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Gordonia defluvii sp. nov., an actinomycete isolated from activated sludge foam.

Soddell, Jacques A; Stainsby, Fiona M; Eales, Kathryn L; Seviour, Robert J; Goodfellow, Michael

Authors

Jacques A Soddell

Kathryn L Eales

Robert J Seviour

Michael Goodfellow



Abstract

Three strains of non-motile, Gram-positive, filamentous actinomycetes, isolates J4T, J5 and J59, initially recognized microscopically in activated sludge foam by their distinctive branching patterns, were isolated by micromanipulation. The taxonomic positions of the isolates were determined using a polyphasic approach. Almost-complete 16S rRNA gene sequences of the isolates were aligned with corresponding sequences of representatives of the suborder Corynebacterineae and phylogenetic trees were inferred using three tree-making algorithms. The organisms formed a distinct phyletic line in the Gordonia 16S rRNA gene tree. The three isolates showed 16S rRNA gene sequence similarities within the range 96.9–97.2 % with their nearest phylogenetic neighbours, namely Gordonia bronchialis DSM 43247T and Gordonia terrae DSM 43249T. Strain J4T was shown to have a chemotaxonomic profile typical of the genus Gordonia and was readily distinguished from representatives of the genus on the basis of Curie-point pyrolysis mass spectrometric data. The isolates shared nearly identical phenotypic profiles that distinguished them from representatives of the most closely related Gordonia species. It is evident from the genotypic and phenotypic data that the three isolates belong to a novel Gordonia species. The name proposed for this taxon is Gordonia defluvii sp. nov.; the type strain is J4T (=DSM 44981T=NCIMB 14149T).

Citation

Soddell, J. A., Stainsby, F. M., Eales, K. L., Seviour, R. J., & Goodfellow, M. (2006). Gordonia defluvii sp. nov., an actinomycete isolated from activated sludge foam. International Journal of Systematic and Evolutionary Microbiology, 56, 2265-2269. https://doi.org/10.1099/ijs.0.64034-0

Journal Article Type Article
Publication Date Oct 1, 2006
Deposit Date Jul 18, 2008
Print ISSN 1466-5026
Electronic ISSN 1466-5034
Publisher Microbiology Society
Peer Reviewed Peer Reviewed
Volume 56
Pages 2265-2269
DOI https://doi.org/10.1099/ijs.0.64034-0
Keywords Foam; Activated sludge; Bacteria; Actinomycetes; Taxonomy; Phylogenetic trees; Algorithms; Curie-point pyrolysis mass spectrometry; Gordonia defluvii sp. nov. ;
Public URL http://researchrepository.napier.ac.uk/id/eprint/1731
Publisher URL http://dx.doi.org/10.1099/ijs.0.64034-0






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