All Outputs (41)

Characterisation of autophagy induction by the thiopurine drugs azathioprine, mercaptopurine and thioguanine in THP-1 macrophages (2024)
Journal Article
Masson, C. D., Findlay-Greene, F., Henderson Sousa, F., Henderson, P., Fraser, J. A., Barlow, P. G., & Stevens, C. (online). Characterisation of autophagy induction by the thiopurine drugs azathioprine, mercaptopurine and thioguanine in THP-1 macrophages. Naunyn-Schmiedeberg's Archives of Pharmacology, https://doi.org/10.1007/s00210-024-03563-0

Purpose: Activating autophagy may be therapeutically beneficial, and we have previously shown that azathioprine (AZA), an immunomodulatory drug, induces autophagy. Here, we evaluated the induction of autophagy by the thiopurines AZA, mercaptopurine (... Read More about Characterisation of autophagy induction by the thiopurine drugs azathioprine, mercaptopurine and thioguanine in THP-1 macrophages.

Evolution and immunopathology of chikungunya virus informs therapeutic development (2023)
Journal Article
Henderson Sousa, F., Ghaisani Komarudin, A., Findlay-Greene, F., Bowolaksono, A., Sasmono, R. T., Stevens, C., & Barlow, P. G. (2023). Evolution and immunopathology of chikungunya virus informs therapeutic development. Disease Models and Mechanisms, 16(4), https://doi.org/10.1242/dmm.049804

Chikungunya virus (CHIKV), a mosquito-borne alphavirus, is an emerging global threat identified in more than 60 countries across continents. The risk of CHIKV transmission is rising due to increased global interactions, year-round presence of mosquit... Read More about Evolution and immunopathology of chikungunya virus informs therapeutic development.

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) (2021)
Journal Article
Klionsky, D. J., Kamal Abdel-Aziz, A., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Stevens, C., & et al. (2021). Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy, 17(1), 1-382. https://doi.org/10.1080/15548627.2020.1797280

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have als... Read More about Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition).

Models of osteoarthritis: relevance and new insights (2020)
Journal Article
Samvelyan, H., Hughes, D., Stevens, C., & Staines, K. A. (2021). Models of osteoarthritis: relevance and new insights. Calcified Tissue International, 109(3), 243-256. https://doi.org/10.1007/s00223-020-00670-x

Osteoarthritis (OA) is a progressive and disabling musculoskeletal disease affecting millions of people and resulting in major healthcare costs worldwide. It is the most common form of arthritis, characterised by degradation of the articular cartilag... Read More about Models of osteoarthritis: relevance and new insights.

Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection (2020)
Journal Article
Casanova, V., Sousa, F. H., Shakamuri, P., Svoboda, P., Buch, C., D'Acremont, M., Christophorou, M. A., Pohl, J., Stevens, C., & Barlow, P. G. (2020). Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection. Frontiers in Immunology, 11, Article 85. https://doi.org/10.3389/fimmu.2020.00085

Human rhinoviruses (HRV) are the most common cause of viral respiratory tract infections. While normally mild and self-limiting in healthy adults, HRV infections are associated with bronchiolitis in infants, pneumonia in immunocompromised patients, a... Read More about Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection.

Design of a New Peptide Substrate Probe of the Putative Biomarker Legumain with Potential Application in Prostate Cancer Diagnosis ex vivo (2019)
Journal Article
Mathur, S., Turnbull, A., Akaev, I., Stevens, C., Agrawal, N., Chopra, M., & Mincher, D. (2020). Design of a New Peptide Substrate Probe of the Putative Biomarker Legumain with Potential Application in Prostate Cancer Diagnosis ex vivo. International Journal of Peptide Research and Therapeutics, 26, 1965-1980. https://doi.org/10.1007/s10989-019-09994-1

The lysosomal endoprotease legumain (asparaginyl endoprotease) has been proposed as a putative biomarker in prostate tumours, in which the enzyme is markedly overexpressed. Overexpression, coupled with highly selective specificity for cleavage of sub... Read More about Design of a New Peptide Substrate Probe of the Putative Biomarker Legumain with Potential Application in Prostate Cancer Diagnosis ex vivo.

Azathioprine Has a Deleterious Effect on the Bone Health of Mice with DSS-Induced Inflammatory Bowel Disease (2019)
Journal Article
Morgan, S., Hooper, K. M., Milne, E. M., Farquharson, C., Stevens, C., & Staines, K. A. (2019). Azathioprine Has a Deleterious Effect on the Bone Health of Mice with DSS-Induced Inflammatory Bowel Disease. International Journal of Molecular Sciences, 20(23), Article 6085. https://doi.org/10.3390/ijms20236085

Patients with inflammatory bowel disease (IBD) often present poor bone health and are 40% more at risk of bone fracture. Studies have implicated autophagy in IBD pathology and drugs used to treat IBD stimulate autophagy in varying degrees, however, t... Read More about Azathioprine Has a Deleterious Effect on the Bone Health of Mice with DSS-Induced Inflammatory Bowel Disease.

