Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-β-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS)

Hua, Guoxiong; Broderick, John; Semple, Kirk T.; Killham, Ken; Singleton, Ian

Abstract

Synchronous fluorescence spectroscopy (SFS) was directly applied to rapidly quantify selected polycyclic aromatic hydrocarbons (PAHs: benzo[a]pyrene and pyrene) in aqueous hydroxypropyl-β-cyclodextrin (HPCD) soil extract solutions from a variety of aged contaminated soils containing four different PAHs. The method was optimized and validated. The results show that {SFS} can be used to analyse benzo[a]pyrene and pyrene in {HPCD} based soil extracts with high sensitivity and selectivity. The linear calibration ranges were 4.0 × 10−6–1.0 × 10−3 mM for benzo[a]pyrene and 6.0 × 10−6–1.2 × 10−3 mM for pyrene in 10 mM {HPCD} aqueous solution alone. The detection limits according to the error propagation theory for benzo[a]pyrene and pyrene were 3.9 × 10−6 and 5.4 × 10−6 mM, respectively. A good agreement between {SFS} and {HPLC} was reached for both determinations of {PAHs} in {HPCD} alone and in soil {HPCD} extracts. Hence, {SFS} is a potential means to simplify the present non-exhaustive hydroxypropyl-β-cyclodextrin (HPCD)-based extraction technique for the evaluation of {PAH} bioavailability in soil.

• Journal Article Type: Article
• Acceptance Date: Oct 21, 2006
• Online Publication Date: Jan 19, 2007
• Publication Date: 2007-07
• Deposit Date: Jul 27, 2016
• Journal: Environmental Pollution
• Print ISSN: 0269-7491
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 148
• Issue: 1
• Pages: 176-181
• DOI: https://doi.org/10.1016/j.envpol.2006.10.040
• Keywords: Benzo[a]pyrene; Pyrene; Bioavailability; Hydroxypropyl-β-cyclodextrin (HPCD); Synchronous fluorescence spectrometry (SFS); Soil; Bioremediation
• Public URL: http://researchrepository.napier.ac.uk/Output/318463
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0269749106005951