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Outputs (63)

Pregnancy length and health in giant pandas: what can metabolic and urinary endocrine markers unveil? (2023)
Journal Article
Wauters, J., Wilson, K. S., Cools, T., Vancsok, C., Bouts, T., Mulot, B., …Li, D. (2023). Pregnancy length and health in giant pandas: what can metabolic and urinary endocrine markers unveil?. Theriogenology Wild, 3, Article 100063. https://doi.org/10.1016/j.therwi.2023.100063

Mature female giant pandas usually ovulate once a year. This is followed by an obligatory luteal phase, consisting of a long-lasting corpus luteum dormancy phase (CLD; primary increase in progestogens) and a much shorter active luteal phase (AL; seco... Read More about Pregnancy length and health in giant pandas: what can metabolic and urinary endocrine markers unveil?.

Development of an acute ovine model of polycystic ovaries to assess the effect of ovarian denervation (2023)
Journal Article
Duncan, W. C., Nicol, L. M., O’Hare, R., Witherington, J., Miranda, J. A., Campbell, B. K., …Rae, M. T. (2023). Development of an acute ovine model of polycystic ovaries to assess the effect of ovarian denervation. Frontiers in Endocrinology, 14, Article 1285269. https://doi.org/10.3389/fendo.2023.1285269

Introduction: Polycystic ovary syndrome (PCOS) seems to be associated with increased ovarian sympathetic nerve activity and in rodent models of PCOS reducing the sympathetic drive to the ovary, through denervation or neuromodulation, improves ovulati... Read More about Development of an acute ovine model of polycystic ovaries to assess the effect of ovarian denervation.

Hepatic Mitochondrial Dysfunction and Risk of Liver Disease in an Ovine Model of “PCOS Males” (2022)
Journal Article
Siemienowicz, K. J., Filis, P., Thomas, J., Fowler, P. A., Duncan, W. C., & Rae, M. T. (2022). Hepatic Mitochondrial Dysfunction and Risk of Liver Disease in an Ovine Model of “PCOS Males”. Biomedicines, 10(6), Article 1291. https://doi.org/10.3390/biomedicines10061291

First-degree male relatives of polycystic ovary syndrome (PCOS) sufferers can develop metabolic abnormalities evidenced by elevated circulating cholesterol and triglycerides, suggestive of a male PCOS equivalent. Similarly, male sheep overexposed to... Read More about Hepatic Mitochondrial Dysfunction and Risk of Liver Disease in an Ovine Model of “PCOS Males”.

Chlamydia inhibits progesterone receptor mRNA expression in SHT-290 cells (2021)
Journal Article
Brown, M., Rae, M., & Wheelhouse, N. (2021). Chlamydia inhibits progesterone receptor mRNA expression in SHT-290 cells. Reproduction and Fertility, 2(1), 9-11. https://doi.org/10.1530/RAF-20-0069

Chlamydia trachomatis is the most commonly diagnosed sexually transmitted infection in the UK, with over 200,000 positive diagnoses annually. The infection is thought to cause reproductive complications including problems in conceiving a pregnancy th... Read More about Chlamydia inhibits progesterone receptor mRNA expression in SHT-290 cells.

Pubertal FGF21 deficit is central in the metabolic pathophysiology of an ovine model of polycystic ovary syndrome (2021)
Journal Article
Siemienowicz, K. J., Furmanska, K., Filis, P., Talia, C., Thomas, J., Fowler, P. A., …Colin Duncan, W. (2021). Pubertal FGF21 deficit is central in the metabolic pathophysiology of an ovine model of polycystic ovary syndrome. Molecular and Cellular Endocrinology, 525, Article 111196. https://doi.org/10.1016/j.mce.2021.111196

Polycystic ovary syndrome (PCOS), affecting over 10% of women, is associated with insulin resistance, obesity, dyslipidaemia, fatty liver and adipose tissue dysfunction. Its pathogenesis is poorly understood and consequently treatment remains subopti... Read More about Pubertal FGF21 deficit is central in the metabolic pathophysiology of an ovine model of polycystic ovary syndrome.

Early pregnancy maternal progesterone administration alters pituitary and testis function and steroid profile in male fetuses (2020)
Journal Article
Siemienowicz, K. J., Wang, Y., Marečková, M., Nio-Kobayashi, J., Fowler, P. A., Rae, M. T., & Duncan, W. C. (2020). Early pregnancy maternal progesterone administration alters pituitary and testis function and steroid profile in male fetuses. Scientific Reports, 10(1), https://doi.org/10.1038/s41598-020-78976-x

Maternal exposure to increased steroid hormones, including estrogens, androgens or glucocorticoids during pregnancy results in chronic conditions in offspring that manifest in adulthood. Little is known about effects of progesterone administration in... Read More about Early pregnancy maternal progesterone administration alters pituitary and testis function and steroid profile in male fetuses.

Aberrant subcutaneous adipogenesis precedes adult metabolic dysfunction in an ovine model of polycystic ovary syndrome (PCOS) (2020)
Journal Article
Siemienowicz, K. J., Coukan, F., Franks, S., Rae, M. T., & Colin Duncan, W. (2021). Aberrant subcutaneous adipogenesis precedes adult metabolic dysfunction in an ovine model of polycystic ovary syndrome (PCOS). Molecular and Cellular Endocrinology, 519, Article 111042. https://doi.org/10.1016/j.mce.2020.111042

Polycystic ovary syndrome (PCOS) affects over 10% of women. Insulin resistance, elevated free fatty acids (FFAs) and increased adiposity are key factors contributing to metabolic dysfunction in PCOS. We hypothesised that aberrant adipogenesis duri... Read More about Aberrant subcutaneous adipogenesis precedes adult metabolic dysfunction in an ovine model of polycystic ovary syndrome (PCOS).

