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Silver Nanoparticles: A Microbial Perspective (2011)
Book Chapter
Sweet, M., & Singleton, I. (2011). Silver Nanoparticles: A Microbial Perspective. . Academic Press. https://doi.org/10.1016/B978-0-12-387044-5.00005-4

Abstract Silver nanoparticles (NPs) are used for a wide range of commercial reasons to restrict microbial growth. The increasing use of silver {NPs} in modern materials ensures they will find their way into environmental systems. The mode of action w... Read More about Silver Nanoparticles: A Microbial Perspective.

Low-level atmospheric ozone exposure induces protection against Botrytis cinerea with down-regulation of ethylene-, jasmonate- and pathogenesis-related genes in tomato fruit (2011)
Journal Article
Tzortzakis, N., Taybi, T., Roberts, R., Singleton, I., Borland, A., & Barnes, J. (2011). Low-level atmospheric ozone exposure induces protection against Botrytis cinerea with down-regulation of ethylene-, jasmonate- and pathogenesis-related genes in tomat

The aim of this study was to determine if ozone exposure could prevent spoilage in tomato fruit by fungal infection and to explore concomitant changes in expression of genes involved in signal transduction (ethylene, jasmonic acid and C6-aldehydes) a... Read More about Low-level atmospheric ozone exposure induces protection against Botrytis cinerea with down-regulation of ethylene-, jasmonate- and pathogenesis-related genes in tomato fruit.

Effects of ozone exposure on the xerophilic fungus, Eurotium amstelodami IS-SAB-01, isolated from naan bread (2010)
Journal Article
Antony-Babu, S., & Singleton, I. (2011). Effects of ozone exposure on the xerophilic fungus, Eurotium amstelodami IS-SAB-01, isolated from naan bread. International Journal of Food Microbiology, 144(3), 331-336. https://doi.org/10.1016/j.ijfoodmicro.2010.

Xerophilic moulds cause contamination and spoilage of low moisture foods. This study examined the effect of ozone fumigation on growth of a Eurotium species isolated from naan bread. Two ozone treatments were used — a low-level long-term exposure (0.... Read More about Effects of ozone exposure on the xerophilic fungus, Eurotium amstelodami IS-SAB-01, isolated from naan bread.

Quinol-cytochrome c Oxidoreductase and Cytochrome c4 Mediate Electron Transfer during Selenate Respiration in Thauera selenatis (2010)
Journal Article
Lowe, E. C., Bydder, S., Hartshorne, R. S., Tape, H. L. U., Dridge, E. J., Debieux, C. M., …Butler, C. S. (2010). Quinol-cytochrome c Oxidoreductase and Cytochrome c4 Mediate Electron Transfer during Selenate Respiration in Thauera selenatis. Journal of

Selenate reductase (SER) from Thauera selenatis is a periplasmic enzyme that has been classified as a type II molybdoenzyme. The enzyme comprises three subunits SerABC, where SerC is an unusual b-heme cytochrome. In the present work the spectropotent... Read More about Quinol-cytochrome c Oxidoreductase and Cytochrome c4 Mediate Electron Transfer during Selenate Respiration in Thauera selenatis.

Effect of ozone on spore germination, spore production and biomass production in two Aspergillus species (2009)
Journal Article
Antony-Babu, S., & Singleton, I. (2009). Effect of ozone on spore germination, spore production and biomass production in two Aspergillus species. Antonie van Leeuwenhoek, 96(4), 413-422. https://doi.org/10.1007/s10482-009-9355-2

The ability of ozone gas to reduce food spoilage is relatively well documented, but the developmental effects of the gas on food spoilage fungi are not well known. In this study two model aspergilli, Aspergillus nidulans and Aspergillus ochraceus wer... Read More about Effect of ozone on spore germination, spore production and biomass production in two Aspergillus species.

Potential use of DNA adducts to detect mutagenic compounds in soil (2008)
Journal Article
Hua, G., Lyons, B., Killham, K., & Singleton, I. (2009). Potential use of DNA adducts to detect mutagenic compounds in soil. Environmental Pollution, 157(3), 916-921. https://doi.org/10.1016/j.envpol.2008.10.026

In this study, three different soils with contrasting features, spiked with 300 mg benzo[a]pyrene (BaP)/kg dry soil, were incubated at 20 °C and 60% water holding capacity for 540 days. At different time points, BaP and {DNA} were extracted and quant... Read More about Potential use of DNA adducts to detect mutagenic compounds in soil.

