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Genetic variability of Chlamydophila abortus strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genes (2011)
Journal Article
Saita, M., Clarka, E. M., Wheelhouse, N., Spalding, L., Livingstone, M., Sachse, K., Markey, B. K., Magnino, S., Siarkou, V. I., Vretou, E., Caro, M. R., Yaga, R., Lainson, F. A., Smith, D. G. E., Wright, F., & Longbottom, D. (2011). Genetic variability of Chlamydophila abortus strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genes. Veterinary Microbiology, 151(3-4), 284-290. https://doi.org/10.1016/j.vetmic.2011.03.005

This study used PCR-RFLP to investigate the genetic variability of pmp-encoding genes from fifty-two Chlamydophila abortus (C. abortus) strains originating from abortion cases from various geographical regions and host species. Six primer pairs were... Read More about Genetic variability of Chlamydophila abortus strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genes.

Chlamydia trachomatis infection increases fallopian tube PROKR2 via TLR2 and NFκB activation resulting in a microenvironment predisposed to ectopic pregnancy (2010)
Journal Article
Shaw, J. L. V., Wills, G. S., Lee, K., Horner, P. J., McClure, M. O., Abrahams, V. M., Wheelhouse, N., Jabbour, H. N., Critchley, H. O. D., Entrican, G., & Horne, A. W. (2011). Chlamydia trachomatis infection increases fallopian tube PROKR2 via TLR2 and NFκB activation resulting in a microenvironment predisposed to ectopic pregnancy. American Journal of Pathology, 178(1), 253-260. https://doi.org/10.1016/j.ajpath.2010.11.019

Chlamydia trachomatis and smoking are major risk factors for tubal ectopic pregnancy (EP), but the underlying mechanisms of these associations are not completely understood. Fallopian tube (FT) from women with EP exhibit altered expression of prokine... Read More about Chlamydia trachomatis infection increases fallopian tube PROKR2 via TLR2 and NFκB activation resulting in a microenvironment predisposed to ectopic pregnancy.

Ectopic lymphoid tissue formation in the lungs of mice infected with Chlamydia pneumoniae is associated with epithelial macrophage inflammatory protein-2/CXCL2 expression (2010)
Journal Article
Fitch, P., Wheelhouse, N., Bowles, P., Paterson, M., Longbottom, D., Entrican, G., & Howie, S. (2010). Ectopic lymphoid tissue formation in the lungs of mice infected with Chlamydia pneumoniae is associated with epithelial macrophage inflammatory protein-2/CXCL2 expression. Clinical and Experimental Immunology, 162(2), 372-378. https://doi.org/10.1111/j.1365-2249.2010.04231.x

Infection with Chlamydia pneumoniae (Cp) accounts for around 10% of community acquired bacterial pneumonia and has been associated with other chronic inflammatory conditions. We describe a C57/Bl6 murine model of Cp lung infection characterized by a... Read More about Ectopic lymphoid tissue formation in the lungs of mice infected with Chlamydia pneumoniae is associated with epithelial macrophage inflammatory protein-2/CXCL2 expression.

Immunological paradigms and the pathogenesis of ovine chlamydial abortion (2010)
Journal Article
Entrican, G., Wattegedera, S., Wheelhouse, N., Allan, A., & Rocchi, M. (2010). Immunological paradigms and the pathogenesis of ovine chlamydial abortion. American Journal of Reproductive Immunology, 64(4), 287-294. https://doi.org/10.1111/j.1600-0897.2010.00907.x

Successful mammalian pregnancy involves complex immunological interactions between the mother and foetus that are not yet fully understood. A number of immunological paradigms have been established to explain the failure of the maternal immune system... Read More about Immunological paradigms and the pathogenesis of ovine chlamydial abortion.

