Luke Liddle
Venous occlusion during blood collection decreases plasma nitrite but not nitrate concentration in humans
Liddle, Luke; Burleigh, Mia C.; Monaghan, Chris; Muggeridge, David J.; Easton, Chris
Authors
Abstract
Background: To maintain vascular tone and blood flow when tissue oxygenation is reduced, nitrite anions are reduced to nitric oxide (NO). From a practical perspective, it is unclear how the application of a tourniquet during blood collection might influence measurement of NO metabolites. Accordingly, this study evaluated the effect of venous occlusion on plasma nitrite and nitrate during venous blood collection. Methods: Fifteen healthy participants completed two trials that were preceded by the ingestion of nitrate-rich beetroot juice (BRJ; total of ~8.4 mmol nitrate) or no supplementation (control). In both trials, blood was collected using a venepuncture needle while a tourniquet was applied to the upper arm and using an indwelling intravenous cannula, from the opposing arm. The venepuncture samples were collected at 35 s post occlusion. Changes in the oxygenation of forearm flexor muscles were assessed using near-infrared spectroscopy. Plasma nitrite and nitrate were analysed using gas-phase chemiluminescence. Results: In the control trial, plasma nitrite was significantly elevated when collected via the cannula (179 ± 67 nM) compared to venepuncture (112 ± 51 nM, P = 0.03). The ingestion of BRJ increased plasma nitrite and values remained higher when sampled from the cannula (473 ± 164 nM) compared to venepuncture (387 ± 136 nM, P < 0.001). Plasma nitrate did not differ between collection methods in either trial (all P > 0.05). The delta changes in total-, deoxy-, and oxy-haemoglobin were all significantly greater during venepuncture sample compared to the cannula sample at the point of blood collection (all P < 0.05). Conclusions: Venous occlusion during venepuncture blood collection lowers plasma nitrite concentration, potentially due to localised changes in haemoglobin concentration and/or a suppression of endogenous NO synthesis. Accordingly, the method of blood collection to enable measurements of NO metabolites should be carefully considered and consistently reported by researchers.
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 8, 2020 |
Online Publication Date | Jun 11, 2020 |
Publication Date | 2020-09 |
Deposit Date | Oct 22, 2020 |
Journal | Nitric Oxide |
Print ISSN | 1089-8603 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 102 |
Pages | 21-27 |
DOI | https://doi.org/10.1016/j.niox.2020.06.001 |
Keywords | Beetroot juice ,Cannula, Ischemia, Blood flow, Nitric oxide |
Public URL | http://researchrepository.napier.ac.uk/Output/2693998 |
You might also like
To clot or not to clot? That is a free radical question
(2018)
Journal Article
Untargeted Metabolomics Profiling of an 80.5 km Simulated Treadmill Ultramarathon
(2018)
Journal Article
Downloadable Citations
About Edinburgh Napier Research Repository
Administrator e-mail: repository@napier.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search