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Diatom-inspired skeletonisation of insulin - Mechanistic insights into crystallisation and extracellular bioactivity

V�liz, Dios�ngeles Soto; Alam, Catharina; Nietzel, Thiago; Wyborski, Rebecca; Rivero-M�ller, Adolfo; Alam, Parvez


Dios�ngeles Soto V�liz

Thiago Nietzel

Rebecca Wyborski

Adolfo Rivero-M�ller

Parvez Alam


In this paper, we encage insulin within calcium carbonate by means of a biomineralisation process. We find that both dogbone and crossbone morphologies develop during the crystallisation process. The crystals break down into small nanocrystals after prolonged immersion in phosphate buffer solution, which adhere extracellularly to mammalian cells without causing any observable damage or early cell-death. The mechanisms behind calcium carbonate encaging of single insulin monomers are detailed. This communication elucidates a novel, diatom-inspired approach to the mineral skeletonisation of insulin.

Journal Article Type Article
Acceptance Date May 31, 2015
Online Publication Date Jun 9, 2015
Publication Date 2015-09
Deposit Date May 26, 2022
Journal Colloids and surfaces. B, Biointerfaces
Print ISSN 0927-7765
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 133
Pages 140-147
Keywords Calcium carbonate, Crystallisation, Direct encapsulation, Insulin, Diatom, Diabetes
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