Outputs (2382)

The Isolation of Genomic DNA from Invertebrates (1992)
Book Chapter
Vreugdenhil, E., & Darlison, M. G. (1992). The Isolation of Genomic DNA from Invertebrates. In A. Longstaff, & P. Revest (Eds.), Protocols in Molecular Neurobiology, 15-24. Springer Verlag. https://doi.org/10.1385/0-89603-199-3%3A15

Generally, genomic DNA is used either for the construction of genomic libraries or for Southern blot analysis. For several reasons, it is frequently preferable in the field of invertebrate neurobiology to screen, at least initially, genomic rather th... Read More about The Isolation of Genomic DNA from Invertebrates.

Molluscan ligand-gated ion-channel receptors (1993)
Book Chapter
Darlison, M. G., Hutton, M. L., & Harvey, R. J. (1993). Molluscan ligand-gated ion-channel receptors. In Comparative Molecular Neurobiology, 48-64. Springer Verlag. https://doi.org/10.1007/978-3-0348-7265-2_3

In this chapter we introduce the reader to the structures of the different types of ligand-gated ion-channel receptor, and the numerous receptor subtypes that have recently been revealed to exist, in both invertebrate and vertebrate species, by the a... Read More about Molluscan ligand-gated ion-channel receptors.

Molecular Characterization of GABA-Gated Chloride Ion Channels from Complex and Simple Nervous Systems (1994)
Book Chapter
Darlison, M. G. (1994). Molecular Characterization of GABA-Gated Chloride Ion Channels from Complex and Simple Nervous Systems. In C. Peracchia (Ed.), Handbook of Membrane Channels (303-315). Academic Press. https://doi.org/10.1016/b978-0-12-550640-3.50025-3

No abstract available.

Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate (1993)
Journal Article
Darlison, M. G., Bermudez, I., Van Minnen, J., Zimmermann, C., Amar, M., Dautzenberg, F. M., Kim, H., Stühmer, T., & Harvey, R. J. (1993). Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate. Netherlands Journal of Zoology, 44(3), 473-485. https://doi.org/10.1163/156854293x00557

Ligand-gated ion channels (also called ionotropic receptors) are a class of receptors which possess an intrinsic ion pore, selective for either cations or anions, that can be opened by the binding of an appropriate neurotransmitter. Ionotropic recept... Read More about Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate.

The Production of a Stably Transformed Insect Cell Line Expressing An Invertebrate GABA A Receptor β-Subunit (1995)
Journal Article
Smith, L. A., Amar, M., Harvey, R. J., Darlison, M. G., Earley, F. G. P., Beadle, D. J., King, L. A., & Bermudez, I. (1995). The Production of a Stably Transformed Insect Cell Line Expressing An Invertebrate GABA A Receptor β-Subunit. Journal of Receptors and Signal Transduction, 15(1-4), 33-41. https://doi.org/10.3109/10799899509045205

We have produced a stable insect cell line derived from Spodoptera frugiperda (Sf9) cells expressing a cDNA encoding a β-subunit of the Lymnaea stagnalis GABAA receptor. The cDNA was randomly integrated into the insect cell genome under the control o... Read More about The Production of a Stably Transformed Insect Cell Line Expressing An Invertebrate GABA A Receptor β-Subunit.

The embryonal carcinoma cell line PCC7-S-AzaR: 21.21: 21.2 (clone 1009) expresses a functional GABA: 21.2A: 21.2 receptor comprising the α5, β3 and γ3 subunits: 21.2 (1995)
Journal Article
Harvey, R. J., Lobron, C., Reinhardt-Maelicke, S., Maelicke, A., & Darlison, M. G. (1995). The embryonal carcinoma cell line PCC7-S-AzaR: 21.21: 21.2 (clone 1009) expresses a functional GABA: 21.2A: 21.2 receptor comprising the α5, β3 and γ3 subunits: 21.2. Behavioural Pharmacology, 6(SUPPLEMENT 1), 119. https://doi.org/10.1097/00008877-199505001-00138

GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness (1996)
Book Chapter
Darlison, M. G., & Harvey, R. J. (1996). GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness. In Molecular Biology of Membrane Transport Disorders (169-180). Springer. https://doi.org/10.1007/978-1-4613-1143-0_9

Rapid chemical communication between cells in the vertebrate central nervous system is mediated by ligand-gated ion-channel receptors (also called ionotropic receptors), which are multisubunit complexes, that each contain an ion-selective channel. In... Read More about GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness.

Transition to Postgraduate Study at Master’s Level: Lessons Learned from Students’ Evaluations of an Online Induction Resource (2020)
Journal Article
Robb, L., & Moffat, S. (2020). Transition to Postgraduate Study at Master’s Level: Lessons Learned from Students’ Evaluations of an Online Induction Resource. Journal of Perspectives in Applied Academic Practice, 8(1), 41-51. https://doi.org/10.14297/jpaap.v8i1.402

Evidence from UK universities’ Postgraduate Taught Experience Survey (PTES) concluded that whilst confident and experienced in their area of practice, some find the prospect of returning to study in an on-line environment and making the transition to... Read More about Transition to Postgraduate Study at Master’s Level: Lessons Learned from Students’ Evaluations of an Online Induction Resource.

