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Outputs (39)

Evolution and immunopathology of chikungunya virus informs therapeutic development (2023)
Journal Article
Henderson Sousa, F., Ghaisani Komarudin, A., Findlay-Greene, F., Bowolaksono, A., Sasmono, R. T., Stevens, C., & Barlow, P. G. (2023). Evolution and immunopathology of chikungunya virus informs therapeutic development. Disease Models and Mechanisms, 16(4), https://doi.org/10.1242/dmm.049804

Chikungunya virus (CHIKV), a mosquito-borne alphavirus, is an emerging global threat identified in more than 60 countries across continents. The risk of CHIKV transmission is rising due to increased global interactions, year-round presence of mosquit... Read More about Evolution and immunopathology of chikungunya virus informs therapeutic development.

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) (2021)
Journal Article
Klionsky, D. J., Kamal Abdel-Aziz, A., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Stevens, C., & et al. (2021). Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy, 17(1), 1-382. https://doi.org/10.1080/15548627.2020.1797280

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have als... Read More about Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition).

Models of osteoarthritis: relevance and new insights (2020)
Journal Article
Samvelyan, H., Hughes, D., Stevens, C., & Staines, K. A. (2021). Models of osteoarthritis: relevance and new insights. Calcified Tissue International, 109(3), 243-256. https://doi.org/10.1007/s00223-020-00670-x

Osteoarthritis (OA) is a progressive and disabling musculoskeletal disease affecting millions of people and resulting in major healthcare costs worldwide. It is the most common form of arthritis, characterised by degradation of the articular cartilag... Read More about Models of osteoarthritis: relevance and new insights.

Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection (2020)
Journal Article
Casanova, V., Sousa, F. H., Shakamuri, P., Svoboda, P., Buch, C., D'Acremont, M., Christophorou, M. A., Pohl, J., Stevens, C., & Barlow, P. G. (2020). Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection. Frontiers in Immunology, 11, Article 85. https://doi.org/10.3389/fimmu.2020.00085

Human rhinoviruses (HRV) are the most common cause of viral respiratory tract infections. While normally mild and self-limiting in healthy adults, HRV infections are associated with bronchiolitis in infants, pneumonia in immunocompromised patients, a... Read More about Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection.

Design of a New Peptide Substrate Probe of the Putative Biomarker Legumain with Potential Application in Prostate Cancer Diagnosis ex vivo (2019)
Journal Article
Mathur, S., Turnbull, A., Akaev, I., Stevens, C., Agrawal, N., Chopra, M., & Mincher, D. (2020). Design of a New Peptide Substrate Probe of the Putative Biomarker Legumain with Potential Application in Prostate Cancer Diagnosis ex vivo. International Journal of Peptide Research and Therapeutics, 26, 1965-1980. https://doi.org/10.1007/s10989-019-09994-1

The lysosomal endoprotease legumain (asparaginyl endoprotease) has been proposed as a putative biomarker in prostate tumours, in which the enzyme is markedly overexpressed. Overexpression, coupled with highly selective specificity for cleavage of sub... Read More about Design of a New Peptide Substrate Probe of the Putative Biomarker Legumain with Potential Application in Prostate Cancer Diagnosis ex vivo.

Azathioprine Has a Deleterious Effect on the Bone Health of Mice with DSS-Induced Inflammatory Bowel Disease (2019)
Journal Article
Morgan, S., Hooper, K. M., Milne, E. M., Farquharson, C., Stevens, C., & Staines, K. A. (2019). Azathioprine Has a Deleterious Effect on the Bone Health of Mice with DSS-Induced Inflammatory Bowel Disease. International Journal of Molecular Sciences, 20(23), Article 6085. https://doi.org/10.3390/ijms20236085

Patients with inflammatory bowel disease (IBD) often present poor bone health and are 40% more at risk of bone fracture. Studies have implicated autophagy in IBD pathology and drugs used to treat IBD stimulate autophagy in varying degrees, however, t... Read More about Azathioprine Has a Deleterious Effect on the Bone Health of Mice with DSS-Induced Inflammatory Bowel Disease.

