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Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAAreceptor (1992)
Journal Article
Zaman, S. H., Harvey, R. J., Barnard, E. A., & Darlison, M. G. (1992). Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAAreceptor. FEBS Letters, 307(3), 351-354. https://doi.org/10.1016/0014-5793%2892%2980711-o

We have previously reported [(1991) EMBO J. 10, 3239–3245] the sequence of an invertebrate γ‐aminobutyric acid (GABA) type A (GABAA) receptor polypeptide which forms homo‐oligomeric GABA‐gated, bicuculline‐sensitive, chloride‐ion channels upon hetero... Read More about Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAAreceptor.

Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil (2000)
Journal Article
Megharaj, M., Singleton, I., McClure, N. C., & Naidu, R. (2000). Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil. Archives of Environmental Contamination and Toxicology, 38(4), 439-445. https://doi.org/10.1007/s002449910058

Petroleum hydrocarbons are widespread environmental pollutants. Although biodegradation of petroleum hydrocarbons has been the subject of numerous investigations, information on their toxicity to microorganisms in soil is limited, with virtually no w... Read More about Influence of Petroleum Hydrocarbon Contamination on Microalgae and Microbial Activities in a Long-Term Contaminated Soil.

Structural and functional basis for GABAA receptor heterogeneity (1988)
Journal Article
Levitan, E. S., Schofield, P. R., Burt, D. R., Rhee, L. M., Wisden, W., Köhler, M., …Seeburg, P. H. (1988). Structural and functional basis for GABAA receptor heterogeneity. Nature, 335(6185), 76-79. https://doi.org/10.1038/335076a0

When γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in vertebrate brain, binds to its receptor it activates a chloride channel. Neurotransmitter action at the GABAA receptor is potentiated by both benzodiazepines and barbiturates w... Read More about Structural and functional basis for GABAA receptor heterogeneity.

Distinct GABAA receptor α subunit mRNAs show differential patterns of expression in bovine brain (1988)
Journal Article
Wisden, W., Morris, B. J., Darlison, M. G., Hunt, S. P., & Barnard, E. A. (1988). Distinct GABAA receptor α subunit mRNAs show differential patterns of expression in bovine brain. Neuron, 1(10), 937-947. https://doi.org/10.1016/0896-6273%2888%2990151-1

Specific oligonucleotide probes have been used to visualize the regional and cellular distribution of the mRNAs encoding three structurally distinct GABA A receptor α subunits in bovine brain. In situ hybridization analysis showed that these transcri... Read More about Distinct GABAA receptor α subunit mRNAs show differential patterns of expression in bovine brain.

Cloning of a cDNA that encodes an invertebrate glutamate receptor subunit (1991)
Journal Article
Hutton, M. L., Harvey, R. J., Barnard, E. A., & Darlison, M. (1991). Cloning of a cDNA that encodes an invertebrate glutamate receptor subunit. FEBS Letters, 292(1-2), 111-114. https://doi.org/10.1016/0014-5793%2891%2980846-u

A full‐length cDNA which encodes a putative glutamate receptor polypeptide was isolated from the pond snailLymnaea stagnalis, using a short stretch of exonic sequence and two variants of the polymerase chain reaction. In this first comparison of inve... Read More about Cloning of a cDNA that encodes an invertebrate glutamate receptor subunit.

GABAA receptor subunit genes as candidate genes for bipolar affective disorder—an association analysis (1992)
Journal Article
Walsh, C., Hicks, A. A., Sham, P., Castle, D., Hunt, N., Clements, A., Sander, T., Murray, R., Darlison, M. G., & Gill, M. (1992). GABAA receptor subunit genes as candidate genes for bipolar affective disorder—an association analysis. Psychiatric Genetics, 2(4), 239-248. https://doi.org/10.1097/00041444-199210000-00002

Polymorphic DNA segments can be used as genetic markers to test for allelic association between a disease and a candidate gene locus. Here we report the results of a study of 60 unrelated bipolar patients in which we sought for evidence of associatio... Read More about GABAA receptor subunit genes as candidate genes for bipolar affective disorder—an association analysis.

Biological tools to assess contaminant bioavailability in soils (2008)
Book Chapter
Keatinge, Z., & Singleton, I. (2008). Biological tools to assess contaminant bioavailability in soils. In Chemical Bioavailability in Terrestrial Environment; Developments in Soil Science (479-494). Elsevier. https://doi.org/10.1016/s0166-2481%2807%2932019-9

This chapter provides an overview of the biological tools to assess contaminants bioavailability in soils. Compounds that cause contamination include heavy metals, polyaromatic hydrocarbons (PAHs), pesticides, and chlorinated solvents. For the purpos... Read More about Biological tools to assess contaminant bioavailability in soils.

