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A rapid screening assay for identifying mycobacteria targeted nanoparticle antibiotics

Donnellan, Samantha; Tran, Lang; Johnston, Helinor; McLuckie, Joyce; Stevenson, Karen; Stone, Vicki

Authors

Lang Tran

Helinor Johnston

Joyce McLuckie

Karen Stevenson

Vicki Stone



Abstract

Antibiotic resistance is a serious problem. Nanotechnology offers enormous potential in medicine, yet there is limited knowledge regarding the toxicity of nanoparticles (NP) for mycobacterial species that cause serious human diseases (e.g. tuberculosis (TB) and leprosy). Mycobacterial diseases are a major global health problem; TB caused by Mycobacterium tuberculosis (Mtb) kills up to 2 million people annually and there are over 200 000 leprosy cases each year caused by Mycobacterium leprae (M. leprae). Few drugs are effective against these mycobacteria and increasing antibiotic resistance exacerbates the problem. As such, alternative therapies are urgently needed but most current assays used to assess the effectiveness of therapeutics against mycobacteria are slow and expensive. This study aimed to develop a rapid, low-cost assay which can be used for screening the antimicrobial properties of compounds against pathogenic mycobacteria and to assess the toxicity of three NP (silver [Ag], copper oxide [Cu(II)O], and zinc oxide [ZnO]) against a green fluorescent protein reporter strain of Mycobacterium avium subspecies paratuberculosis, a slow growing, pathogenic mycobacterial species causing paratuberculosis in ruminants. Fluorescence was used to monitor mycobacterial growth over time, with NP concentrations of 6.25–100 μg/mL tested for up to 7 days, and a method of data analysis was designed to permit comparison between results. Mycobacterial sensitivity to the NP was found to be NP composition specific and toxicity could be ranked in the following order: Ag > Cu(II)O > ZnO.

Citation

Donnellan, S., Tran, L., Johnston, H., McLuckie, J., Stevenson, K., & Stone, V. (2016). A rapid screening assay for identifying mycobacteria targeted nanoparticle antibiotics. Nanotoxicology, 10(6), 761-769. https://doi.org/10.3109/17435390.2015.1124468

Journal Article Type Article
Acceptance Date Nov 19, 2015
Online Publication Date Feb 5, 2016
Publication Date Jul 2, 2016
Deposit Date Feb 11, 2021
Journal Nanotoxicology
Print ISSN 1743-5390
Electronic ISSN 1743-5404
Publisher Taylor & Francis
Peer Reviewed Peer Reviewed
Volume 10
Issue 6
Pages 761-769
DOI https://doi.org/10.3109/17435390.2015.1124468
Keywords Antibacterial activity, antibiotic resistance, drug screening, mycobacterium, nano medicine
Public URL http://researchrepository.napier.ac.uk/Output/2718408