Regulation of the expression of DAPK1 by SUMO pathway. (2019)
Journal Article
Wang, Q., Zhang, X., Chen, L., Weng, S., Xia, Y., Ye, Y., …Lin, Y. (2019). Regulation of the expression of DAPK1 by SUMO pathway. Biomolecules, 9(4), 151. https://doi.org/10.3390/biom9040151

Death Associated Protein Kinase 1 (DAPK1) is an important signaling kinase mediating the biological e ect of multiple natural biomolecules such as IFN-, TNF- , curcumin, etc. DAPK1 is degraded through both ubiquitin-proteasomal and lysosomal degradat... Read More about Regulation of the expression of DAPK1 by SUMO pathway..

The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK (2019)
Journal Article
Hooper, K. M., Casanova, V., Kemp, S., Staines, K. A., Satsangi, J., Barlow, P. G., Henderson, P., & Stevens, C. (2019). The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK. Inflammatory Bowel Diseases, 25(9), 1481-1496. https://doi.org/10.1093/ibd/izz039

Background Genetic studies have strongly linked autophagy to Crohn's disease (CD) and stimulating autophagy in CD patients may be therapeutically beneficial. The aim of this study was to evaluate the effect of current inflammatory bowel disease (IBD)... Read More about The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK.

The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy (2019)
Journal Article
Biskou, O., Casanova, V., Hooper, K., Kemp, S., Wright, G. P., Satsangi, J., Barlow, P., & Stevens, C. (2019). The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy. PLOS ONE, 14(1), Article e0209665. https://doi.org/10.1371/journal.pone.0209665

The cytoskeletal protein vimentin plays a key role in positioning of organelles within the cytosol and has been linked to the regulation of numerous cellular processes including autophagy, however, how vimentin regulates autophagy remains relatively... Read More about The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy.

Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease (2018)
Journal Article
Hooper, K. M., Barlow, P. G., Henderson, P., & Stevens, C. (2019). Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease. Inflammatory Bowel Diseases, 25(4), 661-671. https://doi.org/10.1093/ibd/izy380

Inflammatory Bowel Disease (IBD), which includes Crohn's disease (CD) and ulcerative colitis, is characterised by chronic inflammation of the gastrointestinal tract. Aetiology involves a combination of genetic and environmental factors resulting in a... Read More about Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease.

Antiviral therapeutic approaches for human rhinovirus infections (2018)
Journal Article
Casanova, V., Sousa, F. H., Stevens, C., & Barlow, P. G. (2018). Antiviral therapeutic approaches for human rhinovirus infections. Future Virology, https://doi.org/10.2217/fvl-2018-0016

Human rhinoviruses (RV) are the primary etiological agent of the common cold. This infection can be mild and self-limiting in immunocompetent hosts, but can be associated with bronchiolitis in infants, pneumonia in the immunosuppressed, and exacerba... Read More about Antiviral therapeutic approaches for human rhinovirus infections.

Cathelicidins display conserved direct antiviral activity towards rhinovirus. (2017)
Journal Article
Sousa, F. H., Casanova, V., Findlay, F., Stevens, C., Svoboda, P., Pohl, J., Proudfoot, L., & Barlow, P. G. (2017). Cathelicidins display conserved direct antiviral activity towards rhinovirus. Peptides, 95, 76-83. https://doi.org/10.1016/j.peptides.2017.07.013

Human rhinoviruses (HRVs) are the most common cause of viral respiratory tract infections, and are associated with significant morbidity and mortality in immunocompromised individuals and patients with pre-existing pulmonary conditions. The therapeut... Read More about Cathelicidins display conserved direct antiviral activity towards rhinovirus..

