Apoptosis does not contribute to the blood lymphocytopenia observed after intensive and downhill treadmill running in humans.

Simpson, Richard J; Florida-James, Geraint; Whyte, Greg P; Black, James; Ross, James; Guy, Keith

Abstract

The lymphocytopenia that occurs during the recovery stage of exercise may be a result of apoptosis through an increased expression of CD95, a loss of the complement regulatory proteins CD55 and CD59, or both. Trained subjects completed intensive, moderate, and downhill treadmill-running protocols. Blood lymphocytes isolated before, immediately after, 1h after, and 24h after each exercise test were assessed for markers of apoptosis (Annexin-V+, HSP60+), and CD55, CD59, and CD95 expression by flow cytometry. Lymphocytopenia occurred 1h after intensive and downhill running exercise, but no changes in the percentage of Annexin-V + or HSP60 + lymphocytes were found. Numbers of CD95+, CD55dim, and CD59dim lymphocytes increased immediately after intensive and downhill exercise, which were attributed to the selective mobilization and subsequent efflux of CD8+ and CD56+ lymphocyte subsets. No differences were found between the intensive and downhill protocols. In conclusion, apoptosis of circulating lymphocytes does not appear to contribute to exercise-induced lymphocytopenia.

Citation

Simpson, R. J., Florida-James, G., Whyte, G. P., Black, J., Ross, J., & Guy, K. (2007). Apoptosis does not contribute to the blood lymphocytopenia observed after intensive and downhill treadmill running in humans. Research in Sports Medicine, 15, 157-174. https://doi.org/10.1080/15438620701405339

• Journal Article Type: Article
• Publication Date: Jul 1, 2007
• Deposit Date: Jun 20, 2008
• Print ISSN: 1543-8627
• Electronic ISSN: 1543-8635
• Publisher: Routledge
• Peer Reviewed: Peer Reviewed
• Volume: 15
• Pages: 157-174
• DOI: https://doi.org/10.1080/15438620701405339
• Keywords: Lymphocytosis; CD antigens; Muscle damage; Heat-shock protein-60; Complement regulatory proteins; Flow cytometry
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/1636
• Publisher URL: http://dx.doi.org/10.1080/15438620701405339