All Outputs (40)

The STR/ort mouse model of spontaneous osteoarthritis – an update (2016)
Journal Article
Staines, K. A., Poulet, B., Wentworth, D. N., & Pitsillides, A. A. (2017). The STR/ort mouse model of spontaneous osteoarthritis – an update. Osteoarthritis and Cartilage, 25(6), 802-808. https://doi.org/10.1016/j.joca.2016.12.014

Osteoarthritis is a degenerative joint disease and a world-wide healthcare burden. Characterized by cartilage degradation, subchondral bone thickening and osteophyte formation, osteoarthritis inflicts much pain and suffering, for which there are curr... Read More about The STR/ort mouse model of spontaneous osteoarthritis – an update.

The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures (2016)
Journal Article
Houston, D. A., Myers, K., MacRae, V. E., Staines, K. A., & Farquharson, C. (2016). The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures. Calcified Tissue International, 99(5), 510-524. https://doi.org/10.1007/s00223-016-0176-9

Sustained exposure to high levels of parathyroid hormone (PTH), as observed in hyperparathyroidism, are catabolic to bone. The increase in the RANKL/OPG ratio in response to continuous PTH, resulting in increased osteoclastogenesis is well establishe... Read More about The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures.

Models of ex vivo explant cultures: applications in bone research (2016)
Journal Article
Marino, S., Staines, K. A., Brown, G., Howard-Jones, R. A., & Adamczyk, M. (2016). Models of ex vivo explant cultures: applications in bone research. BoneKEy Reports, 5, 818. https://doi.org/10.1038/bonekey.2016.49

Ex vivo explant culture models are powerful tools in bone research. They allow investigation of bone and cartilage responses to specific stimuli in a controlled manner that closely mimics the in vivo processes. Because of limitations in obtaining he... Read More about Models of ex vivo explant cultures: applications in bone research.

Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model (2016)
Journal Article
Staines, K. A., Madi, K., Mirczuk, S. M., Parker, S., Burleigh, A., Poulet, B., …Pitsillides, A. A. (2016). Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model. Arthritis a

OBJECTIVE: To explore whether aberrant transient chondrocyte behaviors occur in the joints of STR/Ort mice (which spontaneously develop osteoarthritis [OA]) and whether they are attributable to an endochondral growth defect. METHODS: Knee joints f... Read More about Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model.

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification (2016)
Journal Article
Houston, D. A., Staines, K. A., MacRae, V. E., & Farquharson, C. (2016). Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification. Journal of Visualized Experiments, 118(118), Article e54978. https://doi.org/10.3791/5497

The fundamental process of endochondral ossification is under tight regulation in the healthy individual so as to prevent disturbed development and/or longitudinal bone growth. As such, it is imperative that we further our understanding of the underp... Read More about Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification.

New developments in osteoarthritis and cartilage biology (2016)
Journal Article
Poulet, B., & Staines, K. A. (2016). New developments in osteoarthritis and cartilage biology. Current Opinion in Pharmacology, 28, 8-13. https://doi.org/10.1016/j.coph.2016.02.009

Osteoarthritis (OA) is a degenerative joint disease and the most common form of arthritis. Characterised by articular cartilage loss, subchondral bone thickening and osteophyte formation, the OA joint afflicts much pain and disability. Whilst OA has... Read More about New developments in osteoarthritis and cartilage biology.

E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models (2015)
Journal Article
Staines, K. A., Prideaux, M., Allen, S., Buttle, D. J., Pitsillides, A. A., & Farquharson, C. (2016). E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models. Journal of Cellul

The transmembrane glycoprotein E11 is considered critical in early osteoblast–osteocyte transitions (osteocytogenesis), however its function and regulatory mechanisms are still unknown. Using the late osteoblast MLO-A5 cell line we reveal increased E... Read More about E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models.

Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing (2015)
Journal Article
Zaman, G., Farquharson, C., Staines, K. A., Newton, P. T., Dudhia, J., Chenu, C., …Dhoot, G. K. (2016). Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing. Histochemistry and Cell Biology, 145(1), 67-79. https://doi.org/10.1007/s00418-015-1365-8

SULF1/SULF2 enzymes regulate cell signalling that impacts the growth and differentiation of many tissues. To determine their possible role in cartilage and bone growth or repair, their expression was examined during development and bone fracture heal... Read More about Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing.

