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A Permeapad assisted sample preparation workflow for the selective determination of anticonvulsants in human serum by liquid chromatography tandem mass spectrometry.

People Involved

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Amy Dillon A.Dillon@napier.ac.uk
Skills Enhancement Tutor

Project Description

Permeapad® 96-well plate (Jacobsen, Nielsen, Brandl, & Bauer-Brandl, 2020) is a high throughput screening tool developed to study the mass diffusion of drugs in the gastrointestinal tract (GIT). The design of the device is similar to the Franz-cell diffusion apparatus, which features a donor and a receiver chamber separated by a membrane (in transdermal permeability diffusion experiments this can be either natural or synthetic). In the Permeapad® 96-well plate, the two chambers are separated by two cellulose-hydrate membranes enclosing a layer of dry phospholipids, mimicking the GIT membranes for the study of high throughput permeation in microscale (combined capacity of both chambers is 400 μL). This device has been successfully used to measure the permeation of compounds and has been validated against absorption data assessed on Caco-2 monolayers (Jacobsen et al., 2020). The results collected using this approach are more reproducible than similar methods, based on PAMPA, and can be conducted more quickly.
This proposal focuses on a novel application of this device as a more selective, more environmentally friendly, and more efficient sample preparation tool for extracting and quantifying anticonvulsant drugs in human serum for the purpose of therapeutic drug monitoring (TDM). Anticonvulsant drugs, e.g. phenytoin or carbamazepine, are known to have a narrow therapeutic index and can be affected by concurrent medications (such as antifungal or antibiotics) or conditions, such as liver dysfunction. Thus, this necessitates a more efficient, green, cost effective and selective sample preparation protocol for use in TDM.

Type of Project P04 - Research Charities and Trusts
Status Project Live
Funder(s) ChromoSoc
Value £2,500.00
Project Dates Jul 1, 2025 - Aug 26, 2025



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