Ectopic lymphoid tissue formation in the lungs of mice infected with Chlamydia pneumoniae is associated with epithelial macrophage inflammatory protein-2/CXCL2 expression

Fitch, Paul; Wheelhouse, Nick; Bowles, P; Paterson, M; Longbottom, David; Entrican, Gary; Howie, Sarah

Abstract

Infection with Chlamydia pneumoniae (Cp) accounts for around 10% of community acquired bacterial pneumonia and has been associated with other chronic inflammatory conditions. We describe a C57/Bl6 murine model of Cp lung infection characterized by a dose-dependent, resolving neutrophilia followed by lymphocytic infiltration of the lungs. By 21 days post-infection, mice exhibit a T helper type 1 (Th1) polarized serum antibody response with local mucosal antibody secretion and organization of ectopic lymphoid tissue which persisted in the absence of detectable Cp DNA. Macrophage inflammatory protein (MIP)-2/CXCL2, which recruits neutrophils and lymphocytes and is associated with ectopic lymphoid tissue formation, was secreted in the lungs post-infection. In vitro, lung epithelial cells up-regulated MIP-2/CXCL2 in response to both rough lipopolysaccharide (reLPS) and Cp infection. We conclude that Cp infection can have long-term inflammatory effects on tissue that persist after clearance of active infection.

Citation

Fitch, P., Wheelhouse, N., Bowles, P., Paterson, M., Longbottom, D., Entrican, G., & Howie, S. (2010). Ectopic lymphoid tissue formation in the lungs of mice infected with Chlamydia pneumoniae is associated with epithelial macrophage inflammatory protein-2/CXCL2 expression. Clinical and Experimental Immunology, 162(2), 372-378. https://doi.org/10.1111/j.1365-2249.2010.04231.x

• Journal Article Type: Article
• Acceptance Date: Jun 24, 2010
• Online Publication Date: Sep 14, 2010
• Publication Date: Oct 8, 2010
• Deposit Date: Jul 21, 2016
• Journal: Clin Exp Immunol
• Print ISSN: 0009-9104
• Electronic ISSN: 1365-2249
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 162
• Issue: 2
• Pages: 372-378
• DOI: https://doi.org/10.1111/j.1365-2249.2010.04231.x
• Keywords: Chlamydia pneumoniae, CXCL2/MIP-2, epithelium, lung, lymphocytes,
• Public URL: http://researchrepository.napier.ac.uk/Output/304717
• Related Public URLs: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996604/pdf/cei0162-0372.pdf