Dr David Muggeridge D.Muggeridge@napier.ac.uk
Lecturer
Dr David Muggeridge D.Muggeridge@napier.ac.uk
Lecturer
Daniel R. Crabtree
Ahmet Tuncay
Ian L. Megson
Gareth Davison
James N. Cobley
New readily accessible systemic redox biomarkers are needed to understand the biological roles reactive oxygen species (ROS) play in humans because overtly flawed, technically fraught, and unspecific assays severely hamper translational progress. The antibody-linked oxi-state assay (ALISA) makes it possible to develop valid ROS-sensitive target-specific protein thiol redox state biomarkers in a readily accessible microplate format. Here, we used a maximal exercise bout to disrupt redox homeostasis in a physiologically meaningful way to determine whether the catalytic core of the serine/threonine protein phosphatase PP2A is a candidate systemic redox biomarker in human erythrocytes. We reasoned that: constitutive oxidative stress (e.g., haemoglobin autoxidation) would sensitise erythrocytes to disrupted ion homeostasis as manifested by increased oxidation of the ion regulatory phosphatase PP2A. Unexpectedly, an acute bout of maximal exercise lasting ˜16 min decreased PP2A-specific reversible thiol oxidation (redox ratio, rest: 0.46; exercise: 0.33) without changing PP2A content (rest: 193 pg/ml; exercise: 191 pg/ml). The need for only 3–4 μl of sample to perform ALISA means PP2A-specific reversible thiol oxidation is a capillary—fingertip blood—compatible candidate redox biomarker. Consistent with biologically meaningful redox regulation, thiol reductant-inducible PP2A activity was significantly greater (+10%) at rest compared to exercise. We establish a route to developing new readily measurable protein thiol redox biomarkers for understanding the biological roles ROS play in humans.
Muggeridge, D. J., Crabtree, D. R., Tuncay, A., Megson, I. L., Davison, G., & Cobley, J. N. (2022). Exercise decreases PP2A-specific reversible thiol oxidation in human erythrocytes: Implications for redox biomarkers. Free Radical Biology and Medicine, 182, 73-78. https://doi.org/10.1016/j.freeradbiomed.2022.02.019
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 18, 2022 |
Online Publication Date | Feb 22, 2022 |
Publication Date | 2022-03 |
Deposit Date | Feb 23, 2022 |
Publicly Available Date | Feb 23, 2023 |
Journal | Free Radical Biology and Medicine |
Print ISSN | 0891-5849 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 182 |
Pages | 73-78 |
DOI | https://doi.org/10.1016/j.freeradbiomed.2022.02.019 |
Keywords | ALISA, Protein thiol, Biomarker, Redox signalling, Oxidative stress, Exercise, PP2A, Human |
Public URL | http://researchrepository.napier.ac.uk/Output/2847649 |
Exercise Decreases PP2A-specific Reversible Thiol Oxidation In Human Erythrocytes: Implications For Redox Biomarkers (accepted version)
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