Phospho1 deficiency transiently modifies bone architecture yet produces consistent modification in osteocyte differentiation and vascular porosity with ageing (2015)
Journal Article
Javaheri, B., Carriero, A., Staines, K. A., Chang, Y., Houston, D. A., Oldknow, K. J., …Pitsillides, A. A. (2015). Phospho1 deficiency transiently modifies bone architecture yet produces consistent modification in osteocyte differentiation and vascular porosity with ageing. Bone, 81, 277-291. https://doi.org/10.1016/j.bone.2015.07.035

PHOSPHO1 is one of principal proteins involved in initiating bone matrix mineralisation. Recent studies have found that Phospho1 KO mice (Phospho1-R74X) display multiple skeletal abnormalities with spontaneous fractures, bowed long bones, osteomalaci... Read More about Phospho1 deficiency transiently modifies bone architecture yet produces consistent modification in osteocyte differentiation and vascular porosity with ageing.

Effects of etidronate on the Enpp1−/− mouse model of generalized arterial calcification of infancy (2015)
Journal Article
Huesa, C., Staines, K. A., Millán, J. L., & MacRae, V. E. (2015). Effects of etidronate on the Enpp1−/− mouse model of generalized arterial calcification of infancy. International Journal of Molecular Medicine, 36(1), 159-165. https://doi.org/10.3892

Generalized arterial calcification of infancy (GACI) is an autosomal recessive disorder of spontaneous infantile arterial and periarticular calcification which is attributed to mutations in the ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp... Read More about Effects of etidronate on the Enpp1−/− mouse model of generalized arterial calcification of infancy.

MMP and TIMP temporal gene expression during osteocytogenesis (2015)
Journal Article
Prideaux, M., Staines, K. A., Jones, E. R., Riley, G. P., Pitsillides, A. A., & Farquharson, C. (2015). MMP and TIMP temporal gene expression during osteocytogenesis. Gene Expression Patterns, 18(1-2), 29-36. https://doi.org/10.1016/j.gep.2015.04.004

Osteocytes within bone differentiate from osteoblast precursors which reside in a mineralised extracellular matrix (ECM). Fully differentiated osteocytes are critical for bone development and function but the factors that regulate this differentiatio... Read More about MMP and TIMP temporal gene expression during osteocytogenesis.

Increased linear bone growth by GH in the absence of SOCS2 is independent of IGF-1: SOCS2 REGULATION OF GH INDUCED GROWTH (2015)
Journal Article
Dobie, R., Ahmed, S. F., Staines, K. A., Pass, C., Jasim, S., MacRae, V. E., & Farquharson, C. (2015). Increased linear bone growth by GH in the absence of SOCS2 is independent of IGF-1: SOCS2 REGULATION OF GH INDUCED GROWTH. Journal of Cellular Physiolog

Growth hormone (GH) signaling is essential for postnatal linear bone growth, but the relative importance of GHs actions on the liver and/or growth plate cartilage remains unclear. The importance of liver derived insulin like-growth factor-1 (IGF-1) f... Read More about Increased linear bone growth by GH in the absence of SOCS2 is independent of IGF-1: SOCS2 REGULATION OF GH INDUCED GROWTH.

miRNA-221 and miRNA-222 synergistically function to promote vascular calcification: MIR221/222 PROMOTE VASCULAR CALCIFICATION (2013)
Journal Article
Mackenzie, N. C. W., Staines, K. A., Zhu, D., Genever, P., & MacRae, V. E. (2014). miRNA-221 and miRNA-222 synergistically function to promote vascular calcification: MIR221/222 PROMOTE VASCULAR CALCIFICATION. Cell Biochemistry and Function, 32(2), 209-21

Vascular calcification shares many similarities with skeletal mineralisation and involves the phenotypic trans-differentiation of vascular smooth muscle cells (VSMCs) to osteoblastic cells within a calcified environment. Various microRNAs (miRs) are... Read More about miRNA-221 and miRNA-222 synergistically function to promote vascular calcification: MIR221/222 PROMOTE VASCULAR CALCIFICATION.

