Skip to main content

Research Repository

Advanced Search

Inflammatory tendencies and overproduction of IL-17 in the colon of young NOD mice are counteracted with diet change

Alam, Catharina; Valkonen, Suvi; Palagani, Vindhya; Jalava, Jari; Eerola, Erkki; H�nninen, Arno


Suvi Valkonen

Vindhya Palagani

Jari Jalava

Erkki Eerola

Arno H�nninen


Dietary factors influence diabetes development in the NOD mouse. Diet affects the composition of microbiota in the distal intestine, which may subsequently influence intestinal immune homeostasis. However, the specific effects of antidiabetogenic diets on gut immunity and the explicit associations between intestinal immune disruption and type 1 diabetes onset remain unclear.
Research design and methods
Gut microbiota of NOD mice fed a conventional diet or ProSobee formula were compared using gas chromatography. Colonic lamina propria immune cells were characterized in terms of activation markers, cytokine mRNA and Th17 and Foxp3(+) T-cell numbers, using real-time PCR and flow cytometry. Activation of diabetogenic CD4 T-cells by purified B-cells was assessed in both groups. Immune tolerance to autologous commensal bacteria was evaluated in vitro using thymidine-incorporation tests.
Young NOD mice showed a disturbed tolerance to autologous commensal bacteria. Increased numbers of activated CD4 T-cells and (CD11b(+)CD11c(+)) dendritic cells and elevated levels of Th17 cells and IL23 mRNA were moreover observed in colon lamina propria. These phenomena were abolished when mice were fed an antidiabetogenic diet. The antidiabetogenic diet also altered the expression levels of costimulatory molecules and the capacity of peritoneal B-cells to induce insulin-specific CD4 T-cell proliferation.
Young NOD mice show signs of subclinical colitis, but the symptoms are alleviated by a diet change to an antidiabetogenic diet. Disrupted immune tolerance in the distal intestine may influence peritoneal cell pools and B-cell-mediated activation of diabetogenic T-cells.

Journal Article Type Article
Acceptance Date May 25, 2010
Online Publication Date Jun 14, 2010
Publication Date 2010-09
Deposit Date May 26, 2022
Publicly Available Date Jun 28, 2023
Journal Diabetes
Print ISSN 0012-1797
Publisher American Diabetes Association
Peer Reviewed Peer Reviewed
Volume 59
Issue 9
Pages 2237—2246
Public URL
Related Public URLs


You might also like

Downloadable Citations