Insights into manipulating postprandial energy expenditure to manage weight gain in polycystic ovary syndrome (PCOS) (2020)
Journal Article
Siemienowicz, K., Rae, M. T., Howells, F., Anderson, C., Nicol, L. M., Franks, S., & Duncan, W. C. (2020). Insights into manipulating postprandial energy expenditure to manage weight gain in polycystic ovary syndrome (PCOS). iScience, 23(6), https://doi.org/10.1016/j.isci.2020.101164

Women with polycystic ovary syndrome (PCOS) are more likely to be obese and have difficulty in losing weight. They demonstrate an obesity-independent deficit in adaptive energy expenditure. We used a clinically-realistic preclinical model to investig... Read More about Insights into manipulating postprandial energy expenditure to manage weight gain in polycystic ovary syndrome (PCOS).

Dyslipidaemia and altered hepatic function in males - consequences of androgen excess in fetal life (2019)
Journal Article
Siemienowicz, K., Filis, P., Shaw, S., Douglas, A., Thomas, J., Howie, F., …Rae, M. (2019). Dyslipidaemia and altered hepatic function in males - consequences of androgen excess in fetal life. Endocrine abstracts, 63, https://doi.org/10.1530/endoabs.63.P669

Introduction: Adult male offspring of women with PCOS have increased dyslipidaemia, characterised by elevated triglycerides (TG), increased total and LDL-cholesterol (LDL-C), and hyperinsulinaemia. As altered intrauterine endocrine environments can ‘... Read More about Dyslipidaemia and altered hepatic function in males - consequences of androgen excess in fetal life.

Fetal androgen exposure is a determinant of adult male metabolic health (2019)
Journal Article
Siemienowicz, K. J., Filis, P., Shaw, S., Douglas, A., Thomas, J., Mulroy, S., …Rae, M. T. (2019). Fetal androgen exposure is a determinant of adult male metabolic health. Scientific Reports, 9, Article 20195 (2019). https://doi.org/10.1038/s41598-019-56790-4

Androgen signalling is a critical driver of male development. Fetal steroid signalling can be dysregulated by a range of environmental insults and clinical conditions. We hypothesised that poor adult male health was partially attributable to aberrant... Read More about Fetal androgen exposure is a determinant of adult male metabolic health.

Urinary estrogens as a non-invasive biomarker of viable pregnancy in the giant panda (Ailuropoda melanoleuca) (2019)
Journal Article
Wilson, K. S., Wauters, J., Valentine, I., McNeilly, A., Girling, S., Li, R., …Duncan, W. C. (2019). Urinary estrogens as a non-invasive biomarker of viable pregnancy in the giant panda (Ailuropoda melanoleuca). Scientific Reports, 9(1), Article 12772. https://doi.org/10.1038/s41598-019-49288-6

Female giant pandas show complex reproductive traits, being seasonally monoestrus, displaying a variable length embryonic diapause and exhibiting pseudopregnancy. Currently, there is no confirmatory non-invasive biomarker of blastocyst implantation o... Read More about Urinary estrogens as a non-invasive biomarker of viable pregnancy in the giant panda (Ailuropoda melanoleuca).

Urinary specific gravity as an alternative for the normalisation of endocrine metabolite concentrations in giant panda (Ailuropoda melanoleuca) reproductive monitoring (2018)
Journal Article
Wauters, J., Wilson, K. S., Bouts, T., Valentine, I., Vanderschueren, K., Ververs, C., …Vanhaecke, L. (2018). Urinary specific gravity as an alternative for the normalisation of endocrine metabolite concentrations in giant panda (Ailuropoda melanoleuca) reproductive monitoring. PLOS ONE, 13(7), Article e0201420. https://doi.org/10.1371/journal.pone.0201420

Reproductive monitoring for captive breeding in giant pandas is based on behavioural observation and non-invasive hormone analysis. In urine, interpretation of results requires normalisation due to an animal’s changing hydration. Correction of urinar... Read More about Urinary specific gravity as an alternative for the normalisation of endocrine metabolite concentrations in giant panda (Ailuropoda melanoleuca) reproductive monitoring.

Preferential activation of HIF-2α adaptive signalling in neuronal-like cells in response to acute hypoxia (2017)
Journal Article
Martín-Aragón Baudel, M. A. S., Rae, M. T., Darlison, M. G., Poole, A. V., & Fraser, J. A. (2017). Preferential activation of HIF-2α adaptive signalling in neuronal-like cells in response to acute hypoxia. PLOS ONE, 12(10), Article e0185664. https://doi.org/10.1371/journal.pone.0185664

Stroke causes severe neuronal damage as disrupted cerebral blood flow starves neurons of oxygen and glucose. The hypoxia inducible factors (HIF-1α and HIF-2α) orchestrate oxygen homeostasis and regulate specific aspects of hypoxic adaptation. Here we... Read More about Preferential activation of HIF-2α adaptive signalling in neuronal-like cells in response to acute hypoxia.

Preferential activation of HIF-2 adaptive mechanisms in neuronal-like cells in response to hypoxia (2017)
Presentation / Conference
Fraser, J., Baudel, M., Rae, M., Darlison, M., & Poole, A. (2017, April). Preferential activation of HIF-2 adaptive mechanisms in neuronal-like cells in response to hypoxia. Poster presented at British Neuroscience Association - festival of neuroscience 2017

Stroke is a leading cause of death and disability worldwide. Blockage, or occlusion, of cerebral arteries causes irreversible neuronal damage as disrupted blood flow starves neurones of oxygen and glucose. The hypoxia inducible factors (HIFs) are mas... Read More about Preferential activation of HIF-2 adaptive mechanisms in neuronal-like cells in response to hypoxia.