Design and evaluation of novel theranostic fluorogenic dual probe-prodrug in cancer (2016)
Presentation / Conference Contribution
Mathur, S., Mincher, D., Turnbull, A., Stevens, C., & Poole, A. (2016). Design and evaluation of novel theranostic fluorogenic dual probe-prodrug in cancer. European Journal of Cancer, 61, S142. https://doi.org/10.1016/s0959-8049%2816%2961501-0

Background: In spite of major advances in the diagnosis and treatment of cancer, there remains a paucity of biomarkers for early detection. Legumain is a potential cancer biomarker and a molecular target for imaging and drug targeting. Legumain is a... Read More about Design and evaluation of novel theranostic fluorogenic dual probe-prodrug in cancer.

Inflammatory Bowel Disease Drugs: A Focus on Autophagy (2016)
Journal Article
Hooper, K. M., Barlow, P. G., Stevens, C., & Henderson, P. (2017). Inflammatory Bowel Disease Drugs: A Focus on Autophagy. Journal of Crohn's and Colitis, 11(1), 118-127. https://doi.org/10.1093/ecco-jcc/jjw127

Inflammatory bowel disease [IBD] is characterized by chronic inflammation of the gastrointestinal tract. Medications such as corticosteroids, thiopurines, immunomodulators and biologic agents are used to induce and maintain remission; however, respon... Read More about Inflammatory Bowel Disease Drugs: A Focus on Autophagy.

Cationic host defense peptides; novel antimicrobial therapeutics against Category A pathogens and emerging infections (2016)
Journal Article
Findlay, F., Proudfoot, L., Stevens, C., & Barlow, P. G. (2016). Cationic host defense peptides; novel antimicrobial therapeutics against Category A pathogens and emerging infections. Pathogens and Global Health, 110(4-5), 137-147. https://doi.org/10.1080/20477724.2016.1195036

Cationic Host Defense Peptides (HDP, also known as antimicrobial peptides) are crucial components of the innate immune system and possess broad-spectrum antibacterial, antiviral, and immunomodulatory activities. They can contribute to the rapid clear... Read More about Cationic host defense peptides; novel antimicrobial therapeutics against Category A pathogens and emerging infections.

Antiviral host defence peptides. (2016)
Book Chapter
Sousa, F. H., Casanova, V., Stevens, C., & Barlow, P. G. (2016). Antiviral host defence peptides. In R. M. Epand (Ed.), Host Defense Peptides and Their Potential as Therapeutic Agents (57-94). Springer. https://doi.org/10.1007/978-3-319-32949-9_3

The on going global mortality and morbidity associated with viral pathogens highlights the need for the continued development of effective, novel antiviral molecules. The antiviral activity of cationic host defence peptides is of significant interest... Read More about Antiviral host defence peptides..

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). (2016)
Journal Article
Baghdiguian, S., Bagniewska-Zadworna, A., Baek, S., Baehrecke, E. H., Bae, S. H., Bae, D., Bae, J., Backer, J. M., Azad, N., Bachetti, T., Awale, S., Avantaggiati, M. L., Avrahami, L., Avagliano, L., Autelli, R., Auburger, G., Aurelian, L., Auberger, P., Attardi, L. D., Atherton, S. S., …Stevens, C. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12(1), 1-222. https://doi.org/10.1080/15548627.2015.1100356

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies... Read More about Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)..

PWE-090 the effect of commonly used Ibd drugs on autophagy induction using an in vitro cell culture system. (2014)
Journal Article
Henderson, P., Satsangi, J., Wilson, D., & Stevens, C. (2014). PWE-090 the effect of commonly used Ibd drugs on autophagy induction using an in vitro cell culture system. Gut, 63(Suppl 1), A163. https://doi.org/10.1136/gutjnl-2014-307263.350

Introduction Genome wide association studies and functional experiments in inflammatory bowel disease (IBD) have delineated the importance of autophagy in IBD pathogenesis. We aimed to determine the effect of commonly utilised IBD drugs on autophagy... Read More about PWE-090 the effect of commonly used Ibd drugs on autophagy induction using an in vitro cell culture system..

