Dumitru Sirbu
Locally Excited State–Charge Transfer State Coupled Dyes as Optically Responsive Neuron Firing Probes
Sirbu, Dumitru; Butcher, John B; Waddell, Paul G; Andras, Peter; Benniston, Andrew C
Authors
John B Butcher
Paul G Waddell
Prof Peter Andras P.Andras@napier.ac.uk
Dean of School of Computing Engineering and the Built Environment
Andrew C Benniston
Abstract
A selection of NIR-optically responsive neuron probes was produced comprising of a donor julolidyl group connected to a BODIPY core and several different styryl and vinylpyridinyl derived acceptor moieties. The strength of the donor–acceptor interaction was systematically modulated by altering the electron withdrawing nature of the aryl unit. The fluorescence quantum yield was observed to decrease as the electron withdrawing effect of the aryl subunit increased in line with changes of the Hammett parameter. The effectiveness of these fluorophores as optically responsive dyes for neuronal imaging was assessed by measuring the toxicity and signal-to-noise ratio (SNR) of each dye. A great improvement of SNR was obtained when compared to the first-generation BODIPY-based voltage sensitive dyes with concomitant toxicity decrease. The mechanism for the optical response is disparate from conventional cyanine-based dyes, opening up a new way to produce effective voltage sensitive dyes that respond well into the NIR region.
Citation
Sirbu, D., Butcher, J. B., Waddell, P. G., Andras, P., & Benniston, A. C. (2017). Locally Excited State–Charge Transfer State Coupled Dyes as Optically Responsive Neuron Firing Probes. Chemistry - A European Journal, 23(58), 14639-14649. https://doi.org/10.1002/chem.201703366
Journal Article Type | Article |
---|---|
Online Publication Date | Sep 18, 2017 |
Publication Date | 2017-10 |
Deposit Date | Nov 8, 2021 |
Journal | Chemistry-A European Journal |
Print ISSN | 0947-6539 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
Volume | 23 |
Issue | 58 |
Pages | 14639-14649 |
DOI | https://doi.org/10.1002/chem.201703366 |
Keywords | BODIPY dyes, fluorescence, neuron, structure–reactivity relationship, voltage response |
Public URL | http://researchrepository.napier.ac.uk/Output/2808872 |
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