Tomato fruit (Lycopersicon esculentum L. cv. Carousel) were exposed to ozone concentrations ranging between 0.005 (controls) and 1.0 μmol mol−1 at 13 °C and 95% RH. Quality-related attributes and organoleptic characteristics were examined during and... Read More about Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit.

Deployment of low-level ozone-enrichment for the preservation of chilled fresh produce (2007)
Journal Article
Tzortzakis, N., Singleton, I., & Barnes, J. (2007). Deployment of low-level ozone-enrichment for the preservation of chilled fresh produce. Postharvest Biology and Technology, 43(2), 261-270. https://doi.org/10.1016/j.postharvbio.2006.09.005

Tomatoes, strawberries, table grapes and plums were inoculated with Botrytis cinerea (grey mould), transferred to chilled storage (13 °C) and exposed to ‘clean air’ or low-level ozone-enrichment (0.1 μmol mol−1). Ozone-enrichment resulted in a substa... Read More about Deployment of low-level ozone-enrichment for the preservation of chilled fresh produce.

Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-β-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS) (2007)
Journal Article
Hua, G., Broderick, J., Semple, K. T., Killham, K., & Singleton, I. (2007). Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-β-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS). Environmental Pollution, 148(1), 176-181. https://doi.org/10.1016/j.envpol.2006.10.040

Synchronous fluorescence spectroscopy (SFS) was directly applied to rapidly quantify selected polycyclic aromatic hydrocarbons (PAHs: benzo[a]pyrene and pyrene) in aqueous hydroxypropyl-β-cyclodextrin (HPCD) soil extract solutions from a variety of a... Read More about Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-β-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS).

Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting (2006)
Journal Article
Vizard, C. G., Rimmer, D. L., Pless-Mulloli, T., Singleton, I., & Air, V. S. (2006). Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting. Science of the Total Environment, 370(1), 61-69. https://doi.org/10.1016/j.scitotenv.2006.06.006

A study of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/F) concentrations was undertaken in the vicinity of a municipal solid waste incinerator (MSWI) in Newcastle upon Tyne as a result of concerns raised by local re... Read More about Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting.

Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid. (2006)
Journal Article
Lee, M., Kim, M., Singleton, I., Goodfellow, M., & Lee, S. (2006). Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid. Journal of Applied Microbiology, 100(2), 325-333. https://doi.org/10.1111/j.1365-2672.2005.02756.x

Aims:  The aim of the present study was to isolate and characterize a bacterium, strain EN3, capable of using diesel oil as a major carbon and energy source, and to analyse the enhancement of diesel oil degradation by this organism using synthetic my... Read More about Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid..

Environmental Pollution and Restoration: A Role for Bioremediation (2005)
Book Chapter
Philp, J. C., Bamforth, S., Singleton, I., & Atlas, R. M. (2005). Environmental Pollution and Restoration: A Role for Bioremediation. In R. M. Atlas, & J. Philp (Eds.), Bioremediation (1-48). American Society for Microbiology. https://doi.org/10.1128/9781555817596.ch1

With greater understanding of microbial diversity and the development of bioengineering, bioremediation is taking its place as a cost-effective technique in integrated environmental restoration efforts. The major reasons for the control of water and... Read More about Environmental Pollution and Restoration: A Role for Bioremediation.

The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil. (2005)
Journal Article
O’Mahony, M. M., Dobson, A. D., Barnes, J. D., & Singleton, I. (2006). The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil. Chemosphere, 63(2), 307-314. https://doi.org/10.1016/j.chemosphere.2005.07.018

The potential of using ozone for the removal of phenanthrene from several different soils, both alone and in combination with biodegradation using a microbial inoculant (Pseudomonas alcaligenes PA-10), was examined. The greater the water content of t... Read More about The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil..

Metal contamination of urban soils in the vicinity of a municipal waste incinerator: one source among many. (2005)
Journal Article
Rimmer, D. L., Vizard, C. G., Pless-Mulloli, T., Singleton, I., Air, V. S., & Keatinge, Z. A. (2006). Metal contamination of urban soils in the vicinity of a municipal waste incinerator: one source among many. Science of the Total Environment, 356(1-3), 207-216. https://doi.org/10.1016/j.scitotenv.2005.04.037

Concern from local residents about possible contamination with metals and PCDD/F (dioxins and furans) from fugitive and stack emissions from the Byker municipal solid waste incinerator in Newcastle upon Tyne led the City Council to initiate a study o... Read More about Metal contamination of urban soils in the vicinity of a municipal waste incinerator: one source among many..

