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Identification of a dominant negative functional domain on DAPK-1 that degrades DAPK-1 protein and stimulates TNFR-1-mediated apoptosis.

Lin, Yao; Stevens, Craig; Hupp, Ted

Authors

Yao Lin

Ted Hupp



Abstract

DAPK-1 is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. To define mechanisms of DAPK-1 protein regulation, we have determined that DAPK-1 protein has a long half-life, and therefore its activity is primarily regulated at the protein level. Changes in DAPK-1 protein levels occur by a cathepsin B-dependent pathway, prompting us to evaluate whether cathepsin B plays positive or negative role in DAPK-1 function. The transfection of p55-TNFR-1 induced complex formation between DAPK-1 and cathepsin B. Depletion of cathepsin B protein using small interfering RNA stimulated TNFR-1 dependent apoptosis. The minimal binding region on DAPK-1 for cathepsin B was mapped to amino acids 836–947. The transfection of the DAPK-1-(836–947) miniprotein acted in a dominant negative manner inducing endogenous DAPK-1 protein degradation in a TNFR-1-dependent manner. These data suggest that DAPK-1 forms a multiprotein survival complex with cathepsin B countering the rate of TNFR-1-dependent apoptosis and highlights the importance of developing DAPK-1 inhibitors as agents to sensitize cells to stress-induced apoptosis.

Citation

Lin, Y., Stevens, C., & Hupp, T. (2007). Identification of a dominant negative functional domain on DAPK-1 that degrades DAPK-1 protein and stimulates TNFR-1-mediated apoptosis. Journal of Biological Chemistry, 282(23), 16792-16802. https://doi.org/10.1074/jbc.M611559200

Journal Article Type Article
Online Publication Date Feb 26, 2007
Publication Date Jun 8, 2007
Deposit Date Aug 2, 2016
Journal Journal of Biological Chemistry
Print ISSN 0021-9258
Electronic ISSN 1083-351X
Publisher American Society for Biochemistry and Molecular Biology
Peer Reviewed Peer Reviewed
Volume 282
Issue 23
Pages 16792-16802
DOI https://doi.org/10.1074/jbc.M611559200
Keywords DAPK-1, tumor suppressor protein, signal transduction pathways, protein regulation,
Public URL http://researchrepository.napier.ac.uk/Output/322308