Cartilage to bone transitions in health and disease (2013)
Journal Article
Staines, K. A., Pollard, A. S., McGonnell, I. M., Farquharson, C., & Pitsillides, A. A. (2013). Cartilage to bone transitions in health and disease. Journal of Endocrinology, 219(1), R1-R12. https://doi.org/10.1530/joe-13-0276

Aberrant redeployment of the 'transient' events responsible for bone development and postnatal longitudinal growth has been reported in some diseases in what is otherwise inherently 'stable' cartilage. Lessons may be learnt from the molecular mechani... Read More about Cartilage to bone transitions in health and disease.

Identification of novel regulators of osteoblast matrix mineralization by time series transcriptional profiling (2013)
Journal Article
Staines, K. A., Zhu, D., Farquharson, C., & MacRae, V. E. (2014). Identification of novel regulators of osteoblast matrix mineralization by time series transcriptional profiling. Journal of Bone and Mineral Metabolism, 32(3), 240-251. https://doi.org/10.1007/s00774-013-0493-2

Bone mineralization is a carefully orchestrated process, regulated by a number of promoters and inhibitors that function to ensure effective hydroxyapatite formation. Here we sought to identify new regulators of this process through a time series mic... Read More about Identification of novel regulators of osteoblast matrix mineralization by time series transcriptional profiling.

Chondrogenic ATDC5 cells: An optimised model for rapid and physiological matrix mineralisation (2012)
Journal Article
Newton, P. T., Staines, K. .. . A., Spevak, L., Boskey, A. L., Teixeira, C. C., Macrae, V. E., …Farquharson, C. (2012). Chondrogenic ATDC5 cells: An optimised model for rapid and physiological matrix mineralisation. International Journal of Molecular Me

The development of chondrogenic cell lines has led to major advances in the understanding of how chondrocyte differentiation is regulated, and has uncovered many signalling pathways and gene regulatory mechanisms required to maintain normal function.... Read More about Chondrogenic ATDC5 cells: An optimised model for rapid and physiological matrix mineralisation.

MEPE is a novel regulator of growth plate cartilage mineralization (2012)
Journal Article
Staines, K. A., Mackenzie, N. C. W., Clarkin, C. E., Zelenchuk, L., Rowe, P. S., MacRae, V. E., & Farquharson, C. (2012). MEPE is a novel regulator of growth plate cartilage mineralization. Bone, 51(3), 418-430. https://doi.org/10.1016/j.bone.2012.06.022

Matrix extracellular phosphoglycoprotein (MEPE) belongs to the SIBLING protein family which play key roles in biomineralization. Although the growth plates of MEPE-overexpressing mice display severe morphological disruption, the expression and functi... Read More about MEPE is a novel regulator of growth plate cartilage mineralization.

Cartilage development and degeneration: a Wnt Wnt situation - cartilage development and degeneration: a WNT WNT situation (2012)
Journal Article
Staines, K. A., MacRae, V. E., & Farquharson, C. (2012). Cartilage development and degeneration: a Wnt Wnt situation - cartilage development and degeneration: a WNT WNT situation. Cell Biochemistry and Function, 30(8), 633-642. https://doi.org/10.1002/cbf

The Wnt signaling pathway plays a crucial role in the development and homeostasis of a variety of adult tissues and, as such, is emerging as an important therapeutic target for numerous diseases. Factors involved in the Wnt pathway are expressed thro... Read More about Cartilage development and degeneration: a Wnt Wnt situation - cartilage development and degeneration: a WNT WNT situation.

The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling (2012)
Journal Article
Staines, K. A., MacRae, V. E., & Farquharson, C. (2012). The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling. Journal of Endocrinology, 214(3), 241-255. https://doi.org/10.1530/joe-12-0143

The small integrin-binding ligand N-linked glycoprotein (SIBLING) family consists of osteopontin, bone sialoprotein, dentin matrix protein 1, dentin sialophosphoprotein and matrix extracellular phosphoglycoprotein. These proteins share many structura... Read More about The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling.

The skeleton: no bones about it (2011)
Journal Article
Farquharson, C., & Staines, K. (2011). The skeleton: no bones about it. Journal of Endocrinology, 211(2), 107-108. doi:10.1530/joe-11-0274

NO abstract available.