DDT Resistance and Transformation by Different Microbial Strains Isolated from DDT-Contaminated Soils and Compost Materials (2003)
Journal Article
Kantachote, D., Singleton, I., McClure, N., Naidu, R., Megharaj, M., & Harch, B. D. (2003). DDT Resistance and Transformation by Different Microbial Strains Isolated from DDT-Contaminated Soils and Compost Materials. Compost science & utilization, 11(4), 300-310. https://doi.org/10.1080/1065657x.2003.10702139

Bioremediation is a potential option to treat 1, 1, 1-trichloro-2, 2-bis (4-chlorophenyl) ethane (DDT) contaminated sites. In areas where suitable microbes are not present, the use of DDT resistant microbial inoculants may be necessary. It is vital t... Read More about DDT Resistance and Transformation by Different Microbial Strains Isolated from DDT-Contaminated Soils and Compost Materials.

Changes in microbial properties associated with long-term arsenic and DDT contaminated soils at disused cattle dip sites (2003)
Journal Article
Edvantoro, B., Naidu, R., Megharaj, M., & Singleton, I. (2003). Changes in microbial properties associated with long-term arsenic and DDT contaminated soils at disused cattle dip sites. Ecotoxicology and Environmental Safety, 55(3), 344-351. https://doi.org/10.1016/s0147-6513%2802%2900092-1

This work examined the effect of long-term arsenic (As) and DDT contamination on soil microbial properties at 11 cattle dip sites in northern New South Wales, Australia. Total As in the surface (0–10 cm) soils from these sites ranged from 34 to 2941... Read More about Changes in microbial properties associated with long-term arsenic and DDT contaminated soils at disused cattle dip sites.

Fungal remediation of soils contaminated with persistent organic pollutants (2001)
Book Chapter
Singleton, I. (2001). Fungal remediation of soils contaminated with persistent organic pollutants. In G. M. Gadd (Ed.), Fungi in Bioremediation, (79-96). Cambridge: Cambridge University Press. https://doi.org/10.1017/CBO9780511541780.005

Laboratory-based studies have shown that fungi are able to degrade a wide range of organic pollutants (see other chapters) and have great potential for use as inoculants to remediate contaminated soil. However, soil is a heterogeneous environment and... Read More about Fungal remediation of soils contaminated with persistent organic pollutants.

Resistance of microbial populations in DDT-contaminated and uncontaminated soils (2000)
Journal Article
Kantachote, D., Naidu, R., Singleton, I., McClure, N., & Harch, B. (2001). Resistance of microbial populations in DDT-contaminated and uncontaminated soils. Applied Soil Ecology, 16(1), 85-90. https://doi.org/10.1016/s0929-1393%2800%2900058-5

One DDT-contaminated soil and two uncontaminated soils were used to enumerate DDT-resistant microbes (bacteria, actinomycetes and fungi) by using soil dilution agar plates in media either with 150 μg DDT ml−1 or without DDT at different temperatures... Read More about Resistance of microbial populations in DDT-contaminated and uncontaminated soils.

Pentachlorophenol transformation in soil: a toxicological assessment (2000)
Journal Article
McGrath, R., & Singleton, I. (2000). Pentachlorophenol transformation in soil: a toxicological assessment. Soil Biology and Biochemistry, 32(8-9), 1311-1314. https://doi.org/10.1016/s0038-0717%2800%2900015-8

Bioremediation is recognised as an economically viable method to treat contaminated soil and the use of microbial inoculants, in particular, white-rot fungi, has been proposed to enhance the remediation process. During bioremediation, a variety of po... Read More about Pentachlorophenol transformation in soil: a toxicological assessment.

Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil (2000)
Journal Article
Megharaj, M., Singleton, I., McClure, N. C., & Naidu, R. (2000). Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil. Archives of Environmental Contamination and Toxicology, 38(4), 439-445. https://doi.org/10.1007/s002449910058

Petroleum hydrocarbons are widespread environmental pollutants. Although biodegradation of petroleum hydrocarbons has been the subject of numerous investigations, information on their toxicity to microorganisms in soil is limited, with virtually no w... Read More about Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil.

