Outputs (215)

Overexpression of TIMP-3 in Chondrocytes Produces Transient Reduction in Growth Plate Length but Permanently Reduces Adult Bone Quality and Quantity (2016)
Journal Article
Poulet, B., Liu, K., Plumb, D., Vo, P., Shah, M., Staines, K., Sampson, A., Nakamura, H., Nagase, H., Carriero, A., Shefelbine, S., Pitsillides, A. A., & Bou-Gharios, G. (2016). Overexpression of TIMP-3 in Chondrocytes Produces Transient Reduction in Growth Plate Length but Permanently Reduces Adult Bone Quality and Quantity. PLOS ONE, 11(12), Article e0167971. https://doi.org/10.1371/journal.pone.0167971

Bone development and length relies on the growth plate formation, which is dependent on degradative enzymes such as MMPs. Indeed, deletion of specific members of this enzyme family in mice results in important joint and bone abnormalities, suggesting... Read More about Overexpression of TIMP-3 in Chondrocytes Produces Transient Reduction in Growth Plate Length but Permanently Reduces Adult Bone Quality and Quantity.

Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model (2016)
Journal Article
Staines, K. A., Madi, K., Mirczuk, S. M., Parker, S., Burleigh, A., Poulet, B., Hopkinson, M., Bodey, A. J., Fowkes, R. C., Farquharson, C., Lee, P. D., & Pitsillides, A. A. (2016). Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model. Arthritis and Rheumatology, 68(4), 880-891. https://doi.org/10.1002/art.39508

OBJECTIVE: To explore whether aberrant transient chondrocyte behaviors occur in the joints of STR/Ort mice (which spontaneously develop osteoarthritis [OA]) and whether they are attributable to an endochondral growth defect. METHODS: Knee joints f... Read More about Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model.

Immunological homeostasis at the ovine placenta may reflect the degree of maternal foetal interaction (2019)
Journal Article
Wattegedera, S. R., Doull, L. E., Goncheva, M. I., Wheelhouse, N., Watson, D. M., Pearce, J., Benavides, J., Palarea-Albaladejo, J., Mcinnes, C. J., Ballingall, K., & Entrican, G. (2019). Immunological homeostasis at the ovine placenta may reflect the degree of maternal foetal interaction. Frontiers in Immunology, 9, https://doi.org/10.3389/fimmu.2018.03025

Successful mammalian pregnancies are a result of complex physiological, endocrinological and immunological processes that combine to create an environment where the mother is tolerant to the semi-allogeneic fetus. Our knowledge of the mechanisms that... Read More about Immunological homeostasis at the ovine placenta may reflect the degree of maternal foetal interaction.

Effects of etidronate on the Enpp1−/− mouse model of generalized arterial calcification of infancy (2015)
Journal Article
Huesa, C., Staines, K. A., Millán, J. L., & MacRae, V. E. (2015). Effects of etidronate on the Enpp1−/− mouse model of generalized arterial calcification of infancy. International Journal of Molecular Medicine, 36(1), 159-165. https://doi.org/10.3892/ijmm.2015.2212

Generalized arterial calcification of infancy (GACI) is an autosomal recessive disorder of spontaneous infantile arterial and periarticular calcification which is attributed to mutations in the ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp... Read More about Effects of etidronate on the Enpp1−/− mouse model of generalized arterial calcification of infancy.

Increased linear bone growth by GH in the absence of SOCS2 is independent of IGF-1: SOCS2 REGULATION OF GH INDUCED GROWTH (2015)
Journal Article
Dobie, R., Ahmed, S. F., Staines, K. A., Pass, C., Jasim, S., MacRae, V. E., & Farquharson, C. (2015). Increased linear bone growth by GH in the absence of SOCS2 is independent of IGF-1: SOCS2 REGULATION OF GH INDUCED GROWTH. Journal of Cellular Physiology, 230(11), 2796-2806. https://doi.org/10.1002/jcp.25006

Growth hormone (GH) signaling is essential for postnatal linear bone growth, but the relative importance of GHs actions on the liver and/or growth plate cartilage remains unclear. The importance of liver derived insulin like-growth factor-1 (IGF-1) f... Read More about Increased linear bone growth by GH in the absence of SOCS2 is independent of IGF-1: SOCS2 REGULATION OF GH INDUCED GROWTH.

miRNA-221 and miRNA-222 synergistically function to promote vascular calcification: MIR221/222 PROMOTE VASCULAR CALCIFICATION (2013)
Journal Article
Mackenzie, N. C. W., Staines, K. A., Zhu, D., Genever, P., & MacRae, V. E. (2014). miRNA-221 and miRNA-222 synergistically function to promote vascular calcification: MIR221/222 PROMOTE VASCULAR CALCIFICATION. Cell Biochemistry and Function, 32(2), 209-216. https://doi.org/10.1002/cbf.3005

Vascular calcification shares many similarities with skeletal mineralisation and involves the phenotypic trans-differentiation of vascular smooth muscle cells (VSMCs) to osteoblastic cells within a calcified environment. Various microRNAs (miRs) are... Read More about miRNA-221 and miRNA-222 synergistically function to promote vascular calcification: MIR221/222 PROMOTE VASCULAR CALCIFICATION.