The expression of the chloride co-transporters NKCC1 and KCC2 is reversed in the penumbra following (2017)
Conference Proceeding
Baudel, M., Rae, M., Fraser, J., & Poole, A. (2017). The expression of the chloride co-transporters NKCC1 and KCC2 is reversed in the penumbra following. In Brain and Neuroscience: BNA 2017 Festival of Neuroscience: Abstract Book

Stroke is one of the major causes of death and disability worldwide. The harm caused by the interruption of blood flow to the brain unfolds in the subsequent hours and days, so it is critical to identify new therapeutic targets that could reduce neur... Read More about The expression of the chloride co-transporters NKCC1 and KCC2 is reversed in the penumbra following.

Development of in vitro strategies to predict in vivo toxicity of engineered nanoparticles to the male reproductive system. (2016)
Presentation / Conference
Malone, E., Ross, B., Rae, M., Bouwmeester, H., Gosens, I., Cassee, F., …Hutchison, G. (2016, September). Development of in vitro strategies to predict in vivo toxicity of engineered nanoparticles to the male reproductive system. Poster presented at 11th International Particle Toxicology Conference

The introduction of engineered nanomaterials (ENM) to new materials and products requires informed risk assessment. The altered physico-chemical properties of ENM mean traditional assays to evaluate toxicity must be modified or validated to ensure... Read More about Development of in vitro strategies to predict in vivo toxicity of engineered nanoparticles to the male reproductive system..

Ovine trophoblasts express cathelicidin host defence peptide in response to infection (2016)
Journal Article
Coyle, C., Wheelhouse, N., Jacques, M., Longbottom, D., Svoboda, P., Pohl, J., …Barlow, P. G. (2016). Ovine trophoblasts express cathelicidin host defence peptide in response to infection. Journal of Reproductive Immunology, 117, 10-16. https://doi.org/10.1016/j.jri.2016.06.006

Cationic host defence peptides (CHDP; also known as antimicrobial peptides) are key components of the immune response in the female reproductive tract. The role of the placental trophoblast in ovine host defence remains poorly understood. This study... Read More about Ovine trophoblasts express cathelicidin host defence peptide in response to infection.

Developmental programming of polycystic ovary syndrome (PCOS): prenatal androgens establish pancreatic islet α/β cell ratio and subsequent insulin secretion (2016)
Journal Article
Ramaswamy, S., McNeilly, A. S., Rae, M. T., Ramaswamy, S., Grace, C., Mattei, A. A., …Rae, M. T. (2016). Developmental programming of polycystic ovary syndrome (PCOS): prenatal androgens establish pancreatic islet α/β cell ratio and subsequent insulin secretion. Scientific Reports, 6(1), Article 27408. https://doi.org/10.1038/srep27408

Exogenous androgenic steroids applied to pregnant sheep programmes a PCOS-like phenotype in female offspring. Via ultrasound guidance we applied steroids directly to ovine fetuses at d62 and d82 of gestation, and examined fetal (day 90 gestation) and... Read More about Developmental programming of polycystic ovary syndrome (PCOS): prenatal androgens establish pancreatic islet α/β cell ratio and subsequent insulin secretion.

Obesity in PCOS: a consequence of prenatally programmed reduced energy expenditure (2015)
Presentation / Conference
Siemienowicz, K., Rae, M., Lerner, A., Franks, S., & Duncan, C. (2015, July). Obesity in PCOS: a consequence of prenatally programmed reduced energy expenditure. Paper presented at Society for Reproduction and Fertility Annual Conference

Polycystic ovary syndrome, a common endocrine condition affecting up to 10% of women of reproductive age, is associated with an increased risk of developing insulin resistance and obesity. Obesity is associated with increased energy intake or reduced... Read More about Obesity in PCOS: a consequence of prenatally programmed reduced energy expenditure.

In an ovine model of polycystic ovary syndrome (PCOS) prenatal androgens suppress female fetal renal gluconeogenesis. (2015)
Journal Article
Kanellopoulos-Langevin, C., Connolly, F., Rae, M. T., Späth, K., Boswell, L., McNeilly, A. S., & Duncan, W. C. (2015). In an ovine model of polycystic ovary syndrome (PCOS) prenatal androgens suppress female fetal renal gluconeogenesis. PLOS ONE, 10, Article e0132113. https://doi.org/10.1371/journal.pone.0132113

Increased maternal androgen exposure during pregnancy programmes a polycystic ovary syndrome (PCOS)-like condition, with metabolic dysfunction, in adult female offspring. Other in utero exposures associated with the development of insulin resistance,... Read More about In an ovine model of polycystic ovary syndrome (PCOS) prenatal androgens suppress female fetal renal gluconeogenesis..

The local effects of ovarian diathermy in an ovine model of Polycystic Ovary Syndrome (2014)
Journal Article
Shen, W., Connolly, F., Rae, M. T., Butler, M., Klibanov, A. L., Sboros, V., …Duncan, W. C. (2014). The local effects of ovarian diathermy in an ovine model of Polycystic Ovary Syndrome. PLOS ONE, 9, Article e111280. https://doi.org/10.1371/journal.pone.0111280

In order to develop a medical alternative to surgical ovarian diathermy (OD) in polycystic ovary syndrome (PCOS) more mechanistic information is required about OD. We therefore studied the cellular, molecular and vascular effects of diathermy on the... Read More about The local effects of ovarian diathermy in an ovine model of Polycystic Ovary Syndrome.

Waddlia chondrophila Infects and Multiplies in Ovine Trophoblast Cells Stimulating an Inflammatory Immune Response (2014)
Journal Article
Wheelhouse, N., Coyle, C., Barlow, P. G., Mitchell, S., Greub, G., Baszler, T., …Longbottom, D. (2014). Waddlia chondrophila Infects and Multiplies in Ovine Trophoblast Cells Stimulating an Inflammatory Immune Response. PLOS ONE, 9(7), https://doi.org/10.1371/journal.pone.0102386

BACKGROUND Waddlia chondrophila (W. chondrophila) is an emerging abortifacient organism which has been identified in the placentae of humans and cattle. The organism is a member of the order Chlamydiales, and shares many similarities at the genome... Read More about Waddlia chondrophila Infects and Multiplies in Ovine Trophoblast Cells Stimulating an Inflammatory Immune Response.

