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A preliminary evaluation of the differences in the glycosylation of alpha-1-acid glycoprotein between individual liver diseases.

Anderson, N E; Pollacchi, Aileen; Hayes, P; Therapondos, G; Newsome, P; Boyter, A; Smith, Kevin

Authors

N E Anderson

Aileen Pollacchi

P Hayes

G Therapondos

P Newsome

A Boyter

Kevin Smith



Abstract

During the acute phase response (APR) to tissue injury or infection, the liver is responsible for the level of mediators such as cytokines required at the site of inflammation and providing the essential components for wound healing and tissue repair. Additionally there are substantial alterations in the expression of plasma proteins of hepatic origin such as alpha-1-acid glycoprotein (AGP). The APR also results in alterations to the branching, sialylation and fucosylation of the oligosaccharide chains of AGP. This study investigated whether liver damage could be correlated with changes in AGP glycosylation in groups of patients with various liver diseases (alcoholic liver disease, hepatitis B, hepatitis C, cirrhosis). Hyperfucosylation occurred in all cases of liver disease, although the hepatitis B and C samples showed a more significant increase in comparison with the others. Additionally N-acetylgalactosamine (GalNAc) was detected in the majority of the hepatitis C samples, which was unexpected since this monosaccharide is not a usual component of the N-linked oligosaccharide chains. It was also determined by concanavalin (con) A chromatography that there is a shift towards the increased branching of the oligosaccharide chains in inflammatory liver diseases compared to normal serum.

Citation

Anderson, N. E., Pollacchi, A., Hayes, P., Therapondos, G., Newsome, P., Boyter, A., & Smith, K. (2002). A preliminary evaluation of the differences in the glycosylation of alpha-1-acid glycoprotein between individual liver diseases. Biomedical Chromatography, 16, 365-372. https://doi.org/10.1002/bmc.167

Journal Article Type Article
Publication Date Sep 1, 2002
Deposit Date Jul 2, 2008
Print ISSN 0269-3879
Electronic ISSN 1099-0801
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 16
Pages 365-372
DOI https://doi.org/10.1002/bmc.167
Keywords acute phase response; liver; cytokines; wound healing; tissue repair; alpha-1-acid glycoprotein; sialylation; fucosylation; oligosaccharide; alcoholic liver disease; hepatitis B; hepatitis C; cirrhosis;
Public URL http://researchrepository.napier.ac.uk/id/eprint/1673
Publisher URL http://dx.doi.org/10.1002/bmc.167