Microbial metabolism of xenobiotics: Fundamental and applied research (1994)
Journal Article
Singleton, I. (1994). Microbial metabolism of xenobiotics: Fundamental and applied research. Journal of Chemical Technology and Biotechnology, 59(1), 9-23. doi:10.1002/jctb.280590104

The ability of microorganisms to metabolise xenobiotic compounds has received much attention due to the environmental persistence and toxicity of these chemicals. The microbial degradation of xenobiotics is seen as a cost effective method of removing... Read More about Microbial metabolism of xenobiotics: Fundamental and applied research.

Quantitative Use of Fluorescent In Situ Hybridization To Examine Relationships between Mycolic Acid-Containing Actinomycetes and Foaming in Activated Sludge Plants (2000)
Journal Article
Davenport, R. J., Curtis, T. P., Goodfellow, M., Stainsby, F. M., & Bingley, M. (2000). Quantitative Use of Fluorescent In Situ Hybridization To Examine Relationships between Mycolic Acid-Containing Actinomycetes and Foaming in Activated Sludge Plants. Applied and Environmental Microbiology, 66(3), 1158-1166. https://doi.org/10.1128/aem.66.3.1158-1166.2000

The formation of viscous foams on aeration basins and secondary clarifiers of activated sludge plants is a common and widespread problem. Foam formation is often attributed to the presence of mycolic acid-containing actinomycetes (mycolata). In order... Read More about Quantitative Use of Fluorescent In Situ Hybridization To Examine Relationships between Mycolic Acid-Containing Actinomycetes and Foaming in Activated Sludge Plants.

Measuring environmental contamination in critical care using dilute hydrogen peroxide (DHP) technology: An observational cross-over study (2020)
Journal Article
Oon, A., Reading, E., Ferguson, J., Dancer, S., & Mitchell, B. (2020). Measuring environmental contamination in critical care using dilute hydrogen peroxide (DHP) technology: An observational cross-over study. Infection, Disease & Health, 25(2), 107-112. https://doi.org/10.1016/j.idh.2019.12.005

Background The environment has an important role in the transmission of healthcare associated infections. This has encouraged interest in novel methods to improve hygiene in hospitals. One such technology is the use of hydrogen peroxide to decontami... Read More about Measuring environmental contamination in critical care using dilute hydrogen peroxide (DHP) technology: An observational cross-over study.

Dos and don’ts for hospital cleaning (2016)
Journal Article
Dancer, S. J. (2016). Dos and don’ts for hospital cleaning. Current Opinion in Infectious Diseases, 29(4), 415-423. https://doi.org/10.1097/qco.0000000000000289

Purpose of review: More evidence is emerging on the role of cleaning and decontamination for reducing hospital-acquired infection. Timely and adequate removal of environmental pathogens leads to measurable clinical benefits for patients. This article... Read More about Dos and don’ts for hospital cleaning.

Hand antisepsis without decreasing efficacy by shortening the rub-in time of alcohol-based handrubs to 15 seconds (2019)
Journal Article
Harnoss, J., Dancer, S., Kaden, C., Baguhl, R., Kohlmann, T., Papke, R., Zygmunt, M., Assadian, O., Suchomel, M., Pittet, D., & Kramer, A. (2020). Hand antisepsis without decreasing efficacy by shortening the rub-in time of alcohol-based handrubs to 15 seconds. Journal of Hospital Infection, 104(4), 419-424. https://doi.org/10.1016/j.jhin.2019.09.004

Background A previous study among neonatal intensive care unit (NICU) nurses showed that the antibacterial efficacy of alcohol-based handrubs (ABHR) can be achieved in 15 s instead of 30 s with a significant increase in the frequency of hand antisep... Read More about Hand antisepsis without decreasing efficacy by shortening the rub-in time of alcohol-based handrubs to 15 seconds.

The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK (2019)
Journal Article
Hooper, K. M., Casanova, V., Kemp, S., Staines, K. A., Satsangi, J., Barlow, P. G., Henderson, P., & Stevens, C. (2019). The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK. Inflammatory Bowel Diseases, 25(9), 1481-1496. https://doi.org/10.1093/ibd/izz039

Background Genetic studies have strongly linked autophagy to Crohn's disease (CD) and stimulating autophagy in CD patients may be therapeutically beneficial. The aim of this study was to evaluate the effect of current inflammatory bowel disease (IBD)... Read More about The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK.

