R. J. Davenport
Quantitative Use of Fluorescent In Situ Hybridization To Examine Relationships between Mycolic Acid-Containing Actinomycetes and Foaming in Activated Sludge Plants
Davenport, R. J.; Curtis, T. P.; Goodfellow, M.; Stainsby, F. M.; Bingley, M.
Authors
Abstract
The formation of viscous foams on aeration basins and secondary clarifiers of activated sludge plants is a common and widespread problem. Foam formation is often attributed to the presence of mycolic acid-containing actinomycetes (mycolata). In order to examine the relationship between the number of mycolata and foam, we developed a group-specific probe targeting the 16S rRNA of the mycolata, a protocol to permeabilize mycolata, and a statistically robust quantification method. Statistical analyses showed that a lipase-based permeabilization method was quantitatively superior to previously described methods (P
Citation
Davenport, R. J., Curtis, T. P., Goodfellow, M., Stainsby, F. M., & Bingley, M. (2000). Quantitative Use of Fluorescent In Situ Hybridization To Examine Relationships between Mycolic Acid-Containing Actinomycetes and Foaming in Activated Sludge Plants. Applied and Environmental Microbiology, 66(3), 1158-1166. https://doi.org/10.1128/aem.66.3.1158-1166.2000
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 30, 1999 |
Publication Date | Mar 1, 2000 |
Deposit Date | Aug 5, 2016 |
Journal | Applied and Environmental Microbiology |
Print ISSN | 0099-2240 |
Electronic ISSN | 1098-5336 |
Publisher | American Society for Microbiology |
Peer Reviewed | Peer Reviewed |
Volume | 66 |
Issue | 3 |
Pages | 1158-1166 |
DOI | https://doi.org/10.1128/aem.66.3.1158-1166.2000 |
Keywords | Biotechnology; Food Science; Ecology; Applied Microbiology and Biotechnology |
Public URL | http://researchrepository.napier.ac.uk/Output/328755 |
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