Richard J. Simpson
Apoptosis Does not Contribute to the Blood Lymphocytopenia Observed After Intensive and Downhill Treadmill Running in Humans
Simpson, Richard J.; Florida-James, Geraint D.; Whyte, Greg P.; Black, James R.; Ross, James A.; Guy, Keith
Authors
Geraint D. Florida-James
Greg P. Whyte
James R. Black
James A. Ross
Keith Guy
Abstract
The lymphocytopenia that occurs during the recovery stage of exercise may be a result of apoptosis through an increased expression of CD95, a loss of the complement regulatory proteins CD55 and CD59, or both. Trained subjects completed intensive, moderate, and downhill treadmill-running protocols. Blood lymphocytes isolated before, immediately after, 1h after, and 24h after each exercise test were assessed for markers of apoptosis (Annexin-V+, HSP60+), and CD55, CD59, and CD95 expression by flow cytometry. Lymphocytopenia occurred 1h after intensive and downhill running exercise, but no changes in the percentage of Annexin-V + or HSP60 + lymphocytes were found. Numbers of CD95+, CD55dim, and CD59dim lymphocytes increased immediately after intensive and downhill exercise, which were attributed to the selective mobilization and subsequent efflux of CD8+ and CD56+ lymphocyte subsets. No differences were found between the intensive and downhill protocols. In conclusion, apoptosis of circulating lymphocytes does not appear to contribute to exercise-induced lymphocytopenia.
Citation
Simpson, R. J., Florida-James, G. D., Whyte, G. P., Black, J. R., Ross, J. A., & Guy, K. (2007). Apoptosis Does not Contribute to the Blood Lymphocytopenia Observed After Intensive and Downhill Treadmill Running in Humans. Research in Sports Medicine, 15(3), 157-174. https://doi.org/10.1080/15438620701405339
| Journal Article Type | Article |
|---|---|
| Online Publication Date | Nov 6, 2007 |
| Publication Date | Aug 28, 2007 |
| Deposit Date | Jun 20, 2008 |
| Journal | Research in Sports Medicine |
| Print ISSN | 1543-8627 |
| Electronic ISSN | 1543-8635 |
| Publisher | Routledge |
| Peer Reviewed | Peer Reviewed |
| Volume | 15 |
| Issue | 3 |
| Pages | 157-174 |
| DOI | https://doi.org/10.1080/15438620701405339 |
| Keywords | Lymphocytosis; CD antigens; Muscle damage; Heat-shock protein-60; Complement regulatory proteins; Flow cytometry |
| Public URL | http://researchrepository.napier.ac.uk/id/eprint/1636 |