Expression of the repulsive SLIT/ROBO pathway in the human endometrium and Fallopian tube (2010)
Journal Article
Duncan, W. C., McDonald, S. E., Dickinson, R. E., Shaw, J. L. V., Lourenco, P. C., Wheelhouse, N., …Horne, A. W. (2010). Expression of the repulsive SLIT/ROBO pathway in the human endometrium and Fallopian tube. Molecular Human Reproduction, 16, 950-959. https://doi.org/10.1093/molehr/gaq055

We investigated whether the repulsive SLIT/ROBO pathway is expressed in the endometrium and is negatively regulated during implantation. We also examined whether deficient expression in the Fallopian tube (FT) may predispose to ectopic pregnancy (EP)... Read More about Expression of the repulsive SLIT/ROBO pathway in the human endometrium and Fallopian tube.

Evidence of Chlamydophila abortus vaccine strain 1B as a possible cause of ovine enzootic abortion (2010)
Journal Article
Wheelhouse, N., Aitchison, K., Laroucau, K., Thomson, J., & Longbottom, D. (2010). Evidence of Chlamydophila abortus vaccine strain 1B as a possible cause of ovine enzootic abortion. Vaccine, 28(35), 5657-5663. https://doi.org/10.1016/j.vaccine.2010.04.114

Chlamydophila abortus, the agent of ovine enzootic abortion (OEA), is a major cause of lamb mortality worldwide. Disease can be controlled through the use of vaccines based on the 1B temperature-sensitive mutant strain of C. abortus. This study inves... Read More about Evidence of Chlamydophila abortus vaccine strain 1B as a possible cause of ovine enzootic abortion.

Inflammatory cytokine responses in a pregnant mouse model of Chlamydophila abortus infection (2010)
Journal Article
Kerr, K., Wheelhouse, N., Livingstone, M., Anderson, I. E., Entrican, G., McKeever, D., & Longbottom, D. (2010). Inflammatory cytokine responses in a pregnant mouse model of Chlamydophila abortus infection. Veterinary Microbiology, 144(3-4), 392-398. https://doi.org/10.1016/j.vetmic.2010.01.025

Chlamydophila abortus (C. abortus) is the aetiological agent of ovine enzootic abortion (OEA). The highly elevated expression of the pro-inflammatory cytokine tumour necrosis factor-alpha (TNFα) and low-level expression of interferon-gamma (IFNγ) tha... Read More about Inflammatory cytokine responses in a pregnant mouse model of Chlamydophila abortus infection.

Transcriptional analysis of in vitro expression patterns of chlamydophila abortus polymorphic outer membrane proteins during the chlamydial developmental cycle. (2009)
Journal Article
Wheelhouse, N., Aitchison, K., Spalding, L., Livingstone, M., & Longbottom, D. (2009). Transcriptional analysis of in vitro expression patterns of chlamydophila abortus polymorphic outer membrane proteins during the chlamydial developmental cycle. Veterinary Research, 40(5), 47. https://doi.org/10.1051/vetres/2009030

Chlamydophila abortus is the aetiological agent of ovine enzootic abortion. Sequencing, annotation and comparative analysis of the genome of C. abortus strain S26/3 has revealed variation in the
loci encoding the polymorphic membrane proteins (Pmps)... Read More about Transcriptional analysis of in vitro expression patterns of chlamydophila abortus polymorphic outer membrane proteins during the chlamydial developmental cycle..

Ovine trophoblast is a primary source of TNFalpha during Chlamydophila abortus infection. (2009)
Journal Article
Wheelhouse, N., Wattegedera, S., Stanton, J., Maley, S., Watson, D., Jepson, C., Deane, D., Buxton, D., Longbottom, D., Baszler, T., & Entrican, G. (2009). Ovine trophoblast is a primary source of TNFalpha during Chlamydophila abortus infection. Journal of Reproductive Immunology, 80(1-2), 49-56. https://doi.org/10.1016/j.jri.2008.12.003

Chlamydophila abortus is a Gram-negative obligate intracellular bacterium that causes infectious abortion in sheep (ovine enzootic abortion, OEA) and humans. Infected placentas recovered from sheep that experience OEA have thickened membranes, contai... Read More about Ovine trophoblast is a primary source of TNFalpha during Chlamydophila abortus infection..