The pathophysiology of increased hepatic IGF1 expression in an ovine model of polycystic ovary syndrome (2014)
Presentation / Conference
Siemienowicz, K., Boswell, L., Carr, D., Mina, T., Connolly, F., Rae, M., & Duncan, C. (2014, May). The pathophysiology of increased hepatic IGF1 expression in an ovine model of polycystic ovary syndrome. Poster presented at 16th European Congress of Endocrinology

Exposure of pregnant sheep to increased concentrations of testosterone during midgestation results in a PCOS-like condition in the female offspring that includes increased hepatic IGF1. The aim of this study was to investigate the molecular pathophys... Read More about The pathophysiology of increased hepatic IGF1 expression in an ovine model of polycystic ovary syndrome.

Excess Androgens in Utero Alters Fetal Testis Development. (2013)
Journal Article
Connolly, F., Rae, M. T., Bittner, L., Hogg, K., McNeilly, A. S., & Duncan, W. C. (2013). Excess Androgens in Utero Alters Fetal Testis Development. Endocrinology, 154, 1921-1933. https://doi.org/10.1210/en.2012-2153

Prenatal androgenization induces a polycystic ovary syndrome-like phenotype in adult female offspring, which is associated with alterations that can be detected in the fetal ovary, suggesting gestational origins of this condition. We therefore invest... Read More about Excess Androgens in Utero Alters Fetal Testis Development..

The Pancreas Is Altered by In Utero Androgen Exposure: Implications for Clinical Conditions Such as Polycystic Ovary Syndrome (PCOS) (2013)
Journal Article
Franks, S., Rae, M. T., Grace, C., Hogg, K., Wilson, L. M., McHaffie, S. L., …Duncan, C. (2013). The Pancreas Is Altered by In Utero Androgen Exposure: Implications for Clinical Conditions Such as Polycystic Ovary Syndrome (PCOS). PLOS ONE, 8(2), Article e56263. https://doi.org/10.1371/journal.pone.0056263

Using an ovine model of polycystic ovary syndrome (PCOS), (pregnant ewes injected with testosterone propionate (TP) (100 mg twice weekly) from day (d)62 to d102 of d147 gestation (maternal injection – MI-TP)), we previously reported female offspring... Read More about The Pancreas Is Altered by In Utero Androgen Exposure: Implications for Clinical Conditions Such as Polycystic Ovary Syndrome (PCOS).

Maternal undernutrition does not alter Sertoli cell numbers or the expression of key developmental markers in the mid-gestation ovine fetal testis (2013)
Journal Article
Andrade, L. P., Rhind, S. M., Rae, M. T., Kyle, C. E., Jowett, J., & Lea, R. G. (2013). Maternal undernutrition does not alter Sertoli cell numbers or the expression of key developmental markers in the mid-gestation ovine fetal testis. Journal of Negative Results in BioMedicine, 12(1), https://doi.org/10.1186/1477-5751-12-2

Background The aim of this study was to determine the effects of maternal undernutrition on ovine fetal testis morphology and expression of relevant histological indicators. Maternal undernutrition, in sheep, has been reported, previously, to alter... Read More about Maternal undernutrition does not alter Sertoli cell numbers or the expression of key developmental markers in the mid-gestation ovine fetal testis.

Cortisol Inactivation by 11β-Hydroxysteroid dehydrogenase-2 May Enhance Endometrial Angiogenesis via Reduced Thrombospondin-1 in Heavy Menstruation (2009)
Journal Article
Rae, M. T., Mohamad, A., Price, D., Hadoke, P. W. F., Walker, B. R., Mason, J. I., …Critchley, H. O. D. (2009). Cortisol Inactivation by 11β-Hydroxysteroid dehydrogenase-2 May Enhance Endometrial Angiogenesis via Reduced Thrombospondin-1 in Heavy Menstruation. Journal of Clinical Endocrinology and Metabolism, 94, 1443-1450. https://doi.org/10.1210/jc.2008-1879

Context: Heavy menstrual bleeding (HMB; menorrhagia) impairs quality of life for women and requires medication or surgery. Because glucocorticoids inhibit angiogenesis in other organs, we hypothesized that endometrium of women with HMB is subject to... Read More about Cortisol Inactivation by 11β-Hydroxysteroid dehydrogenase-2 May Enhance Endometrial Angiogenesis via Reduced Thrombospondin-1 in Heavy Menstruation.

Anti-inflammatory steroid signalling in the human peritoneum (2008)
Journal Article
Fegan, K. S., Rae, M. T., Critchley, H. O. D., & Hillier, S. G. (2008). Anti-inflammatory steroid signalling in the human peritoneum. Journal of Endocrinology, 196, 369-376. https://doi.org/10.1677/JOE-07-0419

Peritoneal surface epithelial (PSE) cells participate in adhesion formation following inflammatory injury yet adjacent ovarian SE (OSE) cells regenerate without scarification after ovulation. OSE cells show inflammation-associated expression of 11β h... Read More about Anti-inflammatory steroid signalling in the human peritoneum.

Ovulation and Ovarian Cancer (2008)
Book Chapter
Hillier, S. G., Rae, M. T., & Gubbay, O. (2008). Ovulation and Ovarian Cancer. In Hormonal Carcinogenesis V; (171-178). Springer. https://doi.org/10.1007/978-0-387-69080-3_16

Ovarian cancer (OC) most frequently arises from the ovarian surface epithelium (OSE), which comprises a single layer of mesothelial, squamous-to-cuboidal cells covering the entire surface of the ovary (1). This dynamic cellular layer and underlying b... Read More about Ovulation and Ovarian Cancer.