The physiological demands of Gaelic football. (1995)
Journal Article
Florida-James, G., & Reilly, T. (1995). The physiological demands of Gaelic football. British Journal of Sports Medicine, 29(1), 41-45. doi:10.1136/bjsm.29.1.41

Match-lay demands of Gaelic football and fitness profiles were assessed at club competitive level. English Gaelic football club championship players (n = 11) were assessed for anthropometry, leg strength and time to exhaustion on a treadmill run. A s... Read More about The physiological demands of Gaelic football..

Lymphocyte Apoptosis, Adhesion/activation Molecules And Complement Regulatory Proteins Following Intensive, Moderate And Eccentric Exercise: 1739 11:30 AM - 11:45 AM (2005)
Journal Article
Simpson, R. J., Guy, K., Whyte, G. P., & Florida-James, G. D. (2005). Lymphocyte Apoptosis, Adhesion/activation Molecules And Complement Regulatory Proteins Following Intensive, Moderate And Eccentric Exercise: 1739 11:30 AM - 11:45 AM. Medicine and Science in Sports and Exercise, 37(Supplement), S336. https://doi.org/10.1097/00005768-200505001-01738

No abstract available.

Resting Blood Lymphocytes Are Activated By Autologous Serum After A 40km Cycling Time Trial: 542 (2009)
Journal Article
Simpson, R. J., Bartlett, D. B., Booth, S., Florida-James, G. D., & McFarlin, B. K. (2009). Resting Blood Lymphocytes Are Activated By Autologous Serum After A 40km Cycling Time Trial: 542. Medicine and Science in Sports and Exercise, 41(Supplement 1), 7. https://doi.org/10.1249/01.mss.0000353277.01938.3c

PURPOSE: Although it is known that peripheral blood lymphocytes display an activated phenotype immediately after an acute bout of high-intensity exercise, it is not known if these lymphocytes are activated by exercise or whether this is due to a mobi... Read More about Resting Blood Lymphocytes Are Activated By Autologous Serum After A 40km Cycling Time Trial: 542.

Perceived exertion and heart rate models for estimating metabolic workload in elite British soldiers performing a backpack load-carriage task (2010)
Journal Article
Simpson, R. J., Graham, S. M., Florida-James, G. D., Connaboy, C., Clement, R., & Jackson, A. S. (2010). Perceived exertion and heart rate models for estimating metabolic workload in elite British soldiers performing a backpack load-carriage task. Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme, 35(5), 650-656. https://doi.org/10.1139/h10-053

Identifying field measures to estimate backpack load-carriage work intensity in elite soldiers is of interest to the military. This study developed rating of perceived exertion (RPE) and heart rate models to define metabolic workload for a backpack l... Read More about Perceived exertion and heart rate models for estimating metabolic workload in elite British soldiers performing a backpack load-carriage task.

Lymphocyte Phenotype Alterations, Pro-Inflammatory Cytokines and Acute Phase Proteins Following Repeated Bouts of Mountainous Hill-Running: 2278 (2006)
Journal Article
Simpson, R. J., Guy, K., Whyte, G. P., Middleton, N., Black, J. R., Ross, J. A., Shave, R., & Florida-James, G. D. (2006). Lymphocyte Phenotype Alterations, Pro-Inflammatory Cytokines and Acute Phase Proteins Following Repeated Bouts of Mountainous Hill-Running: 2278. Medicine and Science in Sports and Exercise, 38(Supplement), S412???S413. https://doi.org/10.1249/00005768-200605001-01739

No abstract available.

High-Intensity Exercise Mobilises Senescent T-lymphocytes into the Peripheral Blood Compartment in Young and Old Subjects: 781 (2007)
Journal Article
Simpson, R. J., Cosgrove, C., Florida-James, G. D., Whyte, G. P., Pircher, H., & Guy, K. (2007). High-Intensity Exercise Mobilises Senescent T-lymphocytes into the Peripheral Blood Compartment in Young and Old Subjects: 781. Medicine and Science in Sports and Exercise, 39(Supplement), S61. https://doi.org/10.1249/01.mss.0000273140.61651.0b

T-cell senescence occurs during aging as a consequence of chromosome telomere shortening in response to repetitive antigenic stimulation. Senescent T-lymphocytes normally exhibit a “memory” phenotype (i.e. CD62L-/CD45RA-/CD45RO+) and express CD57 and... Read More about High-Intensity Exercise Mobilises Senescent T-lymphocytes into the Peripheral Blood Compartment in Young and Old Subjects: 781.

Physiological and Anthropometrical Indicators of Backpack Running Performance in ??Elite?? British Soldiers: 1384 (2007)
Journal Article
Graham, S. M., Simpson, R. J., Clement, R., & Florida-James, G. D. (2007). Physiological and Anthropometrical Indicators of Backpack Running Performance in ??Elite?? British Soldiers: 1384. Medicine and Science in Sports and Exercise, 39(Supplement), S199. https://doi.org/10.1249/01.mss.0000273743.89788.1f

PURPOSE: To identify indicators of backpack running performance in “elite” British soldiers using an extensive range of physiological and anthropometrical variables. METHODS: Thirteen male soldiers (mean ± SD: Age: 26 ± 4 yrs; VO2peak: 55.1 ± 5.4) f... Read More about Physiological and Anthropometrical Indicators of Backpack Running Performance in ??Elite?? British Soldiers: 1384.

