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A Quantitative Method for the Study of HIV-1 and Mycobacterium tuberculosis Coinfection

Donnellan, Samantha; Pennington, Shaun H.; Ruggiero, Alessandra; Martinez-Rodriguez, Carmen; Pouget, Marion; Thomas, Jordan; Ward, Steve A.; Pollakis, Georgios; Biagini, Giancarlo A.; Paxton, William A.

Authors

Shaun H. Pennington

Alessandra Ruggiero

Carmen Martinez-Rodriguez

Marion Pouget

Jordan Thomas

Steve A. Ward

Georgios Pollakis

Giancarlo A. Biagini

William A. Paxton



Abstract

Mycobacterium tuberculosis and human immunodeficiency virus-1 (HIV-1) syndemic interactions are a major global health concern. Despite the clinical significance of coinfection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of coinfection is limited. Here, we use single-round infectious HIV-1 pseudotyped viral particles expressing green fluorescent protein alongside M. tuberculosis expressing mCherry to study pathogenesis and treatment. We report that HIV-1 infection inhibited intracellular replication of M. tuberculosis and demonstrate the therapeutic activity of antiviral treatment (efavirenz) and antimicrobial treatment (rifampicin). The described method could be applied for detailed mechanistic studies to inform the development of novel treatment strategies.

Citation

Donnellan, S., Pennington, S. H., Ruggiero, A., Martinez-Rodriguez, C., Pouget, M., Thomas, J., …Paxton, W. A. (2023). A Quantitative Method for the Study of HIV-1 and Mycobacterium tuberculosis Coinfection. Journal of Infectious Diseases, 227(5), 708-713. https://doi.org/10.1093/infdis/jiac491

Journal Article Type Article
Acceptance Date Dec 15, 2022
Online Publication Date Dec 20, 2022
Publication Date Mar 1, 2023
Deposit Date Jan 11, 2023
Publicly Available Date Jan 11, 2023
Journal The Journal of Infectious Diseases
Print ISSN 0022-1899
Electronic ISSN 1537-6613
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 227
Issue 5
Pages 708-713
DOI https://doi.org/10.1093/infdis/jiac491
Keywords coinfection, drug screening, HIV-1, Mycobacterium tuberculosis, tuberculosis.

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