Autophagic targeting of Src promotes cancer cell survival following reduced FAK signalling (2012)
Journal Article
Sandilands, E., Serrels, B., McEwan, D. G., Morton, J. P., Macagno, J. P., McLeod, K., Stevens, C., Brunton, V. G., Langdon, W. Y., Vidal, M., Sansom, O. J., Dikic, I., Wilkinson, S., & Frame, M. C. (2012). Autophagic targeting of Src promotes cancer cell survival following reduced FAK signalling. Nature Cell Biology, 14(1), 51-60. https://doi.org/10.1038/ncb2386

Here we describe a mechanism that cancer cells use to survive when flux through the Src/FAK pathway is severely perturbed. Depletion of FAK, detachment of FAK-proficient cells or expression of non-phosphorylatable FAK proteins causes sequestration of... Read More about Autophagic targeting of Src promotes cancer cell survival following reduced FAK signalling.

A role for vimentin in Crohn disease (2012)
Journal Article
Henderson, P., Wilson, D. C., Satsangi, J., & Stevens, C. (2012). A role for vimentin in Crohn disease. Autophagy, 8(11), 1695-1696. https://doi.org/10.4161/auto.21690

Crohn disease (CD), one of the major chronic inflammatory bowel diseases, occurs anywhere in the gastrointestinal tract with discontinuous transmural inflammation. A number of studies have now demonstrated that genetic predisposition, environmental... Read More about A role for vimentin in Crohn disease.

The Role of Autophagy in Crohn’s Disease (2012)
Journal Article
Henderson, P., & Stevens, C. (2012). The Role of Autophagy in Crohn’s Disease. Cells, 1(3), 492-519. https://doi.org/10.3390/cells1030492

(Macro)-autophagy is a homeostatic process by which eukaryotic cells dispose of protein aggregates and damaged organelles. Autophagy is also used to degrade micro-organisms that invade intracellularly in a process termed xenophagy. Genome-wide associ... Read More about The Role of Autophagy in Crohn’s Disease.

The intermediate filament protein, vimentin, is a regulator of NOD2 activity (2012)
Journal Article
Stevens, C., Henderson, P., Nimmo, E. R., Soares, D. C., Dogan, B., Simpson, K. W., Barrett, J. C., Wilson, D. C., & Satsangi, J. (2013). The intermediate filament protein, vimentin, is a regulator of NOD2 activity. Gut, 62(5), 695-707. https://doi.org/10.1136/gutjnl-2011-301775

Objective Mutations in the nucleotide-binding oligomerisation domain-containing protein 2 (NOD2) gene remain the strongest genetic determinants for Crohn's disease (CD). Having previously identified vimentin as a novel NOD2-interacting protein, the a... Read More about The intermediate filament protein, vimentin, is a regulator of NOD2 activity.

TLE1 modifies the effects of NOD2 in the pathogenesis of Crohn's Disease (2011)
Journal Article
Nimmo, E. R., Stevens, C., Phillips, A. M., Smith, A., Drummond, H. E., Noble, C. L., Quail, M., Davies, G., Aldhous, M. C., Wilson, D. C., & Satsangi, J. (2011). TLE1 modifies the effects of NOD2 in the pathogenesis of Crohn's Disease. Gastroenterology, 141(3), 972-981.e2. https://doi.org/10.1053/j.gastro.2011.05.043

Background & Aims The mechanisms by which specific mutations in NOD2/CARD15 increase the risk for Crohn's disease (CD) are unclear. We identified proteins that interact with {NOD2} and investigated them by expression, genetic, and functional analyses... Read More about TLE1 modifies the effects of NOD2 in the pathogenesis of Crohn's Disease.