Glucocorticoid receptor-mediated regulation of MMP9 gene expression in human ovarian surface epithelial cells (2008)
Journal Article
Rae, M. T., Price, D., Harlow, C. R., Critchley, H. O. D., & Hillier, S. G. (2009). Glucocorticoid receptor-mediated regulation of MMP9 gene expression in human ovarian surface epithelial cells. Fertility and Sterility, 92(2), 703-708. https://doi.org/10.1016/j.fertnstert.2008.06.040

Objective To obtain proof-of-concept that locally produced anti-inflammatory steroids suppress ovulation-associated extracellular matrix proteases in human ovarian surface epithelial (OSE) cells. Design Primary OSE cell cultures treated with int... Read More about Glucocorticoid receptor-mediated regulation of MMP9 gene expression in human ovarian surface epithelial cells.

Thyroid hormone signaling in human ovarian surface epithelial cells. (2007)
Journal Article
Rae, M. T., Gubbay, O., NaKostogiannou, A., Price, D., Critchley, H. O. D., & Hillier, S. G. (2007). Thyroid hormone signaling in human ovarian surface epithelial cells. Journal of Clinical Endocrinology and Metabolism, 92, 322-327. doi:10.1210/jc.2006-1522

Ovarian surface epithelial (OSE) cells express multiple nuclear hormone receptor genes, including those encoding thyroid hormone and estrogen receptors (TR and ER, respectively). Ovarian cancer is hormone-dependent, and epidemiological evidence links... Read More about Thyroid hormone signaling in human ovarian surface epithelial cells..

Effects of maternal undernutrition during early pregnancy on apoptosis regulators in the ovine fetal ovary. (2006)
Journal Article
Lea, R. G., Andrade, L. P., Rae, M. T., Hannah, L. T., Kyle, C. E., Murray, J. F., …Miller, D. W. (2006). Effects of maternal undernutrition during early pregnancy on apoptosis regulators in the ovine fetal ovary. Reproduction, 131, 113-124. https://doi.org/10.1530/rep.1.00844

This study aimed to determine whether reduced fetal ovary folliculogenesis in ewes undernourished during early/midpregnancy is associated with altered ovarian cell proliferation and/or the expression of apoptosis-regulating genes. Groups of ewes (n =... Read More about Effects of maternal undernutrition during early pregnancy on apoptosis regulators in the ovine fetal ovary..

Oestrogen formation and connective tissue growth factor expression in rat granulosa cells (2006)
Journal Article
Harlow, C. R., Bradshaw, A. C., Rae, M. T., Shearer, K. D., & Hillier, S. G. (2007). Oestrogen formation and connective tissue growth factor expression in rat granulosa cells. Journal of Endocrinology, 192(1), 41-52. https://doi.org/10.1677/joe.1.06689

Ovarian follicular development involves continual remodelling of the extracellular matrix (ECM) forming the basement membrane and intercellular framework that support granulosa cell (GC) growth and differentiation. Insight into the molecular regulati... Read More about Oestrogen formation and connective tissue growth factor expression in rat granulosa cells.

Maternal nutrient restriction in early pregnancy programs hepatic mRNA expression of growth-related genes and liver size in adult male sheep (2006)
Journal Article
Hyatt, M. A., Gopalakrishnan, G. S., Bispham, J., Gentili, S., McMillen, I. C., Rhind, S. M., …Symonds, M. E. (2007). Maternal nutrient restriction in early pregnancy programs hepatic mRNA expression of growth-related genes and liver size in adult male sheep. Journal of Endocrinology, 192(1), 87-97. https://doi.org/10.1677/joe.1.06801

The liver is a major metabolic and endocrine organ of critical importance in the regulation of growth and metabolism. Its function is determined by a complex interaction of nutritionally regulated counter-regulatory hormones. The extent to which hepa... Read More about Maternal nutrient restriction in early pregnancy programs hepatic mRNA expression of growth-related genes and liver size in adult male sheep.

cAMP response element-binding (CREB) signalling and ovarian surface epithelial cell survival (2006)
Journal Article
Gubbay, O., Rae, M. T., McNeilly, A. S., Donadeu, F. X., Zeleznik, A. J., & Hillier, S. G. (2006). cAMP response element-binding (CREB) signalling and ovarian surface epithelial cell survival. Journal of Endocrinology, 191(1), 275-285. https://doi.org/10.1677/joe.1.06928

cAMP response-element binding (CREB) transcription factors transduce cell survival responses to peptide hormones and growth factors in normal tissues and mutant CREB proteins are implicated in tumorigenesis. Ovarian cancer most frequently arises from... Read More about cAMP response element-binding (CREB) signalling and ovarian surface epithelial cell survival.

Steroid signalling in the ovarian surface epithelium (2005)
Journal Article
Rae, M. T., & Hillier, S. G. (2005). Steroid signalling in the ovarian surface epithelium. Trends in Endocrinology and Metabolism, 16(7), 327-333. https://doi.org/10.1016/j.tem.2005.07.002

Human ovarian surface epithelium (HOSE) undergoes serial injury-repair with each ovulation, which is probably why most ovarian epithelial cancers arise there. Considering the proposed inflammatory aetiology of ovarian cancer, anti-inflammatory steroi... Read More about Steroid signalling in the ovarian surface epithelium.

Inflammation-associated gene expression is altered between normal human ovarian surface epithelial cells and cell lines derived from ovarian adenocarcinomas (2005)
Journal Article
Gubbay, O., Guo, W., Rae, M. T., Niven, D., Langdon, S. P., & Hillier, S. G. (2005). Inflammation-associated gene expression is altered between normal human ovarian surface epithelial cells and cell lines derived from ovarian adenocarcinomas. British Journal of Cancer, 92, 1927-1933. https://doi.org/10.1038/sj.bjc.6602568

Ovulation is believed to contribute to the development of ovarian cancers that derive from the ovarian surface epithelium (OSE). The process of ovulation is synonymous with inflammation and inflammatory cytokines such as interleukin-1α (IL-1α) have r... Read More about Inflammation-associated gene expression is altered between normal human ovarian surface epithelial cells and cell lines derived from ovarian adenocarcinomas.