Regulation of the expression of DAPK1 by SUMO pathway. (2019)
Journal Article
Wang, Q., Zhang, X., Chen, L., Weng, S., Xia, Y., Ye, Y., …Lin, Y. (2019). Regulation of the expression of DAPK1 by SUMO pathway. Biomolecules, 9(4), 151. https://doi.org/10.3390/biom9040151

Death Associated Protein Kinase 1 (DAPK1) is an important signaling kinase mediating the biological eect of multiple natural biomolecules such as IFN-, TNF-, curcumin, etc. DAPK1 is degraded through both ubiquitin-proteasomal and lysosomal degradat... Read More about Regulation of the expression of DAPK1 by SUMO pathway..

The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK (2019)
Journal Article
Hooper, K. M., Casanova, V., Kemp, S., Staines, K. A., Satsangi, J., Barlow, P. G., Henderson, P., & Stevens, C. (2019). The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK. Inflammatory Bowel Diseases, 25(9), 1481-1496. https://doi.org/10.1093/ibd/izz039

Background Genetic studies have strongly linked autophagy to Crohn's disease (CD) and stimulating autophagy in CD patients may be therapeutically beneficial. The aim of this study was to evaluate the effect of current inflammatory bowel disease (IBD)... Read More about The inflammatory bowel disease drug azathioprine induces autophagy via mTORC1 and the unfolded protein response sensor PERK.

The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy (2019)
Journal Article
Biskou, O., Casanova, V., Hooper, K., Kemp, S., Wright, G. P., Satsangi, J., Barlow, P., & Stevens, C. (2019). The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy. PLOS ONE, 14(1), Article e0209665. https://doi.org/10.1371/journal.pone.0209665

The cytoskeletal protein vimentin plays a key role in positioning of organelles within the cytosol and has been linked to the regulation of numerous cellular processes including autophagy, however, how vimentin regulates autophagy remains relatively... Read More about The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy.

Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease (2018)
Journal Article
Hooper, K. M., Barlow, P. G., Henderson, P., & Stevens, C. (2019). Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease. Inflammatory Bowel Diseases, 25(4), 661-671. https://doi.org/10.1093/ibd/izy380

Inflammatory Bowel Disease (IBD), which includes Crohn's disease (CD) and ulcerative colitis, is characterised by chronic inflammation of the gastrointestinal tract. Aetiology involves a combination of genetic and environmental factors resulting in a... Read More about Interactions Between Autophagy and the Unfolded Protein Response: Implications for Inflammatory Bowel Disease.

Antiviral therapeutic approaches for human rhinovirus infections (2018)
Journal Article
Casanova, V., Sousa, F. H., Stevens, C., & Barlow, P. G. (2018). Antiviral therapeutic approaches for human rhinovirus infections. Future Virology, https://doi.org/10.2217/fvl-2018-0016

Human rhinoviruses (RV) are the primary etiological agent of the common cold. This infection can be mild and self-limiting in immunocompetent hosts, but can be associated with bronchiolitis in infants, pneumonia in the immunosuppressed, and exacerba... Read More about Antiviral therapeutic approaches for human rhinovirus infections.

Cathelicidins display conserved direct antiviral activity towards rhinovirus. (2017)
Journal Article
Sousa, F. H., Casanova, V., Findlay, F., Stevens, C., Svoboda, P., Pohl, J., Proudfoot, L., & Barlow, P. G. (2017). Cathelicidins display conserved direct antiviral activity towards rhinovirus. Peptides, 95, 76-83. https://doi.org/10.1016/j.peptides.2017.07.013

Human rhinoviruses (HRVs) are the most common cause of viral respiratory tract infections, and are associated with significant morbidity and mortality in immunocompromised individuals and patients with pre-existing pulmonary conditions. The therapeut... Read More about Cathelicidins display conserved direct antiviral activity towards rhinovirus..

Design and evaluation of novel theranostic fluorogenic dual probe-prodrug in cancer (2016)
Presentation / Conference Contribution
Mathur, S., Mincher, D., Turnbull, A., Stevens, C., & Poole, A. (2016). Design and evaluation of novel theranostic fluorogenic dual probe-prodrug in cancer. European Journal of Cancer, 61, S142. https://doi.org/10.1016/s0959-8049%2816%2961501-0

Background: In spite of major advances in the diagnosis and treatment of cancer, there remains a paucity of biomarkers for early detection. Legumain is a potential cancer biomarker and a molecular target for imaging and drug targeting. Legumain is a... Read More about Design and evaluation of novel theranostic fluorogenic dual probe-prodrug in cancer.