Frequency of the mitochondrial DNA 4977bp deletion in oesophageal mucosa during the progression of Barrett’s oesophagus (2009)
Journal Article
Tan, B., Skipworth, R., Stephens, N., Wheelhouse, N., Gilmour, H., De Beaux, A., Paterson-Brown, S., Fearon, K., & Ross, J. (2009). Frequency of the mitochondrial DNA 4977bp deletion in oesophageal mucosa during the progression of Barrett’s oesophagus. European Journal of Cancer, 45(5), 736-740. https://doi.org/10.1016/j.ejca.2009.01.013

Purpose: The mechanisms of the progression of Barrett’s oesophagus (BO) to oesophageal adenocarcinoma (OA) are poorly understood. The frequency of the 4977bp deletion in mitochondrial
DNA (mtDNA) was investigated in specimens ranging from normal oes... Read More about Frequency of the mitochondrial DNA 4977bp deletion in oesophageal mucosa during the progression of Barrett’s oesophagus.

Expression of secretory leukocyte protease inhibitor and elafin in human fallopian tube and in an in-vitro model of Chlamydia trachomatis infection (2008)
Journal Article
King, A. E., Wheelhouse, N., Cameron, S., McDonald, S. E., Lee, K., Entrican, G., …Horne, A. W. (2009). Expression of secretory leukocyte protease inhibitor and elafin in human fallopian tube and in an in-vitro model of Chlamydia trachomatis infection. Human Reproduction, 24(3), 679-686. https://doi.org/10.1093/humrep/den452

BACKGROUND: Secretory leukocyte protease inhibitor (SLPI) and elafin are anti-protease and anti-microbial molecules with a role in innate immune defence. They have been demonstrated at multiple mucosal surfaces including those of the female reproduct... Read More about Expression of secretory leukocyte protease inhibitor and elafin in human fallopian tube and in an in-vitro model of Chlamydia trachomatis infection.

Molecular detection of Chlamydophila abortus in post-abortion sheep at oestrus and subsequent lambing (2008)
Journal Article
Livingstone, M., Wheelhouse, N., Maley, S. W., & Longbottom, D. (2009). Molecular detection of Chlamydophila abortus in post-abortion sheep at oestrus and subsequent lambing. Veterinary Microbiology, 135(1-2), 134-141. https://doi.org/10.1016/j.vetmic.2008.09.033

Enzootic abortion of ewes (EAE), resulting from infection with the bacterium Chlamydophila abortus (C. abortus), is a major cause of lamb loss in Europe. The purpose of this study was to assess the potential impact of the shedding of organisms in pos... Read More about Molecular detection of Chlamydophila abortus in post-abortion sheep at oestrus and subsequent lambing.

Pregnancy, indoleamine 2,3-dioxygenase (IDO) and chlamydial abortion: an unresolved paradox (2008)
Journal Article
Entrican, G., Wattegedera, S., Rocchi, M., & Wheelhouse, N. (2009). Pregnancy, indoleamine 2,3-dioxygenase (IDO) and chlamydial abortion: an unresolved paradox. Veterinary Microbiology, 135(1-2), 98-102. https://doi.org/10.1016/j.vetmic.2008.09.028

Chlamydophila abortus infects the placental trophoblast in sheep, humans and mice, causing cell damage and inflammation that culminates in abortion. Host control of C. abortus appears to be heavily dependant on interferon (IFN)-gamma production. IFN-... Read More about Pregnancy, indoleamine 2,3-dioxygenase (IDO) and chlamydial abortion: an unresolved paradox.

Chlamydia trachomatis and Chlamydophila abortus induce the expression of secretory leukocyte protease inhibitor in cells of the human female reproductive tract (2008)
Journal Article
Wheelhouse, N., Wattegedera, S., Fleming, D., Fitch, P., Kelly, R., & Entrican, G. (2008). Chlamydia trachomatis and Chlamydophila abortus induce the expression of secretory leukocyte protease inhibitor in cells of the human female reproductive tract. Microbiology and Immunology, 52(9), 465-468. https://doi.org/10.1111/j.1348-0421.2008.00058.x

C. trachomatis and C. abortus are related Gram-negative intracellular bacteria that cause reproductive failure due to infertility (C. trachomatis) or abortion (C. abortus). These organisms target epithelial cells in the reproductive tract and/or plac... Read More about Chlamydia trachomatis and Chlamydophila abortus induce the expression of secretory leukocyte protease inhibitor in cells of the human female reproductive tract.