Cartilage to bone transitions in health and disease (2013)
Journal Article
Staines, K. A., Pollard, A. S., McGonnell, I. M., Farquharson, C., & Pitsillides, A. A. (2013). Cartilage to bone transitions in health and disease. Journal of Endocrinology, 219(1), R1-R12. https://doi.org/10.1530/joe-13-0276

Aberrant redeployment of the 'transient' events responsible for bone development and postnatal longitudinal growth has been reported in some diseases in what is otherwise inherently 'stable' cartilage. Lessons may be learnt from the molecular mechani... Read More about Cartilage to bone transitions in health and disease.

Chondrogenic ATDC5 cells: An optimised model for rapid and physiological matrix mineralisation (2012)
Journal Article
Newton, P. T., Staines, K. .. . A., Spevak, L., Boskey, A. L., Teixeira, C. C., Macrae, V. E., Canfield, A. E., & Farquharson, C. (2012). Chondrogenic ATDC5 cells: An optimised model for rapid and physiological matrix mineralisation. International Journal of Molecular Medicine, 30(5), 1187-1193. https://doi.org/10.3892/ijmm.2012.1114

The development of chondrogenic cell lines has led to major advances in the understanding of how chondrocyte differentiation is regulated, and has uncovered many signalling pathways and gene regulatory mechanisms required to maintain normal function.... Read More about Chondrogenic ATDC5 cells: An optimised model for rapid and physiological matrix mineralisation.

The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling (2012)
Journal Article
Staines, K. A., MacRae, V. E., & Farquharson, C. (2012). The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling. Journal of Endocrinology, 214(3), 241-255. https://doi.org/10.1530/joe-12-0143

The small integrin-binding ligand N-linked glycoprotein (SIBLING) family consists of osteopontin, bone sialoprotein, dentin matrix protein 1, dentin sialophosphoprotein and matrix extracellular phosphoglycoprotein. These proteins share many structura... Read More about The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling.

Cartilage development and degeneration: a Wnt Wnt situation - cartilage development and degeneration: a WNT WNT situation (2012)
Journal Article
Staines, K. A., MacRae, V. E., & Farquharson, C. (2012). Cartilage development and degeneration: a Wnt Wnt situation - cartilage development and degeneration: a WNT WNT situation. Cell Biochemistry and Function, 30(8), 633-642. https://doi.org/10.1002/cbf.2852

The Wnt signaling pathway plays a crucial role in the development and homeostasis of a variety of adult tissues and, as such, is emerging as an important therapeutic target for numerous diseases. Factors involved in the Wnt pathway are expressed thro... Read More about Cartilage development and degeneration: a Wnt Wnt situation - cartilage development and degeneration: a WNT WNT situation.

The skeleton: no bones about it (2011)
Journal Article
Farquharson, C., & Staines, K. (2011). The skeleton: no bones about it. Journal of Endocrinology, 211(2), 107-108. doi:10.1530/joe-11-0274

NO abstract available.

Ferlavirus-related deaths in a collection of viperid snakes (2018)
Journal Article
Flach, E. J., Dagleish, M. P., Feltrer, Y., Gill, I. S., Marschang, R. E., Masters, N., Orós, J., Pocknell, A., Rendle, M. E., Strike, T., Tapley, B., & Wheelhouse, N. M. (2018). Ferlavirus-related deaths in a collection of viperid snakes. Journal of Zoo and Wildlife Medicine, 49(4), 983-995. https://doi.org/10.1638/2017-0223.1

Between June and October 2013 26 snakes of six viperid species kept in two adjoining rooms died (n = 16) or were euthanized on medical (1) or welfare grounds (9). Two were from the main zoo collection, but the other 24 had been imported and quarantin... Read More about Ferlavirus-related deaths in a collection of viperid snakes.

Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease (2018)
Journal Article
Hooper, K. M., Barlow, P. G., Henderson, P., & Stevens, C. (2019). Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease. Inflammatory Bowel Diseases, 25(4), 661-671. https://doi.org/10.1093/ibd/izy380

Inflammatory Bowel Disease (IBD), which includes Crohn's disease (CD) and ulcerative colitis, is characterised by chronic inflammation of the gastrointestinal tract. Aetiology involves a combination of genetic and environmental factors resulting in a... Read More about Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease.

Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain (2017)
Journal Article
Velasova, M., Damaso, A., Prakashbabu, B. C., Gibbons, J., Wheelhouse, N., Longbottom, D., Van Winden, S., Green, M., & Guitian, J. (2017). Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain. Journal of Dairy Science, 100(11), 9215-9233. https://doi.org/10.3168/jds.2016-11863

To implement appropriate and effective disease control programs at national level, up-to-date and unbiased information on disease frequency is needed. The aim of this study was to estimate the prevalence of selected endemic infectious diseases in th... Read More about Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain.

Preferential activation of HIF-2 adaptive mechanisms in neuronal-like cells in response to hypoxia (2017)
Presentation / Conference Contribution
Fraser, J., Baudel, M., Rae, M., Darlison, M., & Poole, A. (2017, April). Preferential activation of HIF-2 adaptive mechanisms in neuronal-like cells in response to hypoxia. Poster presented at British Neuroscience Association - festival of neuroscience 2017

Stroke is a leading cause of death and disability worldwide. Blockage, or occlusion, of cerebral arteries causes irreversible neuronal damage as disrupted blood flow starves neurones of oxygen and glucose. The hypoxia inducible factors (HIFs) are mas... Read More about Preferential activation of HIF-2 adaptive mechanisms in neuronal-like cells in response to hypoxia.

Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation (2017)
Journal Article
Buttle, D. J., Pitsillides, A. A., Staines, K. A., Javaheri, B., Hohenstein, P., Fleming, R., Ikpegbu, E., Unger, E., Hopkinson, M., Buttle, D., Pitsillides, A., & Farquharson, C. (2017). Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation. Journal of Cellular Physiology, 232(11), 3006-3019. https://doi.org/10.1002/jcp.25999

The transmembrane glycoprotein E11/Podoplanin (Pdpn) has been implicated in the initial stages of osteocyte differentiation. However, its precise function and regulatory mechanisms are still unknown. Due to the known embryonic lethality induced by gl... Read More about Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation.

Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS (2017)
Journal Article
Livingstone, M., Wheelhouse, N., Ensor, H., Rocchi, M., Maley, S., Aitchison, K., Wattegedera, S., Wilson, K., Sait, M., Siarkou, V., Vretou, E., Entrican, G., Dagleish, M., & Longbottom, D. (2017). Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS. PLOS ONE, 12(5), Article e0177653. https://doi.org/10.1371/journal.pone.0177653

This study investigated the pathogenesis of two variant strains (LLG and POS) of Chlamydia abortus, in comparison to a typical wild-type strain (S26/3) which is known to be responsible for late term abortion in small ruminants. Challenge with the thr... Read More about Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS.

Estimating excess length of stay due to healthcare-associated infections: A systematic review and meta-analysis of statistical methodology (2018)
Journal Article
Manoukian, S., Stewart, S., Dancer, S., Graves, N., Mason, H., McFarland, A., Robertson, C., & Reilly, J. (2018). Estimating excess length of stay due to healthcare-associated infections: A systematic review and meta-analysis of statistical methodology. Journal of Hospital Infection, https://doi.org/10.1016/j.jhin.2018.06.003

Background Healthcare-associated infection (HAI) affects millions of patients worldwide. HAI is associated with increased healthcare costs, owing primarily to increased hospital length of stay (LOS) but calculating these costs is complicated due to... Read More about Estimating excess length of stay due to healthcare-associated infections: A systematic review and meta-analysis of statistical methodology.

Diurnal Variation in Physiological and Immune Responses to Endurance Sport in Highly Trained Runners in a Hot and Humid Environment (2018)
Journal Article
Boukelia, Z., Gomes, E. C., & Florida-James, G. D. (2018). Diurnal Variation in Physiological and Immune Responses to Endurance Sport in Highly Trained Runners in a Hot and Humid Environment. Oxidative Medicine and Cellular Longevity, 2018, Article 3402143. https://doi.org/10.1155/2018/3402143

Purpose. The purpose of this study was to investigate the physiological and immunological response of highly trained runners to an intense bout of exercise performed at two different times of day, in a hot, humid environment. Methods. Using a crossov... Read More about Diurnal Variation in Physiological and Immune Responses to Endurance Sport in Highly Trained Runners in a Hot and Humid Environment.

Quantifying the relative effect of environmental contamination on surgical ward MRSA incidence: An exploratory analysis (2018)
Journal Article
Lee, X. J., Pettitt, A. N., & Dancer, S. J. (2018). Quantifying the relative effect of environmental contamination on surgical ward MRSA incidence: An exploratory analysis. Infection, Disease & Health, https://doi.org/10.1016/j.idh.2018.02.005

Background: To investigate and quantify the contribution of environmental contamination towards methicillin-resistant Staphylococcus aureus (MRSA) incidence observed in a hospital ward using stochastic modelling. Methods: A non-homogeneous Poisson pr... Read More about Quantifying the relative effect of environmental contamination on surgical ward MRSA incidence: An exploratory analysis.