Inflammatory Bowel Disease Drugs: A Focus on Autophagy (2016)
Journal Article
Hooper, K. M., Barlow, P. G., Stevens, C., & Henderson, P. (2017). Inflammatory Bowel Disease Drugs: A Focus on Autophagy. Journal of Crohn's and Colitis, 11(1), 118-127. https://doi.org/10.1093/ecco-jcc/jjw127

Inflammatory bowel disease [IBD] is characterized by chronic inflammation of the gastrointestinal tract. Medications such as corticosteroids, thiopurines, immunomodulators and biologic agents are used to induce and maintain remission; however, respon... Read More about Inflammatory Bowel Disease Drugs: A Focus on Autophagy.

Cationic host defense peptides; novel antimicrobial therapeutics against Category A pathogens and emerging infections (2016)
Journal Article
Findlay, F., Proudfoot, L., Stevens, C., & Barlow, P. G. (2016). Cationic host defense peptides; novel antimicrobial therapeutics against Category A pathogens and emerging infections. Pathogens and Global Health, 110(4-5), 137-147. https://doi.org/10.1080/20477724.2016.1195036

Cationic Host Defense Peptides (HDP, also known as antimicrobial peptides) are crucial components of the innate immune system and possess broad-spectrum antibacterial, antiviral, and immunomodulatory activities. They can contribute to the rapid clear... Read More about Cationic host defense peptides; novel antimicrobial therapeutics against Category A pathogens and emerging infections.

Antiviral host defence peptides. (2016)
Book Chapter
Sousa, F. H., Casanova, V., Stevens, C., & Barlow, P. G. (2016). Antiviral host defence peptides. In R. M. Epand (Ed.), Host Defense Peptides and Their Potential as Therapeutic Agents (57-94). Springer. https://doi.org/10.1007/978-3-319-32949-9_3

The on going global mortality and morbidity associated with viral pathogens highlights the need for the continued development of effective, novel antiviral molecules. The antiviral activity of cationic host defence peptides is of significant interest... Read More about Antiviral host defence peptides..

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). (2016)
Journal Article
Baghdiguian, S., Bagniewska-Zadworna, A., Baek, S., Baehrecke, E. H., Bae, S. H., Bae, D., Bae, J., Backer, J. M., Azad, N., Bachetti, T., Awale, S., Avantaggiati, M. L., Avrahami, L., Avagliano, L., Autelli, R., Auburger, G., Aurelian, L., Auberger, P., Attardi, L. D., Atherton, S. S., …Stevens, C. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12(1), 1-222. https://doi.org/10.1080/15548627.2015.1100356

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies... Read More about Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)..

PWE-090 the effect of commonly used Ibd drugs on autophagy induction using an in vitro cell culture system. (2014)
Journal Article
Henderson, P., Satsangi, J., Wilson, D., & Stevens, C. (2014). PWE-090 the effect of commonly used Ibd drugs on autophagy induction using an in vitro cell culture system. Gut, 63(Suppl 1), A163. https://doi.org/10.1136/gutjnl-2014-307263.350

Introduction Genome wide association studies and functional experiments in inflammatory bowel disease (IBD) have delineated the importance of autophagy in IBD pathogenesis. We aimed to determine the effect of commonly utilised IBD drugs on autophagy... Read More about PWE-090 the effect of commonly used Ibd drugs on autophagy induction using an in vitro cell culture system..

Autophagic targeting of Src promotes cancer cell survival following reduced FAK signalling (2012)
Journal Article
Sandilands, E., Serrels, B., McEwan, D. G., Morton, J. P., Macagno, J. P., McLeod, K., Stevens, C., Brunton, V. G., Langdon, W. Y., Vidal, M., Sansom, O. J., Dikic, I., Wilkinson, S., & Frame, M. C. (2012). Autophagic targeting of Src promotes cancer cell survival following reduced FAK signalling. Nature Cell Biology, 14(1), 51-60. https://doi.org/10.1038/ncb2386

Here we describe a mechanism that cancer cells use to survive when flux through the Src/FAK pathway is severely perturbed. Depletion of FAK, detachment of FAK-proficient cells or expression of non-phosphorylatable FAK proteins causes sequestration of... Read More about Autophagic targeting of Src promotes cancer cell survival following reduced FAK signalling.