Immunological homeostasis at the ovine placenta may reflect the degree of maternal foetal interaction (2019)
Journal Article
Wattegedera, S. R., Doull, L. E., Goncheva, M. I., Wheelhouse, N., Watson, D. M., Pearce, J., Benavides, J., Palarea-Albaladejo, J., Mcinnes, C. J., Ballingall, K., & Entrican, G. (2019). Immunological homeostasis at the ovine placenta may reflect the degree of maternal foetal interaction. Frontiers in Immunology, 9, https://doi.org/10.3389/fimmu.2018.03025

Successful mammalian pregnancies are a result of complex physiological, endocrinological and immunological processes that combine to create an environment where the mother is tolerant to the semi-allogeneic fetus. Our knowledge of the mechanisms that... Read More about Immunological homeostasis at the ovine placenta may reflect the degree of maternal foetal interaction.

Ferlavirus-related deaths in a collection of viperid snakes (2018)
Journal Article
Flach, E. J., Dagleish, M. P., Feltrer, Y., Gill, I. S., Marschang, R. E., Masters, N., Orós, J., Pocknell, A., Rendle, M. E., Strike, T., Tapley, B., & Wheelhouse, N. M. (2018). Ferlavirus-related deaths in a collection of viperid snakes. Journal of Zoo and Wildlife Medicine, 49(4), 983-995. https://doi.org/10.1638/2017-0223.1

Between June and October 2013 26 snakes of six viperid species kept in two adjoining rooms died (n = 16) or were euthanized on medical (1) or welfare grounds (9). Two were from the main zoo collection, but the other 24 had been imported and quarantin... Read More about Ferlavirus-related deaths in a collection of viperid snakes.

Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease (2018)
Journal Article
Hooper, K. M., Barlow, P. G., Henderson, P., & Stevens, C. (2019). Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease. Inflammatory Bowel Diseases, 25(4), 661-671. https://doi.org/10.1093/ibd/izy380

Inflammatory Bowel Disease (IBD), which includes Crohn's disease (CD) and ulcerative colitis, is characterised by chronic inflammation of the gastrointestinal tract. Aetiology involves a combination of genetic and environmental factors resulting in a... Read More about Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease.

Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain (2017)
Journal Article
Velasova, M., Damaso, A., Prakashbabu, B. C., Gibbons, J., Wheelhouse, N., Longbottom, D., Van Winden, S., Green, M., & Guitian, J. (2017). Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain. Journal of Dairy Science, 100(11), 9215-9233. https://doi.org/10.3168/jds.2016-11863

To implement appropriate and effective disease control programs at national level, up-to-date and unbiased information on disease frequency is needed. The aim of this study was to estimate the prevalence of selected endemic infectious diseases in th... Read More about Herd-level prevalence of selected endemic infectious diseases of dairy cows in Great Britain.

Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS (2017)
Journal Article
Livingstone, M., Wheelhouse, N., Ensor, H., Rocchi, M., Maley, S., Aitchison, K., Wattegedera, S., Wilson, K., Sait, M., Siarkou, V., Vretou, E., Entrican, G., Dagleish, M., & Longbottom, D. (2017). Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS. PLOS ONE, 12(5), Article e0177653. https://doi.org/10.1371/journal.pone.0177653

This study investigated the pathogenesis of two variant strains (LLG and POS) of Chlamydia abortus, in comparison to a typical wild-type strain (S26/3) which is known to be responsible for late term abortion in small ruminants. Challenge with the thr... Read More about Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS.

Estimating excess length of stay due to healthcare-associated infections: A systematic review and meta-analysis of statistical methodology (2018)
Journal Article
Manoukian, S., Stewart, S., Dancer, S., Graves, N., Mason, H., McFarland, A., Robertson, C., & Reilly, J. (2018). Estimating excess length of stay due to healthcare-associated infections: A systematic review and meta-analysis of statistical methodology. Journal of Hospital Infection, https://doi.org/10.1016/j.jhin.2018.06.003

Background Healthcare-associated infection (HAI) affects millions of patients worldwide. HAI is associated with increased healthcare costs, owing primarily to increased hospital length of stay (LOS) but calculating these costs is complicated due to... Read More about Estimating excess length of stay due to healthcare-associated infections: A systematic review and meta-analysis of statistical methodology.