Analysis of the elements of catabolite repression in Clostridium acetobutylicum.

Tangney, Martin; Deutscher, J; Galinier, A; Mitchell, Wilfred J

Abstract

The ptsH gene, encoding the phosphotransferase protein HPr, from Clostridium acetobutylicum ATCC 824 was identified from the genome sequence, cloned and shown to complement a ptsH mutant of Escherichia coli. The deduced protein sequence shares significant homology with HPr proteins from other low-GC gram-positive bacteria, although the highly conserved sequence surrounding the Ser-46 phosphorylation site is not well preserved in the clostridial protein. Nevertheless, the HPr was phosphorylated in an ATP-dependent manner in cell-free extracts of C. acetobutylicum. Furthermore, purified His-tagged HPr from Bacillus subtilis was also a substrate for the clostridial HPr kinase/phosphorylase. This phosphorylation reaction is a key step in the mechanism of carbon catabolite repression proposed to operate in B. subtilis and other low-GC gram-positive bacteria. Putative genes encoding the HPr kinase/phosphorylase and the other element of this model, namely the catabolite control protein CcpA, were identified from the C. acetobutylicum genome sequence, suggesting that a similar mechanism of carbon catabolite repression may operate in this industrially important organism.

Citation

Tangney, M., Deutscher, J., Galinier, A., & Mitchell, W. J. (2003). Analysis of the elements of catabolite repression in Clostridium acetobutylicum. Journal of Molecular Microbiology and Biotechnology, 6, 6-11. https://doi.org/10.1159/000073403

• Journal Article Type: Article
• Publication Date: Oct 1, 2003
• Deposit Date: Jul 4, 2008
• Print ISSN: 1464-1801
• Electronic ISSN: 1660-2412
• Publisher: Karger Publishers
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Pages: 6-11
• DOI: https://doi.org/10.1159/000073403
• Keywords: Phosphotransferase system; HPr; Protein kinase; CcpA
• Public URL: http://researchrepository.napier.ac.uk/id/eprint/1681
• Publisher URL: http://dx.doi.org/10.1159/000073403