Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation

Buttle, David J.; Pitsillides, Andrew A.; Staines, Katherine A.; Javaheri, Behzad; Hohenstein, Peter; Fleming, Robert; Ikpegbu, Ekele; Unger, Erin; Hopkinson, Mark; Buttle, David; Pitsillides, Andrew; Farquharson, Colin

Abstract

The transmembrane glycoprotein E11/Podoplanin (Pdpn) has been implicated in the initial stages of osteocyte differentiation. However, its precise function and regulatory mechanisms are still unknown. Due to the known embryonic lethality induced by global Pdpn deletion, we have herein explored the effect of bone specific Pdpn knockdown on osteocyte form and function in the post-natal mouse. Extensive skeletal phenotyping of male and female 6-week-old Oc-cre;Pdpnflox/flox (cKO) mice and their Pdpnflox/flox controls (fl/fl) has revealed that Pdpn deletion significantly compromises tibial cortical bone microarchitecture in both sexes, albeit to different extents (P

Citation

Buttle, D. J., Pitsillides, A. A., Staines, K. A., Javaheri, B., Hohenstein, P., Fleming, R., Ikpegbu, E., Unger, E., Hopkinson, M., Buttle, D., Pitsillides, A., & Farquharson, C. (2017). Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation. Journal of Cellular Physiology, 232(11), 3006-3019. https://doi.org/10.1002/jcp.25999

• Journal Article Type: Article
• Acceptance Date: May 8, 2017
• Online Publication Date: May 10, 2017
• Publication Date: 2017-11
• Deposit Date: May 8, 2017
• Publicly Available Date: Nov 2, 2017
• Journal: Journal of Cellular Physiology
• Print ISSN: 0021-9541
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 232
• Issue: 11
• Pages: 3006-3019
• DOI: https://doi.org/10.1002/jcp.25999
• Keywords: Osteocytes; E11; osteocalcin; osteoblasts; dendrite
• Public URL: http://researchrepository.napier.ac.uk/Output/841066
• Contract Date: Nov 2, 2017