Potential use of DNA adducts to detect mutagenic compounds in soil

Hua, Guoxiong; Lyons, Brett; Killham, Ken; Singleton, Ian

Abstract

In this study, three different soils with contrasting features, spiked with 300 mg benzo[a]pyrene (BaP)/kg dry soil, were incubated at 20 °C and 60% water holding capacity for 540 days. At different time points, BaP and {DNA} were extracted and quantified, and {DNA} adducts were quantified by 32P-postlabelling. After 540 days incubation, 69.3, 81.6 and 83.2% of initial BaP added remained in Cruden Bay, Boyndie and Insch soils, respectively. Meanwhile, a significantly different amount of DNA–BaP adducts were found in the three soils exposed to BaP over time. The work demonstrates the concept that {DNA} adducts can be detected on {DNA} extracted from soil. Results suggest the technique is not able to directly reflect bioavailability of BaP transformation products. However, this new method provides a potential way to detect mutagenic compounds in contaminated soil and to assess the outcomes of soil remediation.

Citation

Hua, G., Lyons, B., Killham, K., & Singleton, I. (2009). Potential use of DNA adducts to detect mutagenic compounds in soil. Environmental Pollution, 157(3), 916-921. https://doi.org/10.1016/j.envpol.2008.10.026

• Journal Article Type: Article
• Acceptance Date: Oct 29, 2008
• Online Publication Date: Dec 11, 2008
• Publication Date: 2009-03
• Deposit Date: Jul 27, 2016
• Journal: Environmental Pollution
• Print ISSN: 0269-7491
• Electronic ISSN: 1873-6424
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 157
• Issue: 3
• Pages: 916-921
• DOI: https://doi.org/10.1016/j.envpol.2008.10.026
• Keywords: Polycyclic aromatic hydrocarbons (PAHs); DNA-PAH adducts; 32P-postlabelling assay; Soil pollution
• Public URL: http://researchrepository.napier.ac.uk/Output/318486
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0269749108005861