Effects of long-term contamination of DDT on soil microflora with special reference to soil algae and algal transformation of DDT

Megharaj, M; Kantachote, D; Singleton, I; Naidu, R

Abstract

DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane) and its principle metabolites, DDE (1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene) and DDD (1,1-dichloro-2,2-bis(p-chlorophenyl)ethane) are widespread environmental contaminants but little information is available concerning their effects on non-target microflora (especially microalgae and cyanobacteria) and their activities in long-term contaminated soils. For this reason a long-term DDT-contaminated soil was screened for DDT residues and toxicity to microorganisms (bacteria, fungi, algae), microbial biomass and dehydrogenase activity. Also, five pure cultures isolated from various sites (two unicellular green algae and three dinitrogen-fixing cyanobacteria) were tested for their ability to metabolise DDT. Viable counts of bacteria and algae declined with increasing DDT contamination while fungal counts, microbial biomass and dehydrogenase activity increased in medium-level contaminated soil (27 mg DDT residues kg−1 soil). All the tested parameters were greatly inhibited in high-level contaminated soil (34 mg DDT residues kg−1 soil). Species composition of algae and cyanobacteria was altered in contaminated soils and sensitive species were eliminated in the medium and high contaminated soils suggesting that these organisms could be useful as bioindicators of pollution. Microbial biomass and dehydrogenase activity may not serve as good bioindicators of pollution since these parameters were potentially influenced by the increase in fungal (probably DDT resistant) counts. All the tested algal species metabolised DDT to DDE and DDD; however, transformation to DDD was more significant in the case of dinitrogen-fixing cyanobacteria.

Citation

Megharaj, M., Kantachote, D., Singleton, I., & Naidu, R. (2000). Effects of long-term contamination of DDT on soil microflora with special reference to soil algae and algal transformation of DDT. Environmental Pollution, 109(1), 35-42. https://doi.org/10.1016/s0269-7491%2899%2900231-6

• Journal Article Type: Article
• Acceptance Date: Aug 4, 1999
• Online Publication Date: Mar 29, 2000
• Publication Date: 2000-07
• Deposit Date: Aug 5, 2016
• Journal: Environmental Pollution
• Print ISSN: 0269-7491
• Electronic ISSN: 1873-6424
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 109
• Issue: 1
• Pages: 35-42
• DOI: https://doi.org/10.1016/s0269-7491%2899%2900231-6
• Keywords: Soil algae, Microorganisms, DDT residues, Biodegradation, Toxicity,
• Public URL: http://researchrepository.napier.ac.uk/Output/328284