Effects of long-term contamination of DDT on soil microflora with special reference to soil algae and algal transformation of DDT (2000)
Journal Article
Megharaj, M., Kantachote, D., Singleton, I., & Naidu, R. (2000). Effects of long-term contamination of DDT on soil microflora with special reference to soil algae and algal transformation of DDT. Environmental Pollution, 109(1), 35-42. https://doi.org/10.1016/s0269-7491%2899%2900231-6

DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane) and its principle metabolites, DDE (1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene) and DDD (1,1-dichloro-2,2-bis(p-chlorophenyl)ethane) are widespread environmental contaminants but little informatio... Read More about Effects of long-term contamination of DDT on soil microflora with special reference to soil algae and algal transformation of DDT.

Analysis of ammonia-oxidizing bacteria populations in acid forest soil during conditions of moisture limitation (2000)
Journal Article
Hastings, R. C., Butler, C., Singleton, I., Saunders, J. R., & McCarthy, A. J. (2000). Analysis of ammonia-oxidizing bacteria populations in acid forest soil during conditions of moisture limitation. Letters in applied microbiology, 30(1), 14-18. https://doi.org/10.1046/j.1472-765x.2000.00630.x

Ammonia-oxidizer numbers decreased under conditions of moisture limitation in litter, fermentation and humus layers of forest soil in the field, but the extent of regrowth after rehydration varied between layers. Nitrosospira 16S rRNA genes were ampl... Read More about Analysis of ammonia-oxidizing bacteria populations in acid forest soil during conditions of moisture limitation.

Determination of NTA and EDTA and Speciation of Their Metal Complexes in Aqueous Solution by Capillary Electrophoresis (2000)
Journal Article
Owens, G., Ferguson, V. K., McLaughlin, M. J., Singleton, I., Reid, R. J., & Smith, F. A. (2000). Determination of NTA and EDTA and Speciation of Their Metal Complexes in Aqueous Solution by Capillary Electrophoresis. Environmental science & technology, 34(5), 885-891. https://doi.org/10.1021/es990309m

The interest in the use of chelates for enhancing metal uptake by plants during phytoremediation and their widespread use in plant nutrient research requires that an easy, reproducible method be developed for chelate detection in a variety of systems... Read More about Determination of NTA and EDTA and Speciation of Their Metal Complexes in Aqueous Solution by Capillary Electrophoresis.

Persistence and effects of fenamiphos on native algal populations and enzymatic activities in soil (1999)
Journal Article
Megharaj, M., Singleton, I., Kookana, R., & Naidu, R. (1999). Persistence and effects of fenamiphos on native algal populations and enzymatic activities in soil. Soil Biology and Biochemistry, 31(11), 1549-1553. https://doi.org/10.1016/s0038-0717%2899%2900078-4

Fenamiphos is a commonly and widely used nematicide and insecticide in agriculture but information on its effects on microorganisms in soil is scarce. The microbial role in the persistence of fenamiphos in soil was determined by comparing the relativ... Read More about Persistence and effects of fenamiphos on native algal populations and enzymatic activities in soil.

Diversification of Pseudomonas corrugata 2140 produces new phenotypes altered in GC-FAME, BIOLOG, and in vitro inhibition profiles and taxonomic identification (1999)
Journal Article
Barnett, S. J., Alami, Y., Singleton, I., & Ryder, M. H. (1999). Diversification of Pseudomonas corrugata 2140 produces new phenotypes altered in GC-FAME, BIOLOG, and in vitro inhibition profiles and taxonomic identification. Canadian journal of microbiology, 45(4), 287-298. https://doi.org/10.1139/w99-006

Bacteria are known to rapidly produce new phenotypes, but it is unclear how phenotype "plasticity" relates to studies on the population ecology of bacteria in complex environments. We characterised a collection of 14 spontaneous phenotype variants, d... Read More about Diversification of Pseudomonas corrugata 2140 produces new phenotypes altered in GC-FAME, BIOLOG, and in vitro inhibition profiles and taxonomic identification.