Tuberous sclerosis-2 (TSC2) regulates the stability of death-associated protein kinase-1 (DAPK) through a lysosome-dependent degradation pathway (2010)
Journal Article
Hupp, T., Lin, Y., Henderson, P., Pettersson, S., Satsangi, J., Hupp, T. R., & Stevens, C. (2010). Tuberous sclerosis-2 (TSC2) regulates the stability of death-associated protein kinase-1 (DAPK) through a lysosome-dependent degradation pathway. FEBS Journal, 278(2), 354-370. https://doi.org/10.1111/j.1742-4658.2010.07959.x

We previously identified a novel interaction between tuberous sclerosis-2 (TSC2) and death-associated protein kinase-1 (DAPK), the consequence being that DAPK catalyses the inactivating phosphorylation of TSC2 to stimulate mammalian target of rapamyc... Read More about Tuberous sclerosis-2 (TSC2) regulates the stability of death-associated protein kinase-1 (DAPK) through a lysosome-dependent degradation pathway.

Peptide Combinatorial Libraries Identify TSC2 as a Death-associated Protein Kinase (DAPK) Death Domain-binding Protein and Reveal a Stimulatory Role for DAPK in mTORC1 Signaling (2009)
Journal Article
Stevens, C., Lin, Y., Harrison, B., Burch, L., Ridgway, R. A., Sansom, O. J., & Hupp, T. R. (2009). Peptide Combinatorial Libraries Identify TSC2 as a Death-associated Protein Kinase (DAPK) Death Domain-binding Protein and Reveal a Stimulatory Role for DAPK in mTORC1 Signaling. Journal of Biological Chemistry, 284, 334-344. https://doi.org/10.1074/jbc.M805165200

Death-associated protein kinase (DAPK) is a multidomain enzyme that plays a central role in autophagic and apoptotic signaling, although the protein-protein interactions regulating DAPK functions are not well defined. Peptide aptamer libraries were u... Read More about Peptide Combinatorial Libraries Identify TSC2 as a Death-associated Protein Kinase (DAPK) Death Domain-binding Protein and Reveal a Stimulatory Role for DAPK in mTORC1 Signaling.

Death‐associated protein kinase (DAPK) and signal transduction: blebbing in programmed cell death. (2009)
Journal Article
Bovellan, M., Fritzsche, M., Stevens, C., & Charras, G. (2010). Death‐associated protein kinase (DAPK) and signal transduction: blebbing in programmed cell death. FEBS Journal, 277(1), 58-65. https://doi.org/10.1111/j.1742-4658.2009.07412.x

Death-associated protein kinase (DAPK) is a stress-regulated protein kinase that mediates a range of processes, including signal-induced cell death and autophagy. Although the kinase domain of DAPK has a range of substrates that mediate its signallin... Read More about Death‐associated protein kinase (DAPK) and signal transduction: blebbing in programmed cell death..

Death-associated protein kinase (DAPK) and signal transduction: additional roles beyond cell death: DAPK and signal transduction (2009)
Journal Article
Lin, Y., Hupp, T. R., & Stevens, C. (2010). Death-associated protein kinase (DAPK) and signal transduction: additional roles beyond cell death: DAPK and signal transduction. FEBS Journal, 277(1), 48-57. https://doi.org/10.1111/j.1742-4658.2009.07411.x

Death-associated protein kinase (DAPK) is a stress-regulated protein kinase that mediates a range of processes, including signal-induced cell death and autophagy. Although the kinase domain of DAPK has a range of substrates that mediate its signalli... Read More about Death-associated protein kinase (DAPK) and signal transduction: additional roles beyond cell death: DAPK and signal transduction.

Autophagy: from basic science to clinical application (2009)
Journal Article
Van Limbergen, J., Stevens, C., Nimmo, E. R., Wilson, D. C. & Satsangi, J. (2009). Autophagy: from basic science to clinical application. Mucosal immunology. 2. (4). 315-330. doi:10.1038/mi.2009.20. ISSN 1933-0219.

Autophagy is a cellular pathway involved in protein and organelle degradation, which is likely to represent an innate adaptation to starvation. In times of nutrient deficiency, the cell can self-digest and recycle some nonessential components through... Read More about Autophagy: from basic science to clinical application.