Impact of maternal undernutrition and fetal number on glucocorticoid, growth hormone and insulin-like growth factor receptor mRNA abundance in the ovine fetal kidney (2005)
Journal Article
Brennan, K. A., Gopalakrishnan, G. S., Kurlak, L., Rhind, S. M., Kyle, C. E., Brooks, A. N., …Symonds, M. E. (2005). Impact of maternal undernutrition and fetal number on glucocorticoid, growth hormone and insulin-like growth factor receptor mRNA abundance in the ovine fetal kidney. Reproduction, 129, 151-159. https://doi.org/10.1530/rep.1.00229

Epidemiological and animal studies strongly indicate that the environment experienced in utero determines, in part, an individual’s likelihood of developing cardiovascular disease in later life. This risk has been further linked to impaired kidney fu... Read More about Impact of maternal undernutrition and fetal number on glucocorticoid, growth hormone and insulin-like growth factor receptor mRNA abundance in the ovine fetal kidney.

Anti-inflammatory and proliferative responses in human and ovine ovarian surface epithelial cells (2004)
Journal Article
Gubbay, O., Guo, W., Rae, M. T., Niven, D., Howie, A. F., McNeilly, A. S., …Hillier, S. G. (2004). Anti-inflammatory and proliferative responses in human and ovine ovarian surface epithelial cells. Reproduction, 128, 607-614. https://doi.org/10.1530/rep.1.00272

The majority of ovarian cancers (>90%) are believed to derive from the ovarian surface epithelium (OSE); a single layer covering the entire surface of the ovary. At ovulation, the OSE cell layer undergoes an inflammatory response, involving cell deat... Read More about Anti-inflammatory and proliferative responses in human and ovine ovarian surface epithelial cells.

Steroid signalling in human ovarian surface epithelial cells: the response to interleukin-1alpha determined by microarray analysis. (2004)
Journal Article
Rae, M. T., Niven, D., Ross, A., Forster, T., Lathe, R., Critchley, H. O. D., …Hillier, S. G. (2004). Steroid signalling in human ovarian surface epithelial cells: the response to interleukin-1alpha determined by microarray analysis. Journal of Endocrinology, 183, 19-28. https://doi.org/10.1677/joe.1.05754

The human ovarian surface epithelium (HOSE) is a common site of gynaecological disease including endometriosis and ovarian cancer, probably due to serial injury-repair events associated with successive ovulations. To comprehend the importance of ster... Read More about Steroid signalling in human ovarian surface epithelial cells: the response to interleukin-1alpha determined by microarray analysis..

Antiinflammatory Steroid Action in Human Ovarian Surface Epithelial Cells (2004)
Journal Article
Hillier, S. G., Rae, M. T., Niven, D., Critchley, H. O. D., & Harlow, C. R. (2004). Antiinflammatory Steroid Action in Human Ovarian Surface Epithelial Cells. Journal of Clinical Endocrinology and Metabolism, 89(9), 4538-4544. https://doi.org/10.1210/jc.2003-032225

The human ovarian surface epithelium (OSE) is subject to serial injury and repair during ovulation, which is a natural inflammatory event. We asked whether there is a compensatory antiinflammatory component to this process, involving steroid hormone... Read More about Antiinflammatory Steroid Action in Human Ovarian Surface Epithelial Cells.

Programming of adult cardiovascular function after early maternal undernutrition in sheep (2004)
Journal Article
Gopalakrishnan, G. S., Gardner, D. S., Rhind, S. M., Rae, M. T., Kyle, C. E., Brooks, A. N., …Symonds, M. E. (2004). Programming of adult cardiovascular function after early maternal undernutrition in sheep. American journal of physiology: regulatory, integrative and comparative physiology, 287(1), R12-R20. https://doi.org/10.1152/ajpregu.00687.2003

The prenatal nutritional environment influences the subsequent risk of hypertension in adulthood. Animal studies have used, generally, the rat as a model species to illustrate the association between maternal nutrient intake and blood pressure in the... Read More about Programming of adult cardiovascular function after early maternal undernutrition in sheep.

Effects of prenatal undernutrition on emotional reactivity and cognitive flexibility in adult sheep (2003)
Journal Article
Erhard, H. W., Boissy, A., Rae, M. T., & Rhind, S. M. (2004). Effects of prenatal undernutrition on emotional reactivity and cognitive flexibility in adult sheep. Behavioural Brain Research, 151(1-2), 25-35. https://doi.org/10.1016/j.bbr.2003.08.003

The experiment reported was designed to test two hypotheses: that prenatal undernutrition (a) increases emotional reactivity and (b) impairs cognitive flexibility in sheep. The mothers of one group were fed live weight maintenance requirements throug... Read More about Effects of prenatal undernutrition on emotional reactivity and cognitive flexibility in adult sheep.

Glucocorticoid metabolism and reproduction: a tale of two enzymes (2003)
Journal Article
Michael, A., Thurston, L., & Rae, M. (2003). Glucocorticoid metabolism and reproduction: a tale of two enzymes. Reproduction, 126, 425-441. https://doi.org/10.1530/rep.0.1260425

Within potential target cells, the actions of physiological glucocorticoids (cortisol and corticosterone) are modulated by isoforms of the enzyme 11 beta-hydroxysteroid dehydrogenase (11 beta HSD). To date, two isoforms of 11 beta HSD have been clone... Read More about Glucocorticoid metabolism and reproduction: a tale of two enzymes.