Analysis of Pentachlorophenol in Soils for Use in Bioremediation Studies (1997)
Book Chapter
McGrath, R., & Singleton, I. (1997). Analysis of Pentachlorophenol in Soils for Use in Bioremediation Studies. In Bioremediation Protocols, (169-176). UK: Humana Press. https://doi.org/10.1385/0-89603-437-2%3A169

Pentachlorophenol (PCP) is an envuonmentally persistent chemical that is toxic toward living organisms (1-4) The widespread use of PCP as a general biocide, especially for timber preservation, has led to the contamination of a variety of environments... Read More about Analysis of Pentachlorophenol in Soils for Use in Bioremediation Studies.

Fungal interactions with metals and radionuclides for environmental bioremediation (1996)
Book Chapter
Singleton, I., & Tobin, J. M. (1996). Fungal interactions with metals and radionuclides for environmental bioremediation. In J. C. Frankland, N. Magan, & G. M. Gadd (Eds.), Fungi and environmental change (282-298). Cambridge University Press. https://doi.org/10.1017/CBO9780511753190.018

Microorganisms, including fungi, are known to accumulate metals from their external environment and the possibility of using fungi as a means of treating metal/radionuclide-containing effluents is well recognized (Siegel, Galun & Siegel, 1990; Gadd,... Read More about Fungal interactions with metals and radionuclides for environmental bioremediation.

A method to increase silver biosorption by an industrial strain of Saccharomyces cerevisiae (1996)
Journal Article
Simmons, P., & Singleton, I. (1996). A method to increase silver biosorption by an industrial strain of Saccharomyces cerevisiae. Applied microbiology and biotechnology, 45(1-2), 278-285. https://doi.org/10.1007/s002530050684

Ag+ biosorption by an industrial strain of Saccharomyces cerevisiae was investigated. Older (96 h old) biomass had half the biosorption capacity of younger (24 h old) biomass (0.187 and 0.387 mmol Ag+/g dry mass respectively). Comparisons of cell wal... Read More about A method to increase silver biosorption by an industrial strain of Saccharomyces cerevisiae.

Factors affecting silver biosorption by an industrial strain of Saccharomyces cerevisiae (1996)
Journal Article
Singleton, I., & Simmons, P. (1996). Factors affecting silver biosorption by an industrial strain of Saccharomyces cerevisiae. Journal of Chemical Technology and Biotechnology, 65(1), 21-28. https://doi.org/10.1002/%28SICI%291097-4660%28199601%2965%3A13.0.CO%3B2-E

Factors affecting silver biosorption by Saccharomyces cerevisiae biomass, obtained as a waste product from industry, were examined. Maximum removal of silver from solution was achieved within 5 min. Increasing the concentration of biomass in experime... Read More about Factors affecting silver biosorption by an industrial strain of Saccharomyces cerevisiae.

Metal cation uptake by yeast: a review (1995)
Journal Article
Blackwell, K. J., Singleton, I., & Tobin, J. M. (1995). Metal cation uptake by yeast: a review. Applied microbiology and biotechnology, 43(4), 579-584. https://doi.org/10.1007/s002530050454

This review addresses metal uptake specifically by yeast. Metal uptake may be passive, active or both, depending on the viability of the biomass, and is influenced by a number of environmental and experimental factors. Uptake is typically accompanied... Read More about Metal cation uptake by yeast: a review.

Considerations on the use of commercially available yeast biomass for the treatment of metal-containing effluents (1995)
Journal Article
Simmons, P., Tobin, J. M., & Singleton, I. (1995). Considerations on the use of commercially available yeast biomass for the treatment of metal-containing effluents. Journal of industrial microbiology, 14(3-4), 240-246. doi:10.1007/bf01569934

Three strains ofSaccharomyces cerevisiae and one strain of aCandida sp. obtained from different industrial sources were screened for uptake of silver and copper. Considerable differences in metal uptake capacities were found between the different str... Read More about Considerations on the use of commercially available yeast biomass for the treatment of metal-containing effluents.

Microbial metabolism of xenobiotics: Fundamental and applied research (1994)
Journal Article
Singleton, I. (1994). Microbial metabolism of xenobiotics: Fundamental and applied research. Journal of Chemical Technology and Biotechnology, 59(1), 9-23. doi:10.1002/jctb.280590104

The ability of microorganisms to metabolise xenobiotic compounds has received much attention due to the environmental persistence and toxicity of these chemicals. The microbial degradation of xenobiotics is seen as a cost effective method of removing... Read More about Microbial metabolism of xenobiotics: Fundamental and applied research.