The alternative splice variant of DAPK-1, s-DAPK-1, induces proteasome-independent DAPK-1 destabilization. (2009)
Journal Article
Lin, Y., Stevens, C., Harrison, B., Pathuri, S., Amin, E., & Hupp, T. R. (2009). The alternative splice variant of DAPK-1, s-DAPK-1, induces proteasome-independent DAPK-1 destabilization. Molecular and Cellular Biochemistry, 328(1-2), 101-107. https://doi.org/10.1007/s11010-009-0079-4

Death-associated protein kinase 1 (DAPK-1) is a Ca2+/CaM-regulated kinase involved in multiple cellular signalling pathways that trigger cell survival, apoptosis, and autophagy. An alternatively spliced product expressed from the dapk1 locus, named s... Read More about The alternative splice variant of DAPK-1, s-DAPK-1, induces proteasome-independent DAPK-1 destabilization..

ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1: ATP stimulated inhibition of E2F1 by MDM2 (2008)
Journal Article
Stevens, C., Pettersson, S., Wawrzynow, B., Wallace, M., Ball, K., Zylicz, A., & Hupp, T. R. (2008). ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1: ATP stimulated inhibition of E2F1 by MDM2. FEBS Journal, 275(19), 4875-4886. https://doi.org/10.1111/j.1742-4658.2008.06627.x

Murine double minute 2 (MDM2) protein exhibits many diverse biochemical functions on the tumour suppressor protein p53, including transcriptional suppression and E3 ubiquitin ligase activity. However, more recent data have shown that MDM2 can exhibit... Read More about ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1: ATP stimulated inhibition of E2F1 by MDM2.

An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing: Functional transcript expressed by DAPK-1 locus (2008)
Journal Article
Lin, Y., Stevens, C., Hrstka, R., Harrison, B., Fourtouna, A., Pathuri, S., Vojtesek, B., & Hupp, T. (2008). An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing: Functional transcript expressed by DAPK-1 locus. FEBS Journal, 275(10), 2574-2584. https://doi.org/10.1111/j.1742-4658.2008.06404.x

Death-associated protein kinase 1 (DAPK-1) is a multidomain protein kinase with diverse roles in autophagic, apoptotic and survival pathways. Bioinformatic screens were used to identify a small internal mRNA from the DAPK-1 locus (named s-DAPK-1). Th... Read More about An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing: Functional transcript expressed by DAPK-1 locus.

Novel insights into DAPK autophagic signalling using peptide aptamer combinatorial protein-interaction screens (2008)
Journal Article
Stevens, C. & Hupp, T. R. (2008). Novel insights into DAPK autophagic signalling using peptide aptamer combinatorial protein-interaction screens. Autophagy. 4. (4). 531-533. doi:10.4161/auto.5940. ISSN 1554-8627.

DAPK represents a relatively unique enzyme in the protein kinase superfamily whose major biological functions are linked to both autophagy and signal-mediated apoptosis. However, genetic studies have not yet uncovered how DAPK integrates into the cor... Read More about Novel insights into DAPK autophagic signalling using peptide aptamer combinatorial protein-interaction screens.

DAPK-1 Bbnding to a linear peptide motif in MAP1B stimulates autophagy and membrane blebbing (2008)
Journal Article
Harrison, B., Kraus, M., Burch, L., Stevens, C., Craig, A., Gordon-Weeks, P., & Hupp, T. R. (2008). DAPK-1 Bbnding to a linear peptide motif in MAP1B stimulates autophagy and membrane blebbing. Journal of Biological Chemistry, 283(15), 9999-10014. https://doi.org/10.1074/jbc.M706040200

DAPK-1 (death-activated protein kinase) has wide ranging functions in cell growth control; however, DAPK-1 interacting proteins that mediate these effects are not well defined. Protein-protein interactions are driven in part by linear interaction mot... Read More about DAPK-1 Bbnding to a linear peptide motif in MAP1B stimulates autophagy and membrane blebbing.

