Comprehensive two-dimensional liquid chromatography as a biomimetic screening platform for pharmacokinetic profiling of compound libraries in early drug development

Russo, Giacomo; Grumetto, Lucia; Baert, Mathijs; Lynen, Frederic

Abstract

A comprehensive two-dimensional liquid chromatography-based biomimetic platform (LCxLC) has been developed and validated for drug diffusion studies. Human serum albumin (HSA) and immobilized artificial membrane (IAM) were thereby used in the first (1D) and second (2D) separation dimension, respectively. While the former was meant to emulate the blood, the latter was instead intended to mimic the intestinal mucosa epithelium. Therefore, the experimental conditions, i.e. pH, temperature and buffer composition, were modulated to reflect faithfully in vivo conditions. 30 compounds, whose effective intestinal permeability (Peff) assayed in situ on humans by a validated technique was known from the literature, were used as model drugs.

A good and orthogonal separation was achieved for the whole dataset, although for a better distribution of the most polar compounds in the elution window a segmented gradient elution program had to be employed. Interestingly, the passively uptaken compounds having the most favourable Peff populated a specific area of the 2D plots, implying that the affinity for HSA and IAM has to lie in specific ranges in order for a compound to be satisfactorily absorbed from the intestinal lumen.

Although these results should be regarded as preliminary, this work paves an entirely new and unprecedented way to profile pharmaceutically relevant compounds for their in vivo absorption and distribution potential.

Citation

Russo, G., Grumetto, L., Baert, M., & Lynen, F. (2021). Comprehensive two-dimensional liquid chromatography as a biomimetic screening platform for pharmacokinetic profiling of compound libraries in early drug development. Analytica Chimica Acta, 1142, 157-168. https://doi.org/10.1016/j.aca.2020.11.003

• Journal Article Type: Article
• Acceptance Date: Nov 2, 2020
• Online Publication Date: Nov 11, 2020
• Publication Date: 2021-01
• Deposit Date: Nov 12, 2020
• Publicly Available Date: Nov 12, 2021
• Journal: Analytica Chimica Acta
• Print ISSN: 0003-2670
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 1142
• Pages: 157-168
• DOI: https://doi.org/10.1016/j.aca.2020.11.003
• Keywords: Immobilized artificial membrane, Human serum albumin, Effective intestinal permeability, Comprehensive two-dimensional liquid chromatography, ADMET profiling, Bioaffinity chromatography
• Public URL: http://researchrepository.napier.ac.uk/Output/2700946