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Characterisation of Staphylococcus aureus nasal
and skin carriage among patients undergoing
haemodialysis treatment.

Bogut, Agnieszka; Kozio?-Montewka, Maria; Baranowicz, Iwona; J�?wiak, Lucyna; Ksi?zek, Andrzej; Al-Doori, Zainab; Morrison, Donald; Kaczor, Danuta; Paluch-Ole?, Jolanta

Authors

Agnieszka Bogut

Maria Kozio?-Montewka

Iwona Baranowicz

Lucyna J�?wiak

Andrzej Ksi?zek

Zainab Al-Doori

Danuta Kaczor

Jolanta Paluch-Ole?



Abstract

The aim of the study was to investigate the rate of Staphylococcus aureus nasal and skin carriage in patients undergoing haemodialysis. The cultured staphylococcal isolates were subsequently characterized by molecular methods. The study group comprised 43 haemodialysed patients from whom nasal and skin swabs from the vascular access sites were collected. The identification of staphylococcal isolates and antibiotic susceptibility testing were performed on the basis of conventional diagnostic procedures. The staphylococci were further characterized using Pulsed-Field Gel Electrophoresis (PFGE). S. aureus was cultured from 12 (27.9%) patients. Only one (8.3%) patient was colonized with the microorganism both in the anterior nares and the vascular access site representing a single strain, as evidenced by PFGE analysis. Antibiotic susceptibility testing identified one (7.6%) methicillin-resistant S. aureus (MRSA) strain. PFGE typing identified several S. aureus genotypes with the lack of one specific strain responsible for colonization. However, it should be noted that among two (A and D) PFGE patterns genetically indistinguishable and closely related isolates (two isolates for each pattern) were identified. The obtained results revealed a relatively low rate of S. aureus carriage accompanied by low methicillin resistance rate and a significant genetic diversity of cultured isolates with the lack of one predominant strain responsible for colonization.

Citation

Bogut, A., Kozioł-Montewka, M., Baranowicz, I., Jóźwiak, L., Ksiązek, A., Al-Doori, Z., …Paluch-Oleś, J. (2007). Characterisation of Staphylococcus aureus nasal
and skin carriage among patients undergoing
haemodialysis treatment. The new microbiologica. 30. . 149-154. . ISSN 1121-7138.

Journal Article Type Article
Publication Date 2007-04
Deposit Date Jun 12, 2015
Print ISSN 1121-7138
Peer Reviewed Peer Reviewed
Volume 30
Pages 149-154
Keywords Staphylococcus aureus; nasal and skin carriage; haemodialysis;
Public URL http://researchrepository.napier.ac.uk/id/eprint/8690