Identification of a dominant negative functional domain on DAPK-1 that degrades DAPK-1 protein and stimulates TNFR-1-mediated apoptosis. (2007)
Journal Article
Lin, Y., Stevens, C., & Hupp, T. (2007). Identification of a dominant negative functional domain on DAPK-1 that degrades DAPK-1 protein and stimulates TNFR-1-mediated apoptosis. Journal of Biological Chemistry, 282(23), 16792-16802. https://doi.org/10.1074/jbc.M611559200

DAPK-1 is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. To define mechanisms of DAPK-1 protein regulation, we have determined that DAPK-1 protein has a long half-life... Read More about Identification of a dominant negative functional domain on DAPK-1 that degrades DAPK-1 protein and stimulates TNFR-1-mediated apoptosis..

A Germ Line Mutation in the Death Domain of DAPK-1 Inactivates ERK-induced Apoptosis (2007)
Journal Article
Stevens, C., Lin, Y., Sanchez, M., Amin, E., Copson, E., White, H., Durston, V., Eccles, D. M., & Hupp, T. (2007). A Germ Line Mutation in the Death Domain of DAPK-1 Inactivates ERK-induced Apoptosis. Journal of Biological Chemistry, 282(18), 13791-13803. https://doi.org/10.1074/jbc.m605649200

p53 is activated genetically by a set of kinases that are components of the calcium calmodulin kinase superfamily, including CHK2, AMP kinase, and DAPK-1. In dissecting the mechanism of DAPK-1 control, a novel mutation (N1347S) was identified in the... Read More about A Germ Line Mutation in the Death Domain of DAPK-1 Inactivates ERK-induced Apoptosis.

The emerging role of E2F-1 in the DNA damage response and checkpoint control. (2004)
Journal Article
Stevens, C., & La Thangue, N. B. (2004). The emerging role of E2F-1 in the DNA damage response and checkpoint control. DNA Repair, 3(8-9), 1071-1079. https://doi.org/10.1016/j.dnarep.2004.03.034

Genotoxic stress triggers a myriad of cellular responses including cell cycle arrest, stimulation of {DNA} repair and apoptosis. A central role for the E2F-1 transcription factor in the {DNA} damage response pathway is gaining support. E2F-1 is phosp... Read More about The emerging role of E2F-1 in the DNA damage response and checkpoint control..

A New Role for E2F-1 in Checkpoint Control (2003)
Journal Article
Stevens, C., & Thangue, N. B. L. (2003). A New Role for E2F-1 in Checkpoint Control. Cell Cycle, 2(5), 434-436. https://doi.org/10.4161/cc.2.5.462

In response to DNA damage, E2F-1 is induced and phosphorylated. Phosphorylated E2F-1 can reside in discrete nuclear structures and induce apoptosis, suggesting a unique role for E2F-1 in DNA repair and checkpoint functions.

Chk2 activates E2F-1 in response to DNA damage (2003)
Journal Article
Stevens, C., Smith, L., & La Thangue, N. B. (2003). Chk2 activates E2F-1 in response to DNA damage. Nature Cell Biology, 5(5), 401-409. https://doi.org/10.1038/ncb974

The E2F-1 transcription factor is regulated during cell cycle progression and induced by cellular stress, such as DNA damage. We report that checkpoint kinase 2 (Chk2) regulates E2F-1 activity in response to the DNA-damaging agent etoposide. A Chk2 c... Read More about Chk2 activates E2F-1 in response to DNA damage.

E2F and cell cycle control: a double-edged sword (2003)
Journal Article
Stevens, C., & La Thangue, N. B. (2003). E2F and cell cycle control: a double-edged sword. Archives of Biochemistry and Biophysics, 412(2), 157-169. https://doi.org/10.1016/s0003-9861%2803%2900054-7

The E2F family of transcription factors plays a central role in regulating cellular proliferation by controlling the expression of both the genes required for cell cycle progression, particularly DNA synthesis, and the genes involved with apoptosis.... Read More about E2F and cell cycle control: a double-edged sword.