Quantitative Use of Fluorescent In Situ Hybridization To Examine Relationships between Mycolic Acid-Containing Actinomycetes and Foaming in Activated Sludge Plants

Davenport, R. J.; Curtis, T. P.; Goodfellow, M.; Stainsby, F. M.; Bingley, M.

Abstract

The formation of viscous foams on aeration basins and secondary clarifiers of activated sludge plants is a common and widespread problem. Foam formation is often attributed to the presence of mycolic acid-containing actinomycetes (mycolata). In order to examine the relationship between the number of mycolata and foam, we developed a group-specific probe targeting the 16S rRNA of the mycolata, a protocol to permeabilize mycolata, and a statistically robust quantification method. Statistical analyses showed that a lipase-based permeabilization method was quantitatively superior to previously described methods (P

Citation

Davenport, R. J., Curtis, T. P., Goodfellow, M., Stainsby, F. M., & Bingley, M. (2000). Quantitative Use of Fluorescent In Situ Hybridization To Examine Relationships between Mycolic Acid-Containing Actinomycetes and Foaming in Activated Sludge Plants. Applied and Environmental Microbiology, 66(3), 1158-1166. https://doi.org/10.1128/aem.66.3.1158-1166.2000

• Journal Article Type: Article
• Acceptance Date: Nov 30, 1999
• Publication Date: Mar 1, 2000
• Deposit Date: Aug 5, 2016
• Journal: Applied and Environmental Microbiology
• Print ISSN: 0099-2240
• Electronic ISSN: 1098-5336
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 66
• Issue: 3
• Pages: 1158-1166
• DOI: https://doi.org/10.1128/aem.66.3.1158-1166.2000
• Keywords: Biotechnology; Food Science; Ecology; Applied Microbiology and Biotechnology
• Public URL: http://researchrepository.napier.ac.uk/Output/328755