Profiling shifts in protein complement in tomato fruit induced by atmospheric ozone-enrichment and/or wound-inoculation with Botrytis cinerea

Tzortzakis, Nikos; Taybi, Tahar; Antony, Edna; Singleton, Ian; Borland, Anne; Barnes, Jeremy

Abstract

To unravel the mechanism by which low level atmospheric ozone-enrichment (0.05 μmol mol−1) increases the shelf-life of tomato fruit (Lycopersicon esculentum Mill.) by suppressing the growth of pathogens (Botrytis cinerea), protein yield and composition were examined during and following exposure to the gas at 13 °C/95% RH. Ozone-enrichment caused marked changes in protein yield and composition in control tomato fruit and suppressed shifts in the proteome induced by wounding/fungal attack. Wound/fungal-inoculation with B. cinerea resulted in a 7% increase in protein yield, and the down-regulation of at least 32 proteins. A number of proteins affected under ozone and wound/fungal-inoculation treatments are involved in the control of cellular oxidative status. Proteins that may be enhanced under oxidative stress were induced during ozone exposure (e.g. thioredoxin peroxidase-TPX), but suppressed following transfer to ‘clean air’ (e.g. ascorbate peroxidase-APX1). Constitutively-expressed proteins tended to increase reversibly under ozone-treatment, however proteins involved in ripening such as an enzyme related to ethylene biosynthesis (1-aminocyclopropane-1-carboxylate oxidase-ACO) were markedly reduced in ozone-treated tomato fruit but increased in wound-inoculated fruit. Levels of proteins involved in carbohydrate metabolism, pentose phosphate pathway, terpenoid and flavonoid biosynthesis differentiated among the treatments. The presented dataset makes a central contribution to a comprehensive analysis of the manner in which tomato fruit react to ozone-enrichment and/or pathogen infection during storage/transit.

• Journal Article Type: Article
• Acceptance Date: Dec 18, 2012
• Online Publication Date: Jan 15, 2013
• Publication Date: 2013-04
• Deposit Date: Aug 2, 2016
• Journal: Postharvest Biology and Technology
• Print ISSN: 0925-5214
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 78
• Pages: 67-75
• DOI: https://doi.org/10.1016/j.postharvbio.2012.12.005
• Keywords: Tomato fruit, ozone, botrytis cinerea, modified atmosphere storage, proteomics,
• Public URL: http://researchrepository.napier.ac.uk/Output/322440
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0925521412002712