Biological tools to assess contaminant bioavailability in soils (2008)
Book Chapter
Keatinge, Z., & Singleton, I. (2008). Biological tools to assess contaminant bioavailability in soils. In Chemical Bioavailability in Terrestrial Environment; Developments in Soil Science (479-494). Elsevier. https://doi.org/10.1016/s0166-2481%2807%29320

This chapter provides an overview of the biological tools to assess contaminants bioavailability in soils. Compounds that cause contamination include heavy metals, polyaromatic hydrocarbons (PAHs), pesticides, and chlorinated solvents. For the purpos... Read More about Biological tools to assess contaminant bioavailability in soils.

Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit (2007)
Journal Article
Tzortzakis, N., Singleton, I., & Barnes, J. (2008). Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit. Postharvest Biology and Technology, 47(1), 1-9. https://doi.org/10.1016/j.postharvbio.2007.06.004

Tomato fruit (Lycopersicon esculentum L.) were exposed to ozone concentrations between 0.005 (control) and 5.0 μmol mol−1 up to 13 days at 13 °C, prior to, or following, inoculation by Alternaria alternata or Colletotrichum coccodes (causes of black... Read More about Impact of low-level atmospheric ozone-enrichment on black spot and anthracnose rot of tomato fruit.

Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit (2007)
Journal Article
Tzortzakis, N., Borland, A., Singleton, I., & Barnes, J. (2007). Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit. Postharvest Biology and Technology, 45(3), 317-325. https://doi.org/10.1016/j.postharvbio.2007.03.004

Tomato fruit (Lycopersicon esculentum L. cv. Carousel) were exposed to ozone concentrations ranging between 0.005 (controls) and 1.0 μmol mol−1 at 13 °C and 95% RH. Quality-related attributes and organoleptic characteristics were examined during and... Read More about Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit.

Deployment of low-level ozone-enrichment for the preservation of chilled fresh produce (2007)
Journal Article
Tzortzakis, N., Singleton, I., & Barnes, J. (2007). Deployment of low-level ozone-enrichment for the preservation of chilled fresh produce. Postharvest Biology and Technology, 43(2), 261-270. https://doi.org/10.1016/j.postharvbio.2006.09.005

Tomatoes, strawberries, table grapes and plums were inoculated with Botrytis cinerea (grey mould), transferred to chilled storage (13 °C) and exposed to ‘clean air’ or low-level ozone-enrichment (0.1 μmol mol−1). Ozone-enrichment resulted in a substa... Read More about Deployment of low-level ozone-enrichment for the preservation of chilled fresh produce.

Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-β-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS) (2007)
Journal Article
Hua, G., Broderick, J., Semple, K. T., Killham, K., & Singleton, I. (2007). Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-β-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS). Environmental Pollut

Synchronous fluorescence spectroscopy (SFS) was directly applied to rapidly quantify selected polycyclic aromatic hydrocarbons (PAHs: benzo[a]pyrene and pyrene) in aqueous hydroxypropyl-β-cyclodextrin (HPCD) soil extract solutions from a variety of a... Read More about Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-β-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS).

Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting (2006)
Journal Article
Vizard, C. G., Rimmer, D. L., Pless-Mulloli, T., Singleton, I., & Air, V. S. (2006). Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting. Science of the T

A study of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/F) concentrations was undertaken in the vicinity of a municipal solid waste incinerator (MSWI) in Newcastle upon Tyne as a result of concerns raised by local re... Read More about Identifying contemporary and historic sources of soil polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in an industrial urban setting.

Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid. (2006)
Journal Article
Lee, M., Kim, M., Singleton, I., Goodfellow, M., & Lee, S. (2006). Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid. Journal of Applied Microbiology, 100(2), 325-333. https://doi.org

Aims:  The aim of the present study was to isolate and characterize a bacterium, strain EN3, capable of using diesel oil as a major carbon and energy source, and to analyse the enhancement of diesel oil degradation by this organism using synthetic my... Read More about Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid..

