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In an ovine model of polycystic ovary syndrome (PCOS) prenatal androgens suppress female fetal renal gluconeogenesis.

Kanellopoulos-Langevin, Colette; Connolly, Fiona; Rae, Mick T; Sp�th, Katharina; Boswell, Lyndsey; McNeilly, Alan S.; Duncan, W. Colin


Colette Kanellopoulos-Langevin

Fiona Connolly

Katharina Sp�th

Lyndsey Boswell

Alan S. McNeilly

W. Colin Duncan


Increased maternal androgen exposure during pregnancy programmes a polycystic ovary syndrome (PCOS)-like condition, with metabolic dysfunction, in adult female offspring. Other in utero exposures associated with the development of insulin resistance, such as intrauterine growth restriction and exposure to prenatal glucocorticoids, are associated with altered fetal gluconeogenesis. We therefore aimed to assess the effect of maternal androgenisation on the expression of PEPCK and G6PC in the ovine fetus. Pregnant Scottish Greyface sheep were treated with twice weekly testosterone propionate (TP; 100mg) or vehicle control from day 62 to day102 of gestation. At day 90 and day 112 fetal plasma and liver and kidney tissue was collected for analysis. PEPCK and G6PC expression were analysed by quantitative RT-PCR, immunohistochemistry and western blotting. PEPCK and G6PC were localised to fetal hepatocytes but maternal androgens had no effect on female or male fetuses. PEPCK and G6PC were also localised to the renal tubules and renal PEPCK (P


Kanellopoulos-Langevin, C., Connolly, F., Rae, M. T., Späth, K., Boswell, L., McNeilly, A. S., & Duncan, W. C. (2015). In an ovine model of polycystic ovary syndrome (PCOS) prenatal androgens suppress female fetal renal gluconeogenesis. PLOS ONE, 10, Article e0132113.

Journal Article Type Article
Acceptance Date Jun 10, 2015
Online Publication Date Jul 6, 2015
Publication Date Jul 6, 2015
Deposit Date Oct 27, 2015
Publicly Available Date Aug 26, 2019
Print ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 10
Article Number e0132113
Keywords Maternal androgen exposure; ovary syndrome (PCOS)-like condition; metabolic dysfunction; in utero exposures;
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In An Ovine Model Of Polycystic Ovary Syndrome (PCOS) Prenatal Androgens Suppress Female Fetal Renal Gluconeogenesis (2.9 Mb)

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Copyright Statement
© 2015 Connolly et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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