Atrial Fibrillation Screen, Management And Guideline Recommended Therapy (AF SMART II) in the rural primary care setting: a cross-sectional study and cost-effectiveness analysis of eHealth tools to support all stages of screening (2020)
Journal Article
Orchard, J., Li, J., Freedman, B., Webster, R., Salkeld, G., Hespe, C., Gallagher, R., Patel, A., Kamel, B., Neubeck, L., & Lowres, N. (2020). Atrial Fibrillation Screen, Management And Guideline Recommended Therapy (AF SMART II) in the rural primary care setting: a cross-sectional study and cost-effectiveness analysis of eHealth tools to support all stages of screening. Journal of the American Heart Association JAHA, 9(18), Article e017080. https://doi.org/10.1161/JAHA.120.017080

Background Internationally, most atrial fibrillation (AF) management guidelines recommend opportunistic screening for AF in people aged ≥65 years, and oral anticoagulant (OAC) treatment for those at high stroke risk (CHA₂DS₂-VA ≥2). However, gaps re... Read More about Atrial Fibrillation Screen, Management And Guideline Recommended Therapy (AF SMART II) in the rural primary care setting: a cross-sectional study and cost-effectiveness analysis of eHealth tools to support all stages of screening.

Reliability Study Of The Technique To Assess Upper Respiratory Airway Inflammation In A Polluted Environment: 1695 (2009)
Journal Article
Gomes, E. C., Florida-James, G., & Stone, V. (2009). Reliability Study Of The Technique To Assess Upper Respiratory Airway Inflammation In A Polluted Environment: 1695. Medicine and Science in Sports and Exercise, 41(Supplement 1), 99. https://doi.org/10.1249/01.mss.0000354860.20764.82

Airway inflammation can be detected by an increased influx of inflammatory cells, such as neutrophils, as well as increased expression of inflammatory markers, which include interleukin-6 (IL-6) and Clara cell protein (CC16). When an individual is ex... Read More about Reliability Study Of The Technique To Assess Upper Respiratory Airway Inflammation In A Polluted Environment: 1695.

The MDM2 Ubiquitination Signal in the DNA-Binding Domain of p53 Forms a Docking Site for Calcium Calmodulin Kinase Superfamily Members (2007)
Journal Article
Craig, A. L., Chrystal, J. A., Fraser, J. A., Sphyris, N., Lin, Y., Harrison, B. J., Scott, M. T., Dornreiter, I., & Hupp, T. R. (2007). The MDM2 Ubiquitination Signal in the DNA-Binding Domain of p53 Forms a Docking Site for Calcium Calmodulin Kinase Superfamily Members. Molecular and Cellular Biology, 27(9), 3542-3555. https://doi.org/10.1128/mcb.01595-06

Genetic and biochemical studies have shown that Ser20 phosphorylation in the transactivation domain of p53 mediates p300-catalyzed DNA-dependent p53 acetylation and B-cell tumor suppression. However, the protein kinases that mediate this modification... Read More about The MDM2 Ubiquitination Signal in the DNA-Binding Domain of p53 Forms a Docking Site for Calcium Calmodulin Kinase Superfamily Members.

Drosophila melanogasterGlutamate-Cysteine Ligase Activity Is Regulated by a Modifier Subunit with a Mechanism of Action Similar to That of the Mammalian Form (2001)
Journal Article
Fraser, J. A., Saunders, R. D. C., & McLellan, L. I. (2002). Drosophila melanogasterGlutamate-Cysteine Ligase Activity Is Regulated by a Modifier Subunit with a Mechanism of Action Similar to That of the Mammalian Form. Journal of Biological Chemistry, 277(2), 1158-1165. https://doi.org/10.1074/jbc.m106683200

Glutamate-cysteine ligase (GCL) plays an important role in regulating glutathione homeostasis. In mammals, it comprises a catalytic (GCLC) and modifier (GCLM) subunit. The existence of a modifier subunit in invertebrates has not been described to dat... Read More about Drosophila melanogasterGlutamate-Cysteine Ligase Activity Is Regulated by a Modifier Subunit with a Mechanism of Action Similar to That of the Mammalian Form.

Antioxidant and cytoprotective responses to redox stress (2004)
Journal Article
Mathers, J., Fraser, J. A., McMahon, M., Saunders, R. D. C., Hayes, J. D., & McLellan, L. I. (2004). Antioxidant and cytoprotective responses to redox stress. Biochemical Society symposia, 71, 157-176. https://doi.org/10.1042/bss0710157

Aerobic cells produce reactive oxygen species as a consequence of normal cellular metabolism, and an array of antioxidant systems are in place to maintain the redox balance. When the redox equilibrium of the cell is upset by pro-oxidant environmental... Read More about Antioxidant and cytoprotective responses to redox stress.