Environmental influences on the fetus and neonate—timing, mechanisms of action and effects on subsequent adult function (2003)
Journal Article
Rhind, S., Rae, M., & Brooks, A. (2003). Environmental influences on the fetus and neonate—timing, mechanisms of action and effects on subsequent adult function. Domestic Animal Endocrinology, 25(1), 3-11. https://doi.org/10.1016/S0739-7240%2803%2900041-9

Environmental influences on fetal and neonatal development can affect neural, reproductive, immune and cardiovascular function in adult humans and animals. The effects can be exerted at many different stages of development from before conception to a... Read More about Environmental influences on the fetus and neonate—timing, mechanisms of action and effects on subsequent adult function.

Undernutrition of ewe lambs in utero and in early post-natal life does not affect hypothalamic–pituitary function in adulthood (2003)
Journal Article
Borwick, S., Rae, M., Brooks, J., McNeilly, A., Racey, P., & Rhind, S. (2003). Undernutrition of ewe lambs in utero and in early post-natal life does not affect hypothalamic–pituitary function in adulthood. Animal Reproduction Science, 77(1-2), 61-70. https://doi.org/10.1016/S0378-4320%2802%2900261-0

The effect of undernutrition in utero, during late gestation (from day 100), and early neonatal life on hypothalamic–pituitary function was investigated in female lambs born to ewes fed rations calculated to provide either 100% (high; H) or 70% (low;... Read More about Undernutrition of ewe lambs in utero and in early post-natal life does not affect hypothalamic–pituitary function in adulthood.

Lysyl Oxidase Gene Expression and Enzyme Activity in the Rat Ovary: Regulation by Follicle-Stimulating Hormone, Androgen, and Transforming Growth Factor-β Superfamily Members in Vitro (2003)
Journal Article
Harlow, C. R., Rae, M., Davidson, L., Trackman, P. C., & Hillier, S. G. (2003). Lysyl Oxidase Gene Expression and Enzyme Activity in the Rat Ovary: Regulation by Follicle-Stimulating Hormone, Androgen, and Transforming Growth Factor-β Superfamily Members in Vitro. Endocrinology, 144(1), 154-162. https://doi.org/10.1210/en.2002-220652

Lysyl oxidase (LOX) catalyzes the final enzymatic reaction required for cross-linking of collagen and elastin fibers and therefore has a crucial role in regulating the formation and maintenance of extracellular matrix in the ovary. LOX mRNA is abunda... Read More about Lysyl Oxidase Gene Expression and Enzyme Activity in the Rat Ovary: Regulation by Follicle-Stimulating Hormone, Androgen, and Transforming Growth Factor-β Superfamily Members in Vitro.

Effect of maternal undernutrition on fetal testicular steroidogenesis during the CNS androgen-responsive period in male sheep fetuses (2002)
Journal Article
Rae, M., Rhind, S., Fowler, P., Miller, D. W., Kyle, C., & Brooks, A. (2002). Effect of maternal undernutrition on fetal testicular steroidogenesis during the CNS androgen-responsive period in male sheep fetuses. Reproduction, 124, 33-39. https://doi.org/10.1530/rep.0.1240033

The aim of this study was to determine the effects of maternal undernutrition, applied during physiologically relevant stages of development of the reproductive system, on reproductive development in male sheep fetuses. Groups of ewes (n = 11-19) wer... Read More about Effect of maternal undernutrition on fetal testicular steroidogenesis during the CNS androgen-responsive period in male sheep fetuses.

Non-genomic steroid receptors in the bovine ovary (2002)
Journal Article
Bramley, T., Menzies, G., Rae, M., & Scobie, G. (2002). Non-genomic steroid receptors in the bovine ovary. Domestic Animal Endocrinology, 23(1-2), 3-12. https://doi.org/10.1016/S0739-7240%2802%2900140-6

Over the last few years, rapid and physiologically important non-genomic actions of all classes of steroid hormones have been described in many cell types. A putative non-genomic membrane progesterone receptor (NGPR) was the first, and so far the onl... Read More about Non-genomic steroid receptors in the bovine ovary.

Maternal undernutrition alters triiodothyronine concentrations and pituitary response to GnRH in fetal sheep (2002)
Journal Article
Rae, M., Rhind, S., Kyle, C., Miller, D. W., & Brooks, A. (2002). Maternal undernutrition alters triiodothyronine concentrations and pituitary response to GnRH in fetal sheep. Journal of Endocrinology, 173, 449-455. https://doi.org/10.1677/joe.0.1730449

The aims of this study were to determine which hormones may have a role in the expression of maternal undernutrition effects on reproductive function, in both the developing fetus and the adult offspring. This was undertaken by measuring the effects... Read More about Maternal undernutrition alters triiodothyronine concentrations and pituitary response to GnRH in fetal sheep.

The effects of undernutrition, in utero, on reproductive function in adult male and female sheep (2002)
Journal Article
Rae, M., Kyle, C., Miller, D., Hammond, A., Brooks, A., & Rhind, S. (2002). The effects of undernutrition, in utero, on reproductive function in adult male and female sheep. Animal Reproduction Science, 72(1-2), 63-71. https://doi.org/10.1016/S0378-4320%2802%2900068-4

The aim of this study was to determine the effects of maternal undernutrition during pregnancy on adult reproductive function in male and female offspring. Groups of ewes were fed rations providing either 100% (High, H) or 50% (Low, L) of estimated m... Read More about The effects of undernutrition, in utero, on reproductive function in adult male and female sheep.

Effect of maternal undernutrition during pregnancy on early ovarian development and subsequent follicular development in sheep fetuses (2001)
Journal Article
Rae, M., Palassio, S., Kyle, C., Brooks, A., Lea, R., Miller, D., & Rhind, S. (2001). Effect of maternal undernutrition during pregnancy on early ovarian development and subsequent follicular development in sheep fetuses. Reproduction, 122, 915-922. https://doi.org/10.1530/rep.0.1220915

Gonad development in female sheep fetuses is thought to occur in a number of key stages. The aim of this study was to determine the effects of maternal undernutrition, applied at one or more of these critical stages, on fetal ovarian development. Gro... Read More about Effect of maternal undernutrition during pregnancy on early ovarian development and subsequent follicular development in sheep fetuses.

