Measurement of the Intracellular Mycobacterium tuberculosis Drug Effect and Prediction of the Clinical Dose–Response Relationship Using Intracellular Pharmacodynamic Modeling (PDi)

Donnellan, Samantha; Mart�nez-Rodr�guez, Carmen; Aljayyoussi, Ghaith; Biagini, Giancarlo A.

Abstract

The human disease tuberculosis (TB) is the leading cause of death from a single infectious agent. A quarter of the world’s population is estimated to be latently infected. Drug development and screening is slow and costly. We have developed a physiologically relevant assay to screen drugs against TB when inside immune cells. This chapter will describe a newly developed preclinical drug screening assay for TB, using high-content imaging and pharmacokinetic/pharmacodynamic modeling.

Citation

Donnellan, S., Martínez-Rodríguez, C., Aljayyoussi, G., & Biagini, G. A. (2021). Measurement of the Intracellular Mycobacterium tuberculosis Drug Effect and Prediction of the Clinical Dose–Response Relationship Using Intracellular Pharmacodynamic Modeling (PDi). In C. Barreiro, & J.-L. Barredo (Eds.), Antimicrobial Therapies: Methods and Protocols (393-408). Humana Press. https://doi.org/10.1007/978-1-0716-1358-0

• Acceptance Date: Jan 18, 2021
• Online Publication Date: May 12, 2021
• Publication Date: Jul 12, 2021
• Deposit Date: Feb 28, 2022
• Publisher: Humana Press
• Pages: 393-408
• Series Title: Methods in Molecular Biology book series
• Series Number: 2296
• Series ISSN: 1064-3745
• Book Title: Antimicrobial Therapies: Methods and Protocols
• ISBN: 978-1-0716-1357-3
• DOI: https://doi.org/10.1007/978-1-0716-1358-0
• Keywords: Tuberculosis, Drug screening, Mycobacteria, Antibiotics, Pharmacokinetic and pharmacodynamic drug Modeling
• Public URL: http://researchrepository.napier.ac.uk/Output/2849464
• Publisher URL: https://link.springer.com/protocol/10.1007/978-1-0716-1358-0_23