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Development of an acute ovine model of polycystic ovaries to assess the effect of ovarian denervation

Duncan, W. Colin; Nicol, Linda M.; O’Hare, Rosie; Witherington, Jason; Miranda, Jason A.; Campbell, Bruce K.; Thomas, Jennifer L.; Rae, Michael T.


W. Colin Duncan

Linda M. Nicol

Rosie O’Hare

Jason Witherington

Jason A. Miranda

Bruce K. Campbell

Jennifer L. Thomas


Introduction: Polycystic ovary syndrome (PCOS) seems to be associated with increased ovarian sympathetic nerve activity and in rodent models of PCOS reducing the sympathetic drive to the ovary, through denervation or neuromodulation, improves ovulation rate. We hypothesised that sympathetic nerves work with gonadotropins to promote development and survival of small antral follicles to develop a polycystic ovary phenotype.

Methods: Using a clinically realistic ovine model we showed a rich sympathetic innervation to the normal ovary and reinnervation after ovarian transplantation. Using needlepoint diathermy to the nerve plexus in the ovarian vascular pedicle we were able to denervate the ovary resulting in reduced intraovarian noradrenaline and tyrosine hydroxylase immunostained sympathetic nerves. We developed an acute polycystic ovary (PCO) model using gonadotrophin releasing hormone (GnRH) agonist followed infusion of follicle stimulating hormone (FSH) with increased pulsatile luteinising hormone (LH). This resulted in increased numbers of smaller antral follicles in the ovary when compared to FSH infusion suggesting a polycystic ovary.

Results: Denervation had no effect of the survival or numbers of follicles in the acute PCO model and did not impact on ovulation, follicular and luteal hormone profiles in a normal cycle.

Discussion: Although the ovary is richly inervated we did not find evidence for a role of sympathetic nerves in ovarian function or small follicle growth and survival

Journal Article Type Article
Acceptance Date Oct 10, 2023
Online Publication Date Oct 24, 2023
Publication Date 2023
Deposit Date Oct 30, 2023
Publicly Available Date Oct 30, 2023
Journal Frontiers in Endocrinology
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 14
Article Number 1285269
Keywords follicle, tyrosine hydroxylase, polycystic ovary syndrome, sympathetic nerve, gonadotrophin
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