The tumor suppressor gene DLEC1 is frequently silenced by DNA methylation in hepatocellular carcinoma and induces G1 arrest in cell cycle (2007)
Journal Article
Qiu, G., Salto-Tellez, M., Ross, J. A., Yeo, W., Cui, Y., Wheelhouse, N., Chen, G. G., Harrison, D., Lai, P., Tao, Q., & Hooi, S. C. (2008). The tumor suppressor gene DLEC1 is frequently silenced by DNA methylation in hepatocellular carcinoma and induces G1 arrest in cell cycle. Journal of Hepatology, 48(3), 433-441. https://doi.org/10.1016/j.jhep.2007.11.015

Background/Aims: The chromosome locus 3p21.3 is a ‘‘hot-spot” for chromosomal aberrations and loss of heterozygosity in cancers. The 35 genes mapped to the AP20 subregion of this locus were screened for their expression to identify candidate tumor su... Read More about The tumor suppressor gene DLEC1 is frequently silenced by DNA methylation in hepatocellular carcinoma and induces G1 arrest in cell cycle.

Effect of high-fat enteral nutrition on hepatocyte injury in response to hemorrhagic shock in the rat (2007)
Journal Article
Chu, M. M., Luyer, M. D. P., Wheelhouse, N. M., Bellamy, C. O., Greve, J. W. M., Buurman, W. A., Sangster, K., Fearon, K. C. H., Ross, J. A., Garden, O. J., Dejong, C. H. C., & Wigmore, S. J. (2007). Effect of high-fat enteral nutrition on hepatocyte injury in response to hemorrhagic shock in the rat. World Journal of Surgery, 31(8), 1693-1701. https://doi.org/10.1007/s00268-007-9107-2

BACKGROUND: High-fat enteral nutrition reduces the inflammatory response following hemorrhagic shock in the rat. AIMS: We hypothesized that this intervention might also ameliorate the remote organ injury to the liver associated with this model. METHO... Read More about Effect of high-fat enteral nutrition on hepatocyte injury in response to hemorrhagic shock in the rat.

Differential expression of hDAB2IPA and hDAB2IPB in normal tissues and promoter methylation of hDAB2IPA in hepatocellular carcinoma (2006)
Journal Article
Qui, G., Xie, H., Wheelhouse, N., Harrison, D., Chen, G. G., Salto-Tellez, M., Lai, P., Ross, J. A., & Hooi, S. C. (2007). Differential expression of hDAB2IPA and hDAB2IPB in normal tissues and promoter methylation of hDAB2IPA in hepatocellular carcinoma. Journal of Hepatology, 46(4), 655-663. https://doi.org/10.1016/j.jhep.2006.11.012

BACKGROUND/AIMS: hDAB2IP is a candidate tumor suppressor gene. We studied the expression of its two variants, hDAB2IPA and hDAB2IPB, in normal tissues, and the expression and methylation status of hDAB2IPA in hepatocellular carcinomas (HCC) and cell... Read More about Differential expression of hDAB2IPA and hDAB2IPB in normal tissues and promoter methylation of hDAB2IPA in hepatocellular carcinoma.

The effects of macrophage migratory inhibitory factor on acute-phase protein production in primary human hepatocytes (2006)
Journal Article
Wheelhouse, N. M., Dowidar, N., Dejong, C. H. C., Garden, O. J., Powell, J. J., Barber, M. D., …Ross, J. A. (2006). The effects of macrophage migratory inhibitory factor on acute-phase protein production in primary human hepatocytes. International Journal of Molecular Medicine, 18(5), 957-961. https://doi.org/10.3892/ijmm.18.5.957

Macrophage inhibitory factor (MIF) is a pituitary peptide released during the physiological stress response, a T-cell product secreted during the antigen-specific response and a pro-inflammatory macrophage cytokine secreted after LPS stimulation. It... Read More about The effects of macrophage migratory inhibitory factor on acute-phase protein production in primary human hepatocytes.