Synthesis and Characterization of 111In−DTPA−N-TIMP-2: A Radiopharmaceutical for Imaging Matrix Metalloproteinase Expression (2001)
Journal Article
Giersing, B. K., Rae, M. T., CarballidoBrea, M., Williamson, R. A., & Blower, P. J. (2001). Synthesis and Characterization of 111In−DTPA−N-TIMP-2: A Radiopharmaceutical for Imaging Matrix Metalloproteinase Expression. Bioconjugate Chemistry, 12, 964-971. https://doi.org/10.1021/bc010028f

The matrix metalloproteinases (MMPs) are enzymes involved in the turnover of the extracellular matrix. Their overexpression in tumors is implicated in the metastatic process and may provide a target for diagnostic tumor imaging by using a radiolabel... Read More about Synthesis and Characterization of 111In−DTPA−N-TIMP-2: A Radiopharmaceutical for Imaging Matrix Metalloproteinase Expression.

Effects of nutrition and environmental factors on the fetal programming of the reproductive axis (2001)
Journal Article
Rhind, S., Rae, M., & Brooks, A. (2001). Effects of nutrition and environmental factors on the fetal programming of the reproductive axis. Reproduction, 122, 205-214. https://doi.org/10.1530/rep.0.1220205

Research from a wide range of scientific disciplines has shown that the reproductive performance of animals in adult life is determined, in part, by a variety of extraneous influences acting at different stages of development from before conception u... Read More about Effects of nutrition and environmental factors on the fetal programming of the reproductive axis.

Stimulation of specific binding of [3H]-progesterone to bovine luteal cell-surface membranes: specificity of digitonin (1999)
Journal Article
Menzies, G., Howland, K., Rae, M., & Bramley, T. (1999). Stimulation of specific binding of [3H]-progesterone to bovine luteal cell-surface membranes: specificity of digitonin. Molecular and Cellular Endocrinology, 153, 57-69. https://doi.org/10.1016/S0303-7207%2899%2900091-X

Non-genomic actions of progesterone have been described in the ovary, and luteal membranes of several species have been shown to possess specific binding sites for [3H]-progesterone. However, binding of radiolabelled progesterone to luteal membranes... Read More about Stimulation of specific binding of [3H]-progesterone to bovine luteal cell-surface membranes: specificity of digitonin.

Bovine ovarian non-genomic progesterone binding sites: presence in follicular and luteal cell membranes (1998)
Journal Article
Rae, M., Menzies, G., & Bramley, T. (1998). Bovine ovarian non-genomic progesterone binding sites: presence in follicular and luteal cell membranes. Journal of Endocrinology, 159, 413-427. https://doi.org/10.1677/joe.0.1590413

We have shown recently that the bovine corpus luteum (CL) possesses specific luteal cell surface membrane binding sites for progesterone. We have now confirmed and extended these observations to compare the subcellular distribution of these binding s... Read More about Bovine ovarian non-genomic progesterone binding sites: presence in follicular and luteal cell membranes.

Design of hypoxia-targeting radiopharmaceuticals: selective uptake of copper-64 complexes in hypoxic cells in vitro (1998)
Journal Article
Dearling, J. L. J., Lewis, J. S., Mullen, G. E. D., Rae, M. T., Zweit, J., & Blower, P. J. (1998). Design of hypoxia-targeting radiopharmaceuticals: selective uptake of copper-64 complexes in hypoxic cells in vitro. European Journal of Nuclear Medicine and Molecular Imaging, 25(7), 788-792. https://doi.org/10.1007/s002590050283

The well-known perfusion tracer CuPTSM, labelled with 62Cu or 64Cu, is believed to be trapped in cells non-selectively by a bioreductive mechanism. It is proposed that by modifying the ligand to increase its electron donor strength (for example by ad... Read More about Design of hypoxia-targeting radiopharmaceuticals: selective uptake of copper-64 complexes in hypoxic cells in vitro.

Specific Non-Genomic, Membrane-Localized Binding Sites for Progesterone in the Bovine Corpus Luteum1 (1998)
Journal Article
Rae, M. T., Menzies, G. S., McNeilly, A. S., Woad, K., Webb, R., & Bramley, T. A. (1998). Specific Non-Genomic, Membrane-Localized Binding Sites for Progesterone in the Bovine Corpus Luteum1. Biology of Reproduction, 58(6), 1394-1406. https://doi.org/10.1095/biolreprod58.6.1394

Fractionation of bovine corpus luteum (CL) homogenates on continuous sucrose density gradients with and without preincubation with 3H-progesterone demonstrated high levels of tracer binding and high content of endogenous progesterone associated with... Read More about Specific Non-Genomic, Membrane-Localized Binding Sites for Progesterone in the Bovine Corpus Luteum1.

Protein kinase C- and Ca2+ ionophore-stimulated production of reactive oxygen species in mechanically dispersed isolated bovine luteal cells (1997)
Journal Article
Sakka, E., Rae, M., Bramley, T., & Aitken, J. (1997). Protein kinase C- and Ca2+ ionophore-stimulated production of reactive oxygen species in mechanically dispersed isolated bovine luteal cells. Biology of Reproduction, 57(2), 428-435

We measured the production of reactive oxygen species (ROS) using luminol-horseradish peroxidase-induced chemiluminescence in mechanically dispersed cell suspensions from bovine corpus luteum (CL). Since other cell types besides luteal cells were pre... Read More about Protein kinase C- and Ca2+ ionophore-stimulated production of reactive oxygen species in mechanically dispersed isolated bovine luteal cells.