Conserved Organization of ?-Aminobutyric AcidAReceptor Genes: Cloning and Analysis of the Chicken ?4-Subunit Gene

Lasham, Annette; Vreugdenhil, Erno; Bateson, Alan N.; Barnard, Eric A.; Darlison, Mark G.

Abstract

Abstract: A series of genomic clones containing DNA that encodes the chicken γ-aminobutyric acidA (GABAA) receptor β4 subunit have been isolated. These have been restriction mapped and partially sequcnced to determine the structural organization and the size of the β4-subunit gene. This gene, which comprises nine exons, spans more than 65 kb. The organization of the chicken GABAA receptor β4-subunit gene has been compared to that of the murine GABAA receptor δ-subunit gene and to those of the genes that encode other members of the ligand-gated ion-channel superfamily, namely muscle and neuronal nicotinic acetylcholine receptors (AChRs). Although the positions of the intron/exon boundaries of GABAA receptor sub-unit genes are seen to be highly conserved, there are significant differences between the genes that encode GABAA receptor and AChR subunits. These results are discussed in relation to the proposal that this superfamily of ligand-gated ion-channel receptor genes arose by duplication of an ancestral receptor gene.

Citation

Lasham, A., Vreugdenhil, E., Bateson, A. N., Barnard, E. A., & Darlison, M. G. (1991). Conserved Organization of ?-Aminobutyric AcidAReceptor Genes: Cloning and Analysis of the Chicken ?4-Subunit Gene. Journal of Neurochemistry, 57(1), 352-355. https://doi.org/10.1111/j.1471-4159.1991.tb02135.x

• Journal Article Type: Article
• Publication Date: 1991-07
• Deposit Date: Jul 27, 2016
• Journal: Journal of Neurochemistry
• Print ISSN: 0022-3042
• Electronic ISSN: 1471-4159
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 57
• Issue: 1
• Pages: 352-355
• DOI: https://doi.org/10.1111/j.1471-4159.1991.tb02135.x
• Keywords: γ‐Aminobutyric acid A receptor, Chicken β4 subunit, Gene organization, Ligand‐gated ion channel, Receptor superfamily, Evolution,
• Public URL: http://researchrepository.napier.ac.uk/Output/317637
• Publisher URL: http://dx.doi.org/10.1111/j.1471-4159.1991.tb02135.x