Environmental Pollution and Restoration: A Role for Bioremediation (2005)
Book Chapter
Philp, J. C., Bamforth, S., Singleton, I., & Atlas, R. M. (2005). Environmental Pollution and Restoration: A Role for Bioremediation. In R. M. Atlas, & J. Philp (Eds.), Bioremediation, 1-48. American Society for Microbiology. doi:10.1128/9781555817596.ch1

With greater understanding of microbial diversity and the development of bioengineering, bioremediation is taking its place as a cost-effective technique in integrated environmental restoration efforts. The major reasons for the control of water and... Read More about Environmental Pollution and Restoration: A Role for Bioremediation.

The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil. (2005)
Journal Article
O’Mahony, M. M., Dobson, A. D., Barnes, J. D., & Singleton, I. (2006). The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil. Chemosphere, 63(2), 307-314. https://doi.org/10.1016/j.chemosphere.2005.07.018

The potential of using ozone for the removal of phenanthrene from several different soils, both alone and in combination with biodegradation using a microbial inoculant (Pseudomonas alcaligenes PA-10), was examined. The greater the water content of t... Read More about The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil..

Metal contamination of urban soils in the vicinity of a municipal waste incinerator: one source among many. (2005)
Journal Article
Rimmer, D. L., Vizard, C. G., Pless-Mulloli, T., Singleton, I., Air, V. S., & Keatinge, Z. A. (2006). Metal contamination of urban soils in the vicinity of a municipal waste incinerator: one source among many. Science of the Total Environment, 356(1-3), 207-216. https://doi.org/10.1016/j.scitotenv.2005.04.037

Concern from local residents about possible contamination with metals and PCDD/F (dioxins and furans) from fugitive and stack emissions from the Byker municipal solid waste incinerator in Newcastle upon Tyne led the City Council to initiate a study o... Read More about Metal contamination of urban soils in the vicinity of a municipal waste incinerator: one source among many..

DDT Resistance and Transformation by Different Microbial Strains Isolated from DDT-Contaminated Soils and Compost Materials (2003)
Journal Article
Kantachote, D., Singleton, I., McClure, N., Naidu, R., Megharaj, M., & Harch, B. D. (2003). DDT Resistance and Transformation by Different Microbial Strains Isolated from DDT-Contaminated Soils and Compost Materials. Compost science & utilization, 11(4), 300-310. https://doi.org/10.1080/1065657x.2003.10702139

Bioremediation is a potential option to treat 1, 1, 1-trichloro-2, 2-bis (4-chlorophenyl) ethane (DDT) contaminated sites. In areas where suitable microbes are not present, the use of DDT resistant microbial inoculants may be necessary. It is vital t... Read More about DDT Resistance and Transformation by Different Microbial Strains Isolated from DDT-Contaminated Soils and Compost Materials.

Changes in microbial properties associated with long-term arsenic and DDT contaminated soils at disused cattle dip sites (2003)
Journal Article
Edvantoro, B., Naidu, R., Megharaj, M., & Singleton, I. (2003). Changes in microbial properties associated with long-term arsenic and DDT contaminated soils at disused cattle dip sites. Ecotoxicology and Environmental Safety, 55(3), 344-351. https://doi.org/10.1016/s0147-6513%2802%2900092-1

This work examined the effect of long-term arsenic (As) and DDT contamination on soil microbial properties at 11 cattle dip sites in northern New South Wales, Australia. Total As in the surface (0–10 cm) soils from these sites ranged from 34 to 2941... Read More about Changes in microbial properties associated with long-term arsenic and DDT contaminated soils at disused cattle dip sites.

Fungal remediation of soils contaminated with persistent organic pollutants (2001)
Book Chapter
Singleton, I. (2001). Fungal remediation of soils contaminated with persistent organic pollutants. In G. M. Gadd (Ed.), Fungi in Bioremediation, (79-96). Cambridge: Cambridge University Press. https://doi.org/10.1017/CBO9780511541780.005

Laboratory-based studies have shown that fungi are able to degrade a wide range of organic pollutants (see other chapters) and have great potential for use as inoculants to remediate contaminated soil. However, soil is a heterogeneous environment and... Read More about Fungal remediation of soils contaminated with persistent organic pollutants.